Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution
Matsuo Y, Komiya S, Yasumizu Y, Yasuoka Y, Mizushima K, Takagi T, Kryukov K, Fukuda A, Morimoto Y, Naito Y, Okada H, Bono H, Nakagawa S, Hirota K. Full-length 16S rRNA gene amplicon analysis of human gut microbiota using MinION™ nanopore sequencing confers species-level resolution. BMC Microbiology 2021, 21: 35. PMID: 33499799, PMCID: PMC7836573, DOI: 10.1186/s12866-021-02094-5.Peer-Reviewed Original ResearchMeSH KeywordsBacteriaDNA, BacterialFecesGastrointestinal MicrobiomeHigh-Throughput Nucleotide SequencingHumansNanopore SequencingRNA, Ribosomal, 16SConceptsRRNA gene amplicon sequencingShort-read sequencingHuman gut microbiotaGene amplicon sequencingAmplicon sequencingRibosomal RNAGut microbiotaTaxonomic classificationBacterial communitiesBacterial compositionFull-length 16S rRNA geneShort-read sequencing methodsConventional short-read sequencing methodsDominant bacterial generaMock bacterial communityLong-read sequencingTaxonomic classification of bacteriaComplex bacterial communitiesRRNA gene analysisClassification of bacteriaComplex bacterial compositionHuman fecal samplesRRNA geneNanopore sequencingMicrobiome studies