2018
Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas
Kim Y, Terng EL, Riekhof WR, Cahoon EB, Cerutti H. Endoplasmic reticulum acyltransferase with prokaryotic substrate preference contributes to triacylglycerol assembly in Chlamydomonas. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: 1652-1657. PMID: 29382746, PMCID: PMC5816170, DOI: 10.1073/pnas.1715922115.Peer-Reviewed Original ResearchMeSH KeywordsAcyltransferasesAlgal ProteinsChlamydomonas reinhardtiiChloroplastsEndoplasmic ReticulumPhylogenySubstrate SpecificityTriglyceridesConceptsUnicellular green algaEndoplasmic reticulum membraneLysophosphatidic acid acyltransferaseChloroplastic pathwayPrecursor biosynthesisTAG precursorsNitrogen deprivationTriacylglycerol assemblyAssembly pathwayGreen algaTAG accumulationAcid acyltransferaseAcyl donor substratesReticulum membraneBiomaterial productionDonor substrateChlamydomonasTriacylglycerol metabolismPalmitoyl-CoAAcyltransferasePathwayMajor roleBiosynthesisAlgaRNA
2016
Identification of a Chlamydomonas plastidial 2‐lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content
Yamaoka Y, Achard D, Jang S, Legéret B, Kamisuki S, Ko D, Schulz‐Raffelt M, Kim Y, Song W, Nishida I, Li‐Beisson Y, Lee Y. Identification of a Chlamydomonas plastidial 2‐lysophosphatidic acid acyltransferase and its use to engineer microalgae with increased oil content. Plant Biotechnology Journal 2016, 14: 2158-2167. PMID: 27133096, PMCID: PMC5096022, DOI: 10.1111/pbi.12572.Peer-Reviewed Original ResearchConceptsAcid acyltransferaseNitrogen-deficient conditionsOil contentPlastid membranesBiosynthetic pathwayStorage lipidsBiosynthetic processesReinhardtii cellsMicroalgal oil productionMolecular toolsSn-2 positionEnzyme assaysAlgal lipidsPhosphatidic acidCommon precursorMicroalgaeAcyltransferaseCoAMembranePlastidsLPAATChlamydomonasReinhardtiiLipidsGenes