Jing Yan, PhD
Assistant Professor, Molecular, Cellular and Developmental BiologyCards
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Featured Publications
Bacteria surfing the elastic wave
Nijjer J, Cohen T, Yan J. Bacteria surfing the elastic wave. Nature Physics 2022, 19: 6-7. DOI: 10.1038/s41567-022-01862-y.Peer-Reviewed Original ResearchSocial evolution of shared biofilm matrix components
Tai J, Mukherjee S, Nero T, Olson R, Tithof J, Nadell C, Yan J. Social evolution of shared biofilm matrix components. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2123469119. PMID: 35771939, PMCID: PMC9271185, DOI: 10.1073/pnas.2123469119.Peer-Reviewed Original ResearchConceptsBiofilm matrix componentsCooperative public goodsMatrix componentsBiofilm formationBiofilm matrix proteinsMatrix productionSocial evolution theoryBiofilm spatial structureCell surface adhesionEvolutionary timescalesCell clustersRelatedness coefficientsBacterial communitiesBiofilm habitatAdhesion proteinsEvolutionary advantageEvolutionary stabilityMatrix proteinsAssociated analysis toolsDiffusible compoundsHost environmentSocial evolutionUbiquitous modeOutstanding questionsProteinMechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix
Zhang Q, Nguyen D, Tai J, Xu X, Nijjer J, Huang X, Li Y, Yan J. Mechanical Resilience of Biofilms toward Environmental Perturbations Mediated by Extracellular Matrix. Advanced Functional Materials 2022, 32 DOI: 10.1002/adfm.202110699.Peer-Reviewed Original ResearchMechanical resilienceNonlinear viscoelastic behaviorMaterials science pointLarge shear forcesBiofilm removal strategiesMechanical behaviorViscoelastic materialsViscoelastic behaviorBiofilm mechanicsSoft materialsStructure-property relationshipsRheological regimesRheological measurementsShear forceFunctional soft materialsLarge mechanical perturbationsPhysical insightPolymeric networkRemoval strategiesDynamics simulationsMolecular dynamics simulationsMaterialsDynamic environmentMatrixMechanical perturbationsMechanical forces drive a reorientation cascade leading to biofilm self-patterning
Nijjer J, Li C, Zhang Q, Lu H, Zhang S, Yan J. Mechanical forces drive a reorientation cascade leading to biofilm self-patterning. Nature Communications 2021, 12: 6632. PMID: 34789754, PMCID: PMC8599862, DOI: 10.1038/s41467-021-26869-6.Peer-Reviewed Original ResearchConceptsVibrio cholerae biofilmsSurface-attached aggregatesBacterial communitiesCell-surface interactionsDevelopmental processesBiofilm developmentBacterial cellsCell reorientationExtracellular matrixNonadherent mutantsDifferential growthBacterial biofilmsMechanical forcesBiofilmsBacterial growthDifferential orderingCellsGrowthMutantsLarge collectionMorphogenesis and cell ordering in confined bacterial biofilms
Zhang Q, Li J, Nijjer J, Lu H, Kothari M, Alert R, Cohen T, Yan J. Morphogenesis and cell ordering in confined bacterial biofilms. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2107107118. PMID: 34330824, PMCID: PMC8346881, DOI: 10.1073/pnas.2107107118.Peer-Reviewed Original ResearchConceptsInherent developmental programsBiofilm-dwelling cellsBiofilm-forming speciesSingle-cell imagingDevelopmental programSpecific matrix componentsCell orderingBiofilm developmentSpherical biofilmsBacterial cellsCell communityExtracellular matrixBiofilm morphologyBacterial biofilmsBiophysical mechanismsMatrix componentsHost tissuesBiofilmsBiofilm growthStiff gelsCellsMorphogenesisMutagenesisSpeciesVibrio
2025
Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera.
Verma S, Cetinbas M, Kelly M, Senger S, Faherty C, Janardhanan J, Wagh C, Bhuiyan T, Chowdhury F, Khan A, Akter A, Charles R, Harris J, Calderwood S, Wrammert J, Waldor M, Asp M, Tai J, Yan J, Xu P, Kováč P, Sadreyev R, Qadri F, Ryan E. Human antibody targeting Vibrio cholerae O1 O-specific polysaccharide induces an amotile hypovirulent bacterial phenotype: mechanism of protection against cholera. MBio 2025, e0223525. PMID: 40937849, DOI: 10.1128/mbio.02235-25.Peer-Reviewed Original ResearchAnti-OSP antibodiesO-specific polysaccharideVibrio cholerae,Bacterial motilityLevels of cyclic di-GMPInhibition of bacterial motilityEnteroid modelCyclic di-GMPCholera toxin detectionIntestinal surfaceCulture-based assaysInhibition of motilityVirulence programInfect humansRegulon expressionDi-GMPVirulence genesVirulence mechanismsBacterial phenotypesPathogen physiologyPresence of mucinExtracellular matrix componentsBiofilm formationBacterial metabolismIntestinal mucusDissecting the physics of bacterial biofilms with agent-based simulations
Nam K, Li C, Cockx B, Nguyen D, Li Y, Kreft J, Yan J. Dissecting the physics of bacterial biofilms with agent-based simulations. Current Opinion In Solid State And Materials Science 2025, 37: 101228. PMID: 40538632, PMCID: PMC12176386, DOI: 10.1016/j.cossms.2025.101228.Peer-Reviewed Original ResearchVibrio cholerae biofilmsBiofilm developmentExtracellular matrixDevelopment of bacterial coloniesSingle-cell resolutionBacterial communitiesCellular organizationBacterial coloniesBacterial biofilmsBiofilmBiological entitiesDevelopment of modeling approachesOrientational orderPhysical mechanismsExternal perturbationsComplex networksVibrioMechanistic originComprehensive genomic and evolutionary analysis of biofilm matrix clusters and proteins in the Vibrio genus
Yang Y, Yan J, Olson R, Jiang X. Comprehensive genomic and evolutionary analysis of biofilm matrix clusters and proteins in the Vibrio genus. MSystems 2025, 10: e00060-25. PMID: 40207939, PMCID: PMC12090793, DOI: 10.1128/msystems.00060-25.Peer-Reviewed Original ResearchConceptsGene clusterVibrio speciesEvolutionary analysisBiofilm dispersalBiofilm matrix proteinsVibrio choleraeGenetic modificationTail proteinsVibrio genusCholera pathogenIdentified genesGene groupsVibrio cholerae.Host functionsEvolutionary patternsBiofilm diversityBiofilm formationBiofilm proteinsBiofilm matrixVibrioBiofilm developmentEngineered biofilmsGenusStructural domainsAcute diarrheal diseaseNon-disruptive matrix turnover is a conserved feature of biofilm aggregate growth in paradigm pathogenic species
Reichhardt C, Matwichuk M, Lewerke L, Jacobs H, Yan J, Parsek M. Non-disruptive matrix turnover is a conserved feature of biofilm aggregate growth in paradigm pathogenic species. MBio 2025, 16: e03935-24. PMID: 39982068, PMCID: PMC11898600, DOI: 10.1128/mbio.03935-24.Peer-Reviewed Original ResearchConceptsSynthesize EPSBiofilm aggregatesBiofilm matrixBiofilm matrix exopolysaccharidesBiofilm-forming speciesBiofilm matrix compositionStudy of biofilmsMatrix exopolysaccharidesVibrio cholerae.Cellular biomassPathogenic speciesModel organismsBacterial speciesExtracellular DNAExopolysaccharideBiofilm stabilityMulticellular aggregatesCell-free matricesBiofilmCell-free formSpeciesMatrix turnoverAggregate peripheryBacteriaMatrix componentsMorphogenesis of confined biofilms: how mechanical interactions determine cellular patterning and global geometry
Nam K, Yan J. Morphogenesis of confined biofilms: how mechanical interactions determine cellular patterning and global geometry. Soft Matter 2025, 21: 1436-1450. PMID: 39901805, PMCID: PMC11791476, DOI: 10.1039/d4sm01180e.Peer-Reviewed Original ResearchConceptsVibrio cholerae biofilmsBiofilm developmentTransmission of mechanical stressExtracellular matrixSingle-cell levelBacterial communitiesDevelopmental biologyBacterial coloniesBiofilmSingle-cellReview recent studiesMechanical interplayFluorescence microscopyMorphogenesisActive nematicsGlobal geometryCellular patternOrientational orderContinuum levelVibrioConfining mediumRecent studiesMechanical interaction
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- Reconstructed 3D image of a Vibrio cholerae biofilm cluster. Color corresponds to height.
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Yale University
C144 Yale Science Building, 260 Whitney Ave.
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