2024
A newly identified gene Ahed plays essential roles in murine haematopoiesis
Nakai R, Yokota T, Tokunaga M, Takaishi M, Yokomizo T, Sudo T, Shi H, Yasumizu Y, Okuzaki D, Kokubu C, Tanaka S, Takaoka K, Yamanishi A, Yoshida J, Watanabe H, Kondoh G, Horie K, Hosen N, Sano S, Takeda J. A newly identified gene Ahed plays essential roles in murine haematopoiesis. Nature Communications 2024, 15: 5090. PMID: 38918373, PMCID: PMC11199565, DOI: 10.1038/s41467-024-49252-7.Peer-Reviewed Original ResearchConceptsMutant embryonic stem cellsConditional knockoutUncharacterised genesHaematopoietic cellsNuclear proteinsFunctional genesHaematopoiesis in vivoAssociated with malignancyBiological functionsGenesSomatic mutationsEmbryonic stem cellsHaematopoietic developmentTransplantation experimentsHaematological malignanciesDeletionAdult miceCancer patientsCoordinated actionEmbryonic dayStem cellsHaematopoiesisMurine haematopoiesisCellsMalignancy
2023
IL‐27 produced during acute malaria infection regulates Plasmodium‐specific memory CD4+ T cells
Macalinao M, Inoue S, Tsogtsaikhan S, Matsumoto H, Bayarsaikhan G, Jian J, Kimura K, Yasumizu Y, Inoue T, Yoshida H, Hafalla J, Kimura D, Yui K. IL‐27 produced during acute malaria infection regulates Plasmodium‐specific memory CD4+ T cells. EMBO Molecular Medicine 2023, 15: emmm202317713. PMID: 37855243, PMCID: PMC10701605, DOI: 10.15252/emmm.202317713.Peer-Reviewed Original ResearchConceptsCD4<sup>+</sup> T cellsT cellsIL-27Malaria infectionCD4<sup>+</sup> T cell responsesCD4<sup>+</sup> T cell subsetsMemory CD4+ T cellsImmune responseCD4+ T cellsNeutralization of IL-27T cell responsesT cell subsetsPathogenic immune responsesHumoral immune responseSingle-cell RNA-seq analysisPlasmodium chabaudiDevelopment of vaccinesAcute infectionCytokine productionEffector responsesChronic phaseActive infectionProliferative capacityAcute phaseInfection
2022
CCR8-targeted specific depletion of clonally expanded Treg cells in tumor tissues evokes potent tumor immunity with long-lasting memory
Kidani Y, Nogami W, Yasumizu Y, Kawashima A, Tanaka A, Sonoda Y, Tona Y, Nashiki K, Matsumoto R, Hagiwara M, Osaki M, Dohi K, Kanazawa T, Ueyama A, Yoshikawa M, Yoshida T, Matsumoto M, Hojo K, Shinonome S, Yoshida H, Hirata M, Haruna M, Nakamura Y, Motooka D, Okuzaki D, Sugiyama Y, Kinoshita M, Okuno T, Kato T, Hatano K, Uemura M, Imamura R, Yokoi K, Tanemura A, Shintani Y, Kimura T, Nonomura N, Wada H, Mori M, Doki Y, Ohkura N, Sakaguchi S. CCR8-targeted specific depletion of clonally expanded Treg cells in tumor tissues evokes potent tumor immunity with long-lasting memory. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2114282119. PMID: 35140181, PMCID: PMC8851483, DOI: 10.1073/pnas.2114282119.Peer-Reviewed Original ResearchConceptsTumor TregsTumor immunityMAb treatmentTumor tissuesT cellsLong-lasting memoryCD4<sup>+</sup> regulatory T cellsPD-1 immune checkpoint blockadeCures of established tumorsInduce severe autoimmune diseaseSuppress antitumor immune responsesImmune responseT cell receptor clonotypesImmune checkpoint blockadeSystemic Treg depletionAntitumor immune responseConventional T cellsRegulatory T cellsTumor-associated antigensChemokine receptor CCR8Several autoimmune diseasesSecondary immune responseTreg depletionCheckpoint blockadePD-1
2021
Localization of KRAS downstream target ARL4C to invasive pseudopods accelerates pancreatic cancer cell invasion
Harada A, Matsumoto S, Yasumizu Y, Shojima K, Akama T, Eguchi H, Kikuchi A. Localization of KRAS downstream target ARL4C to invasive pseudopods accelerates pancreatic cancer cell invasion. ELife 2021, 10: e66721. PMID: 34590580, PMCID: PMC8598236, DOI: 10.7554/elife.66721.Peer-Reviewed Original ResearchConceptsDownstream effectorsEGF-Ras pathwayCancer cell invasionPancreatic cancer patientsPancreatic cancer cellsIQGAP1ARL4CCell invasionPancreatic cancerSite of invasionCancer cellsCancer patientsExtracellular matrixPseudopodsAntisense oligonucleotidesInvasive pancreatic cancerMetastasis of pancreatic cancerActive siteInduced degradationEffectorMMP14InvasionMortality rateLocalizationOligonucleotidesAnti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats
Ishikura T, Kinoshita M, Shimizu M, Yasumizu Y, Motooka D, Okuzaki D, Yamashita K, Murata H, Beppu S, Koda T, Tada S, Shiraishi N, Sugiyama Y, Miyamoto K, Kusunoki S, Sugimoto T, Kumanogoh A, Okuno T, Mochizuki H. Anti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats. Journal Of Neuroinflammation 2021, 18: 181. PMID: 34419102, PMCID: PMC8380350, DOI: 10.1186/s12974-021-02232-w.Peer-Reviewed Original ResearchConceptsNeuromyelitis optica spectrum disorderIgG-treated ratsMechanical allodyniaNeuropathic painSpinal cordATP receptorsATP releaseSymptoms of neuromyelitis optica spectrum disorderDevelopment of mechanical allodyniaDevelopment of neuropathic painPharmacological inhibitionDevelopment of painful symptomsAnti-AQP4 autoantibodiesPeripheral neuropathic painAnti-AQP4 antibodyRelease of extracellular ATPExtracellular ATP releaseRat spinal cordDamage-associated molecular patternsAnti-AQP4Pain mechanismsMechanical painInvolvement of ATPPain symptomsRemission phase
2020
Proenkephalin+ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function
Shime H, Odanaka M, Tsuiji M, Matoba T, Imai M, Yasumizu Y, Uraki R, Minohara K, Watanabe M, Bonito A, Fukuyama H, Ohkura N, Sakaguchi S, Morita A, Yamazaki S. Proenkephalin+ regulatory T cells expanded by ultraviolet B exposure maintain skin homeostasis with a healing function. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 20696-20705. PMID: 32769209, PMCID: PMC7456133, DOI: 10.1073/pnas.2000372117.Peer-Reviewed Original ResearchConceptsTreg cellsUltraviolet B exposureUnique TCR repertoireFoxp3 transcription factorRegulatory T cellsSkin homeostasisSkin explant assayImmunological self-toleranceFunctional regulatory TUltraviolet BWound healingSuppress various immune responsesEndogenous opioid precursorsSkin TregsExpressed CD25Regulatory TTCR repertoireProenkephalin expressionT cellsSelf-toleranceWound closure assayImmune responseTregsInflammatory responseMet-Enk