2000
Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro
Pan Z, Zhang D, Zhang X, Lipton S. Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro. European Journal Of Neuroscience 2000, 12: 3137-3145. PMID: 10998097, DOI: 10.1046/j.1460-9568.2000.00198.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDose-Response Relationship, DrugElectrophysiologyGABA AntagonistsGamma-Aminobutyric AcidGene ExpressionIn Vitro TechniquesMutagenesisNeural InhibitionOocytesPicrotoxinProtein Structure, TertiaryRatsReceptors, GABAReceptors, GABA-AReceptors, GABA-BReceptors, GlycineRetinaXenopus laevisConceptsGlycine alpha1Rho1 subunitAlpha2 subunitHeteromeric receptorsGABA dose-response curveBlockade of GABAVoltage-clamp recordingsGamma-aminobutyric acidXenopus laevis oocyte expression systemTwo-electrode voltage-clamp recordingsDose-response curveResponse propertiesPicrotoxinin sensitivityOocyte expression systemGamma2 subunitGABAReceptor subunitsPharmacological propertiesReceptorsUnique gatingFunctional evidenceGABAAAlpha1Gamma2Homomeric
1995
Cloning of a gamma-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block.
Zhang D, Pan Z, Zhang X, Brideau A, Lipton S. Cloning of a gamma-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11756-11760. PMID: 8524843, PMCID: PMC40481, DOI: 10.1073/pnas.92.25.11756.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceChloride ChannelsCloning, MolecularDose-Response Relationship, DrugDrug ResistanceElectric ConductivityGABA AntagonistsIon ChannelsMethionineMolecular Sequence DataMutagenesis, Site-DirectedPicrotoxinProtein ConformationRatsReceptors, GABARetinaSequence Homology, Amino AcidSesterterpenesStructure-Activity RelationshipConceptsGABAC responsesRat retinaRho 2 subunitsGamma-aminobutyric acidPTX resistanceHeteromeric receptorsReceptor subunitsFunctional homomeric receptorsRho 1 receptorsGABA receptor subunitsInhibitory neurotransmissionMechanism of blockGABA receptorsMammalian retinaConvulsant picrotoxininHomomeric receptorsRat receptorRetinaIonotropic receptorsReceptorsChannel blockPicrotoxininSecond membrane-spanning regionNative receptorPredominant determinant
1989
GABA-activated single channel currents in outside-out membrane patches from rat retinal ganglion cells
Lipton S. GABA-activated single channel currents in outside-out membrane patches from rat retinal ganglion cells. Visual Neuroscience 1989, 3: 275-279. PMID: 2487107, DOI: 10.1017/s0952523800010026.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGamma-aminobutyric acidGanglion cellsWhole-cell currentsMammalian retinal ganglion cellsRat retinal ganglion cellsLarge whole-cell currentsMammalian central nervous systemWhole-cell recordingsCentral nervous systemPatch-clamp methodInhibitory transmitterWhole-cell responsesNervous systemCell-free patchesNervous tissueGABAA channelsRapid rundownDesensitization kineticsSingle-channel recordingsChannel currentsSingle-channel currentsExcised patch configurationsSingle-channel eventsUnitary conductance