2007
Two Trypanosome-Specific Proteins Are Essential Factors for 5S rRNA Abundance and Ribosomal Assembly in Trypanosoma brucei▿
Hellman KM, Ciganda M, Brown SV, Li J, Ruyechan W, Williams N. Two Trypanosome-Specific Proteins Are Essential Factors for 5S rRNA Abundance and Ribosomal Assembly in Trypanosoma brucei▿. MSphere 2007, 6: 1766-1772. PMID: 17715362, PMCID: PMC2043393, DOI: 10.1128/ec.00119-07.Peer-Reviewed Original ResearchConceptsRNAi cellsRRNA levelsNovel nuclear RNATrypanosome-specific proteinsRNA interference studiesN-terminal regionRibosome biogenesisOverall protein synthesisRibosomal assemblyRibosome activityP37 proteinRRNA abundanceAncient familyNuclear RNARibosomal subunitTrypanosoma bruceiT. bruceiCell shapeGrowth arrestWeight complexesRRNAAcid differencesProtein p34Protein synthesisP34
2006
ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei
Brown SV, Hosking P, Li J, Williams N. ATP Synthase Is Responsible for Maintaining Mitochondrial Membrane Potential in Bloodstream Form Trypanosoma brucei. MSphere 2006, 5: 45-53. PMID: 16400167, PMCID: PMC1360250, DOI: 10.1128/ec.5.1.45-53.2006.Peer-Reviewed Original ResearchConceptsMitochondrial membrane potentialATP synthaseRNA interferenceBeta subunitProtein levelsAlpha subunitMembrane potentialBloodstream form T. bruceiSteady-state transcript levelsF1-ATPase alphaInduction of RNAiMitochondrion of TrypanosomaWild-type cellsBloodstream form TrypanosomaAlpha subunit transcriptsRNAi knockdownRNAi cellsT. bruceiTranscript levelsUninduced cellsBloodstream formsBeta subunit transcriptsSubunitsKrebs cycleFluorescence microscopy
2003
Stage-specific translational efficiency and protein stability regulate the developmental expression of p37, an RNA binding protein from Trypanosoma brucei
Li J, Ruyechan WT, Williams N. Stage-specific translational efficiency and protein stability regulate the developmental expression of p37, an RNA binding protein from Trypanosoma brucei. Biochemical And Biophysical Research Communications 2003, 306: 918-923. PMID: 12821129, DOI: 10.1016/s0006-291x(03)01084-2.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGene Expression RegulationPrecipitin TestsProtein BiosynthesisProtozoan ProteinsRecombinant ProteinsRNARNA-Binding ProteinsTime FactorsTrypanosoma brucei bruceiConceptsProcyclic stageP37 proteinVivo metabolic labelingRNA binding proteinPost-translational levelDifferential stabilityCysteine protease activityProcyclic cellsSignificant homologyBloodstream cellsDevelopmental regulationNovel RNAProtein stabilityTrypanosoma bruceiTranslational efficiencyMetabolic labelingPredominant proteinDevelopmental expressionBinding proteinP37P34ProteinProtease activityRNACells