2018
Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In Vivo
Miller MB, Wilson RS, Lam TT, Nairn AC, Picciotto MR. Evaluation of the Phosphoproteome of Mouse Alpha 4/Beta 2-Containing Nicotinic Acetylcholine Receptors In Vitro and In Vivo. Proteomes 2018, 6: 42. PMID: 30326594, PMCID: PMC6313896, DOI: 10.3390/proteomes6040042.Peer-Reviewed Original ResearchProtein kinase APhosphorylation sitesPKA sitesHEK cellsNicotinic acetylcholine receptorsCalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IIProtein kinase IIAcetylcholine receptorsKinase AKinase IIMouse brainSubunit phosphorylationNovel siteSubunitsAcute nicotine administrationΒ2 subunitMammalian brainCaMKIIPhosphorylationReceptor functionΑ4 subunitHuman brain tissueBeta 2Nicotine administration
2005
GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP‐dependent mechanism
Hawes JJ, Brunzell DH, Wynick D, Zachariou V, Picciotto MR. GalR1, but not GalR2 or GalR3, levels are regulated by galanin signaling in the locus coeruleus through a cyclic AMP‐dependent mechanism. Journal Of Neurochemistry 2005, 93: 1168-1176. PMID: 15934937, PMCID: PMC1352153, DOI: 10.1111/j.1471-4159.2005.03105.x.Peer-Reviewed Original ResearchConceptsCAMP-dependent mannerKnockout micePhysiological functionsCREB phosphorylationProtein levelsGALR1 expressionCell linesGalanin knockout miceMRNA levelsCAMP levelsMouse brainCyclic AMP-dependent mechanismGalR3Important rolePhosphorylationGalR1GalR2ProteinNegative feedbackMiceExpressionLevelsNucleusCATH
2004
Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain
Hawes JJ, Picciotto MR. Characterization of GalR1, GalR2, and GalR3 immunoreactivity in catecholaminergic nuclei of the mouse brain. The Journal Of Comparative Neurology 2004, 479: 410-423. PMID: 15514977, DOI: 10.1002/cne.20329.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainCatecholaminesGalaninImmunohistochemistryLocus CoeruleusMiceNeural PathwaysNeuronsNucleus AccumbensOpioid-Related DisordersReceptor, Galanin, Type 1Receptor, Galanin, Type 2Receptor, Galanin, Type 3Receptors, GalaninRewardSubstantia NigraTyrosine 3-MonooxygenaseVentral Tegmental AreaConceptsVentral tegmental areaSubstantia nigraLocus coeruleusNucleus accumbensGalanin receptorsBrain areasMouse brainDistribution of immunoreactivityNoradrenergic transmissionGalanin bindingOpiate withdrawalTegmental areaCatecholaminergic nucleiTyrosine hydroxylaseDopamine neurotransmissionGalR1GalR2GalR3BrainProtein levelsDrug addictionGalaninImmunoreactivityReceptorsCoeruleus
2002
Characterization of [125I]epibatidine binding and nicotinic agonist‐mediated 86Rb+ efflux in interpeduncular nucleus and inferior colliculus of β2 null mutant mice
Marks MJ, Whiteaker P, Grady SR, Picciotto MR, McIntosh JM, Collins AC. Characterization of [125I]epibatidine binding and nicotinic agonist‐mediated 86Rb+ efflux in interpeduncular nucleus and inferior colliculus of β2 null mutant mice. Journal Of Neurochemistry 2002, 81: 1102-1115. PMID: 12065623, DOI: 10.1046/j.1471-4159.2002.00910.x.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAlkaloidsAnimalsAzocinesBinding, CompetitiveBridged Bicyclo Compounds, HeterocyclicDose-Response Relationship, DrugInferior ColliculiIodine RadioisotopesMesencephalonMiceMice, Mutant StrainsNicotinic AgonistsNicotinic AntagonistsPyridinesQuinolizinesReceptors, NicotinicRubidium RadioisotopesTritiumConceptsInterpeduncular nucleusInferior colliculusBrain regionsAccessory olfactory nucleusNull mutant miceOlfactory nucleusNicotinic antagonistsD-tubocurarineMedial habenulaSelective antagonistNicotinic agonistsSuperior colliculusMouse brainAgonistsColliculusMutant micePotent agonistSimilar potencyAntagonistNicotinic activityEfflux
2000
Brain Localization and Behavioral Impact of the G-Protein-Gated K+ Channel Subunit GIRK4
Wickman K, Karschin C, Karschin A, Picciotto M, Clapham D. Brain Localization and Behavioral Impact of the G-Protein-Gated K+ Channel Subunit GIRK4. Journal Of Neuroscience 2000, 20: 5608-5615. PMID: 10908597, PMCID: PMC6772558, DOI: 10.1523/jneurosci.20-15-05608.2000.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAvoidance LearningBehavior, AnimalBrain ChemistryChick EmbryoFemaleG Protein-Coupled Inwardly-Rectifying Potassium ChannelsGene ExpressionIn Situ HybridizationIon Channel GatingLocomotionMaleMaze LearningMemoryMiceMice, Inbred C57BLMice, KnockoutPotassium ChannelsPotassium Channels, Inwardly RectifyingRNA, MessengerConceptsGIRK4 mRNAG-protein-gated potassium (GIRK) channelsCortical pyramidal neuronsVentromedial hypothalamic nucleusParaventricular thalamic nucleusMorris water mazeG-Protein-GatedPassive avoidance paradigmMammalian nervous systemWild-type controlsEndopiriform nucleusPyramidal neuronsGlobus pallidusSynaptic inhibitionBrainstem nucleiHypothalamic nucleiPain perceptionThalamic nucleiInsular cortexNervous systemNeuronal populationsWater mazeLocomotor activityMouse brainGIRK subunits
1999
Two pharmacologically distinct components of nicotinic receptor-mediated rubidium efflux in mouse brain require the beta2 subunit.
Marks MJ, Whiteaker P, Calcaterra J, Stitzel JA, Bullock AE, Grady SR, Picciotto MR, Changeux JP, Collins AC. Two pharmacologically distinct components of nicotinic receptor-mediated rubidium efflux in mouse brain require the beta2 subunit. Journal Of Pharmacology And Experimental Therapeutics 1999, 289: 1090-103. PMID: 10215692.Peer-Reviewed Original ResearchConceptsBeta2 subunitBeta2 null mutant miceConcentration-effect curvesMouse brain synaptosomesAlpha4beta2 receptorsBrain synaptosomesNicotinic agonistsMouse brainRubidium effluxMutant miceLine radioactivity detectionDHbetaEAgonistsEffluxBrainStimulationRadioactivity detectionPotencyHexamethoniumErythroidineResponseAcetylcholineMethyllycaconitineAntagonistBungarotoxin