1995
N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons
Bading H, Segal M, Sucher N, Dudek H, Lipton S, Greenberg M. N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons. Neuroscience 1995, 64: 653-664. PMID: 7715778, DOI: 10.1016/0306-4522(94)00462-e.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsBlotting, NorthernCalciumCells, CulturedElectrophysiologyGene Expression RegulationGenes, fosGenes, junGlutamic AcidGlyceraldehyde-3-Phosphate DehydrogenasesHippocampusImmunohistochemistryKainic AcidKynurenic AcidNeuronsNifedipineProto-Oncogene Proteins c-fosRatsRats, Inbred StrainsReceptors, N-Methyl-D-AspartateRNA, MessengerConceptsN-methyl-D-aspartate receptorsGene transcriptionCultured hippocampal neuronsD-aspartate receptorsRegulation of transcriptionHippocampal neuronsImmediate early gene transcriptionEarly gene transcriptionImmediate early genesTranscriptional regulationEarly gene expressionCalcium channelsTranscriptional responseCalcium imagingL-type voltage-sensitive calcium channelsN-methyl-D-aspartate (NMDA) subtypeExcitatory amino acid receptorsTranscriptional levelImmediate early gene expressionVoltage-sensitive calcium channelsNuclear runGene expressionL-type calcium channelsAmino acid receptorsRNA analysis
1991
Calcium channel antagonists and human immunodeficiency virus coat protein–mediated neuronal injury
Lipton S. Calcium channel antagonists and human immunodeficiency virus coat protein–mediated neuronal injury. Annals Of Neurology 1991, 30: 110-114. PMID: 1656845, DOI: 10.1002/ana.410300121.Peer-Reviewed Original ResearchConceptsVoltage-dependent calcium channelsCalcium channel antagonistsNeuronal injuryChannel antagonistsCalcium channelsHuman immunodeficiency virus type 1 (HIV-1) envelope proteinImmunodeficiency syndrome dementia complexRat retinal ganglion cellsSubsequent neuronal injuryNeuronal calcium channelsRetinal ganglion cellsVoltage-dependent Ca2Mammalian central neuronsChannel antagonist drugsNeuronal damageDementia complexMaximal doseCentral neuronsGanglion cellsDihydropyridine classIntracellular calciumAntagonist drugsElectrophysiological studiesLow micromolar concentrationsGp120Calcium channel antagonists attenuate NMDA receptor-mediated neurotoxicity of retinal ganglion cells in culture
Sucher N, Lei S, Lipton S. Calcium channel antagonists attenuate NMDA receptor-mediated neurotoxicity of retinal ganglion cells in culture. Brain Research 1991, 551: 297-302. PMID: 1680525, DOI: 10.1016/0006-8993(91)90944-q.Peer-Reviewed Original ResearchConceptsCalcium channel antagonistsRetinal ganglion cellsChannel antagonistsGanglion cellsPostnatal rat retinal ganglion cellsNMDA receptor-operated channelsRat retinal ganglion cellsNMDA receptor-mediated neurotoxicityVoltage-dependent calcium channelsSelective NMDA antagonistDihydropyridine calcium channel antagonistReceptor-operated channelsVoltage-dependent Ca2Patch-clamp recordingsNMDA antagonistsNMDA receptorsSubsequent toxic effectsCalcium channelsExogenous glutamateRetinal culturesAntagonistL-typeEarly riseInjurious effectsToxic effectsHIV‐Related Neurotoxicity
Lipton S. HIV‐Related Neurotoxicity. Brain Pathology 1991, 1: 193-199. PMID: 1669708, DOI: 10.1111/j.1750-3639.1991.tb00659.x.Peer-Reviewed Original ResearchConceptsNeuronal injuryCentral nervous system manifestationsL-type voltage-dependent calcium channelsVoltage-dependent calcium channelsNervous system manifestationsHIV-1 infectionToxic factorsWhite matter lesionsNeuronal cell deathIntracellular calcium concentrationNeuronal cell typesNeuronal lossSystem manifestationsMatter lesionsEndogenous glutamateImmune cellsGlutamate receptorsGp 120Human neuronsCalcium channelsGp120 fragmentRodent neuronsHIVGp120Potential neurotoxinsCalcium Channel Antagonists in the Prevention of Neurotoxicity
Lipton S. Calcium Channel Antagonists in the Prevention of Neurotoxicity. Advances In Pharmacology 1991, 22: 271-297. PMID: 1659865, DOI: 10.1016/s1054-3589(08)60038-1.Peer-Reviewed Original ResearchConceptsCalcium channel antagonistsCalcium channelsImportant calcium channelTypes of neurotoxicityPrevention of neurotoxicityPreliminary human studiesNeuroprotective agentsChannel antagonistsPharmacological antagonistsSystemic effectsHuman studiesPrimary neuronsVivo modelAntagonistNeurotoxicityChannel typesNeuronsPrevention
1989
Calcium channels in solitary retinal ganglion cells from post‐natal rat.
Karschin A, Lipton S. Calcium channels in solitary retinal ganglion cells from post‐natal rat. The Journal Of Physiology 1989, 418: 379-396. PMID: 2559971, PMCID: PMC1189978, DOI: 10.1113/jphysiol.1989.sp017847.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsWhole-cell recordingsCalcium currentGanglion cellsOmega-conotoxin fraction GVIAT-type calcium currentL-type calcium currentDorsal root ganglion neuronesCell-attached patch recordingsDihydropyridine agonist Bay K8644Agonist Bay K8644Central mammalian neuronesPost-natal ratsSingle-channel patch-clamp techniquesPatch-clamp techniqueCell-attached recordingsWhole-cell currentsNeuronal membrane potentialEquimolar Ba2Reversible blockadeBay K8644T-currentsGanglion neuronesPharmacological agentsCalcium channels