1994
Subcellular localization of CFTR to endosomes in a ductal epithelium
Webster P, Vanacore L, Nairn A, Marino C. Subcellular localization of CFTR to endosomes in a ductal epithelium. American Journal Of Physiology 1994, 267: c340-c348. PMID: 7521124, DOI: 10.1152/ajpcell.1994.267.2.c340.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MembraneCystic FibrosisCystic Fibrosis Transmembrane Conductance RegulatorEndocytosisEpitheliumFluorescent Antibody TechniqueImmunohistochemistryMaleMembrane ProteinsMicroscopy, FluorescenceOrganellesRatsRats, Sprague-DawleyReceptors, Cell SurfaceSubcellular FractionsSubmandibular GlandTissue DistributionConceptsCystic fibrosis transmembrane conductance regulatorPlasma membraneFibrosis transmembrane conductance regulatorApical plasma membraneAnti-CFTR antibodiesNormal epithelial cell populationsTransmembrane conductance regulatorCytochemical evidenceReceptor-mediated endocytosisCFTR moleculesEpithelial cell populationsCellular processesSubcellular compartmentsSubcellular localizationEarly endosomesMembrane recyclingConductance regulatorSubcellular distributionSubapical vesiclesApical poleEndosomesCFTR functionImmunoelectron microscopyCell populationsCFTR immunoreactivity
1992
Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line.
Cohn J, Nairn A, Marino C, Melhus O, Kole J. Characterization of the cystic fibrosis transmembrane conductance regulator in a colonocyte cell line. Proceedings Of The National Academy Of Sciences Of The United States Of America 1992, 89: 2340-2344. PMID: 1372442, PMCID: PMC48653, DOI: 10.1073/pnas.89.6.2340.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorFibrosis transmembrane conductance regulatorTransmembrane conductance regulatorConductance regulatorTwo-dimensional phosphopeptide mappingT84 cellsProtein kinase ACell linesProtein kinase CSDS/PAGEPhosphopeptide mappingPhosphorylation sitesProminent substrateCFTR peptidesEquivalent proteinsKinase ASame proteinKinase CTerminal sequenceCell lysatesN-glycanaseProteinAnti-peptide antibodiesImmunoblot signalsCFTR immunoreactivity