Genetics; Ion Channels; Neuropeptides; Neurophysiology; Neurotoxins; Physiology
Our laboratory applies cellular, molecular, genetic, and systems biology approaches to the question of how neuronal physiological properties determine the information processing characteristics of neural networks. We take an interdisciplinary approach to these questions. We manipulate the physiological properties of neurons in directed ways by genetically targeted cell-specific expression of engineered proteins in transgenic animals. These engineered proteins include ion channel subunits, intracellular ionic buffers, signaling enzymes, membrane-tethered neuropeptides, and membrane-tethered peptide neurotoxins that target specific ion channel subtypes. Subsequently, we measure the effects of these manipulations on the whole-animal behavior of intact flies as well as on various physiological parameters of the manipulated neurons using cell biological, neurophysiological, functional imaging, and genomics/systems biology techniques. As model systems for addressing these issues, we study the neural circuits that control circadian rhythms of locomotor activity, sexual courtship behavior, sleep, energy metabolism, and decision making in Drosophila melanogaster flies and Caenorhabditis elegans worms.
We also have a new project in the laboratory aimed at identifying novel analgesics from the venom of Australian funnel-web spiders.
Specialized Terms: Neurophysiology; Molecular genetics; Systems Physiology; Animal Behavior
- Cao, G., Platisa, J., Pieribone, V.A., Raccuglia, D., Kunst, M., Nitabach, M.N. (2013). Genetically targeted optical electrophysiology in intact neural circuits. Cell 154: 904-13.
- Gui, J., Liu, B., Cao, G., Lipchik, A.M., Perez, M., Dekan, Z., Mobli, M., Daly, N.L., Alewood, P.F., Parker, L.L., King, G.F., Zhou, Y., Jordt, S.-E., Nitabach, M.N. (2014). A tarantula-venom peptide antagonises the TRPA1 nociceptor ion channel by binding to the S1-S4 gating domain. Curr. Biol. 24: 473-83. [PMCID: PMC3949122] [covered by The New York Times: http://www.nytimes.com/2014/02/18/science/the-peruvian-green-velvet-tarantulas-gift.html]
- Kunst, M., Hughes, M.E., Raccuglia, D., Felix, M., Li, M., Barnett, G., Duah, J., Nitabach, M.N. (in press). CGRP neurons mediate sleep-specific circadian output in Drosophila. Curr. Biol..
- Choi, C., Cao, C., Tanenhaus, A.K., McCarthy, E.v., Jung, M., Schleyer, W., Shang, Y., Rosbash, M., Yin, J.C.P., Nitabach, M.N. (2012). Autoreceptor modulation of peptide/neurotransmitter co-release from PDF neurons determines allocation of circadian activity in Drosophila. Cell Rep. 2: 332-44.
- Hughes, M.E., Grant, G.R., Paquin, C., Qian, J., Nitabach, M.N. (2012). Deep sequencing the circadian and diurnal transcriptome of Drosophila brain. Genome Res. 22: 1266-81.
- Taghert, P.H. and Nitabach, M.N. (2012). Peptide neuromodulation in invertebrate model systems. Neuron 76: 82-97.