2013
Characterization of Three Vasopressin Receptor 2 Variants: An Apparent Polymorphism (V266A) and Two Loss-of-Function Mutations (R181C and M311V)
Armstrong S, Seeber R, Ayoub M, Feldman B, Pfleger K. Characterization of Three Vasopressin Receptor 2 Variants: An Apparent Polymorphism (V266A) and Two Loss-of-Function Mutations (R181C and M311V). PLOS ONE 2013, 8: e65885. PMID: 23762448, PMCID: PMC3675069, DOI: 10.1371/journal.pone.0065885.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAquaporin 2Arginine VasopressinArrestinsbeta-ArrestinsChlorocebus aethiopsCOS CellsCyclic AMPDiabetes Insipidus, NephrogenicGene Expression RegulationGenetic Diseases, X-LinkedGTP-Binding Protein alpha Subunits, GsHEK293 CellsHumansInappropriate ADH SyndromeInositol PhosphatesMutationPolymorphism, GeneticReceptors, VasopressinSignal TransductionConceptsNephrogenic diabetes insipidusArginine vasopressinWild-type receptorAquaporin-2 water channelsActivation of Gs proteinsIncreased inositol phosphate productionPartial agonismVasopressin V2 receptorInositol phosphate accumulationGain-of-function mutationsIncreased cAMP levelsBRET assayInositol phosphate productionLoss-of-function mutationsNephrogenic syndromeV2R mutationsLoss-of-functionDiabetes insipidusMutant V2RV2 receptorsCAMP accumulationMilder phenotypePosterior pituitaryB-arrestinControls water homeostasis
2012
Activation of Soluble Adenylyl Cyclase Protects against Secretagogue Stimulated Zymogen Activation in Rat Pancreaic Acinar Cells
Kolodecik TR, Shugrue CA, Thrower EC, Levin LR, Buck J, Gorelick FS. Activation of Soluble Adenylyl Cyclase Protects against Secretagogue Stimulated Zymogen Activation in Rat Pancreaic Acinar Cells. PLOS ONE 2012, 7: e41320. PMID: 22844459, PMCID: PMC3402497, DOI: 10.1371/journal.pone.0041320.Peer-Reviewed Original ResearchConceptsProtein kinase AActivation of SACZymogen activationPancreatic acinar cellsSpecific subcellular domainsAcinar cellsActivation of zymogensCerulein-treated cellsSubcellular domainsDownstream targetsKinase ASAC activitySAC inhibitorAdenylyl cyclaseDistinct mechanismsAdenylyl cyclase inhibitorElevates levelsApical regionAmylase secretionCellsActivationAcinar cell vacuolizationCAMPCAMP accumulationCell vacuolization
2010
The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol
Yang Y, Shi Q, Liu Z, Li R, Pan P, Hou Y, Lu W, Bai G. The synergistic anti-asthmatic effects of glycyrrhizin and salbutamol. Acta Pharmacologica Sinica 2010, 31: 443-449. PMID: 20228825, PMCID: PMC4085675, DOI: 10.1038/aps.2009.207.Peer-Reviewed Original ResearchConceptsEffects of GLAnti-asthmatic effectsAnti-inflammatory effectsInterleukin-8Interleukin-8 ELISAMRNA levelsGuinea pig asthma modelInhibition of NF-kB activationIntracellular cAMP accumulationEfficacy of glycyrrhizinProduction of interleukin-8Anti-asthma therapyReal-time RT-PCRTreatment of asthmaCAMP in vitroBeta2-adrenergic receptorB2-ARNF-kB activationIncreased mRNA levelsCAMP accumulationCombination of GLAsthma modelSalbutamolRT-PCRWestern blotting
2000
Antibody-Induced Activation of β1 Integrin Receptors Stimulates cAMP-Dependent Migration of Breast Cells on Laminin-5
Plopper G, Huff J, Rust W, Schwartz M, Quaranta V. Antibody-Induced Activation of β1 Integrin Receptors Stimulates cAMP-Dependent Migration of Breast Cells on Laminin-5. Archives Of Biochemistry And Biophysics 2000, 4: 129-135. PMID: 11170844, DOI: 10.1006/mcbr.2001.0267.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine Diphosphate RiboseAntibodies, MonoclonalBreast NeoplasmsCell AdhesionCell Adhesion MoleculesCell MovementCells, CulturedCyclic AMPDNA PrimersFemaleHeterotrimeric GTP-Binding ProteinsHumansIntegrin alpha3beta1IntegrinsPertussis ToxinPrecipitin TestsReceptors, LamininSignal TransductionTumor Cells, CulturedVirulence Factors, Bordetella
1999
A G Protein-coupled Receptor from Zebrafish Is Activated by Human Parathyroid Hormone and Not by Human or Teleost Parathyroid Hormone-related Peptide IMPLICATIONS FOR THE EVOLUTIONARY CONSERVATION OF CALCIUM-REGULATING PEPTIDE HORMONES*
Rubin D, Hellman P, Zon L, Lobb C, Bergwitz C, Jüppner H. A G Protein-coupled Receptor from Zebrafish Is Activated by Human Parathyroid Hormone and Not by Human or Teleost Parathyroid Hormone-related Peptide IMPLICATIONS FOR THE EVOLUTIONARY CONSERVATION OF CALCIUM-REGULATING PEPTIDE HORMONES*. Journal Of Biological Chemistry 1999, 274: 23035-23042. PMID: 10438471, DOI: 10.1074/jbc.274.33.23035.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBiological EvolutionBlotting, SouthernCloning, MolecularDNA ProbesDNA, ComplementaryGTP-Binding ProteinsHumansIctaluridaeMolecular Sequence DataParathyroid HormoneParathyroid Hormone-Related ProteinProteinsRatsReceptor, Parathyroid Hormone, Type 2Receptors, Parathyroid HormoneRNA SplicingSequence Homology, Amino AcidZebrafishConceptsG protein-coupled receptorsAmino acid sequence identityProtein-coupled receptorsCAMP accumulationParathyroid hormoneAmino-terminal extracellular domainGrowth hormone-releasing hormoneCalcium-regulating peptide hormoneCDNA clonesHormone-releasing hormoneSequence identityParathyroid hormone 2 receptorHuman homologPTH/PTHrP receptorFamily of G protein-coupled receptorsHuman parathyroid hormoneAgonist-dependent activationSplice variantsCOS-7COS-7 cellsEncoding portionsExtracellular domainLigand specificityAmino acidsEvolutionary conservation
1995
Physiological and pharmacological inhibitors of luteinizing hormone-dependent steroidogenesis induce heat shock protein-70 in rat luteal cells
Khanna A, Aten R, Behrman H. Physiological and pharmacological inhibitors of luteinizing hormone-dependent steroidogenesis induce heat shock protein-70 in rat luteal cells. Endocrinology 1995, 136: 1775-1781. DOI: 10.1210/en.136.4.1775.Peer-Reviewed Original ResearchRat luteal cellsInduction of HSP-70Inducible HSP-70Luteal cellsHSP-70Progesterone synthesisHeat shock protein inductionPharmacological inhibitorsHeat shock transcriptional control elementTumor necrosis factor-alphaInhibition of progesterone synthesisHeat shock proteinsLuteal regressionPhorbol 12-myristate 13-acetateNecrosis factor-alphaProstaglandin F2 alphaAssociated with inhibitionFunctional luteal regressionCAMP accumulationFactor-alphaF2 alphaPGF2 alphaAlpha treatmentPGF2 alpha treatmentHeat shock protein 70
1994
Functional expression and characterization of human D2 and D3 dopamine receptors
Potenza M, Graminski G, Schmauss C, Lerner. Functional expression and characterization of human D2 and D3 dopamine receptors. Journal Of Neuroscience 1994, 14: 1463-1476. PMID: 7907363, PMCID: PMC6577580, DOI: 10.1523/jneurosci.14-03-01463.1994.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological AssayCyclic AMPDopamine AgentsDopamine AntagonistsHumansLigandsMelanocyte-Stimulating HormonesMelanophoresMelatoninPertussis ToxinPigments, BiologicalReceptors, DopamineReceptors, Dopamine D2Receptors, Dopamine D3TetrahydronaphthalenesVirulence Factors, BordetellaXenopus laevisConceptsD3 receptorsCAMP accumulationGi protein-coupled receptorHuman D2Selective dopaminergic agonistsDose-dependent mannerIC50 value determinationDopaminergic agonistsMelanocyte receptorsPertussis toxinD3 dopamineAgonistsReceptorsPigment granule aggregationAntagonistD2RXenopus laevis melanophoresSimilar concentrationsFunctional expressionPigment aggregationD3R.Initial feasibility studyFunctional characteristicsCultured Xenopus laevis melanophoresNew bioassay
1992
The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues.
Torchia J, Lytle C, Pon D, Forbush B, Sen A. The Na-K-Cl cotransporter of avian salt gland. Phosphorylation in response to cAMP-dependent and calcium-dependent secretogogues. Journal Of Biological Chemistry 1992, 267: 25444-25450. PMID: 1281159, DOI: 10.1016/s0021-9258(19)74061-7.Peer-Reviewed Original ResearchMeSH Keywords1-Methyl-3-isobutylxanthine8-Bromo Cyclic Adenosine MonophosphateAnimalsBlotting, WesternCalciumCarbacholCarrier ProteinsCell MembraneColforsinCyclic AMPDucksKineticsMembrane ProteinsPhosphatesPhosphorylationSalt GlandSodium-Potassium-Chloride SymportersTime FactorsVasoactive Intestinal PeptideConceptsNa-K-Cl cotransporterPhosphorylation of pp170Avian salt glandMonoclonal antibodiesResponse to carbacholEffect of forskolinNa-K-ClIntact cell preparationsConcentration-dependent increaseStimulation of secretionIncreased approximately 5-foldCAMP accumulationExposure of cellsCAMP-dependent protein kinasePp170Approximately 5-foldCell preparationsCAMP-dependentSalt gland cellsGland membranesWestern blottingForskolinSecretogoguesVIPAntibodies
1986
Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*
DELBEKE P, SCAMMELL J, MARTINEZ-CAMPOS A, DANNIES P. Dopamine Inhibits Prolactin Release when Cyclic Adenosine 3′,5′-Monophosphate Levels Are Elevated*. Endocrinology 1986, 118: 1271-1277. PMID: 3004906, DOI: 10.1210/endo-118-4-1271.Peer-Reviewed Original ResearchConceptsPRL releaseIntracellular cAMP concentrationFemale Fischer 344 ratsCAMP levelsAnterior pituitary cellsCAMP concentrationFischer 344 ratsPRL accumulationPercentage of lactotrophsDopamine inhibitsPituitary tumorsContinuous Percoll density gradientsNM TRHPituitary cellsCAMP accumulationMicroM dopaminePercoll density gradientsBasal levelsMonophosphate levelsMin of incubationLactotrophsAdenylate cyclasePrimary culturesDopamineForskolin
1983
Regulation of oocyte maturation in the mouse: Possible roles of intercellular communication, cAMP, and testosterone
Schultz R, Montgomery R, Ward-Bailey P, Eppig J. Regulation of oocyte maturation in the mouse: Possible roles of intercellular communication, cAMP, and testosterone. Developmental Biology 1983, 95: 294-304. PMID: 6186544, DOI: 10.1016/0012-1606(83)90030-1.Peer-Reviewed Original ResearchConceptsFollicle-stimulating hormoneCumulus cell-oocyte complexesCumulus cell-enclosed oocytesCholera toxinHeterologous gap junctionsCumulus cellsInhibitory effectCell cAMPCAMP contentMarked increaseCell typesPossible roleGap junctionsIntercellular communicationGroups of oocytesResumption of meiosisCAMP accumulationCAMP generationOocyte cAMPIntracellular cAMPOocyte maturationTestosteroneCAMP levelsCAMP degradationTransient inhibition
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