1997
Enhanced neuronal death from focal ischemia in AMPA-receptor transgenic mice
Le D, Das S, Wang Y, Yoshizawa T, Sasaki Y, Takasu M, Nemes A, Mendelsohn M, Dikkes P, Lipton S, Nakanishi N. Enhanced neuronal death from focal ischemia in AMPA-receptor transgenic mice. Brain Research 1997, 52: 235-241. PMID: 9495544, DOI: 10.1016/s0169-328x(97)00261-1.Peer-Reviewed Original ResearchMeSH KeywordsAlternative SplicingAnimalsBrainCell DeathCerebral CortexCrosses, GeneticDisease SusceptibilityFemaleGenetic VariationHeterozygoteIschemic Attack, TransientMaleMiceMice, Inbred C57BLMice, Inbred CBAMice, TransgenicNeocortexNeuronsPolymerase Chain ReactionReceptors, AMPATranscription, GeneticConceptsNeuronal cell deathMiddle cerebral arteryWild-type neuronsTransgenic miceAMPA receptorsCerebral ischemiaFocal ischemiaGluR2 flipExcitatory amino acid receptorsCell deathAcute cerebral ischemiaGlobal cerebral ischemiaAmino acid receptorsAMPAR-mediated currentsGlutamate receptor antagonistsPermanent focal ischemiaGenetic mouse modelsWild-type controlsExcitotoxic damageEAA receptorsLarge infarctionCerebral arteryFocal strokeGlutamate excitotoxicityNeuronal death
1995
N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons
Bading H, Segal M, Sucher N, Dudek H, Lipton S, Greenberg M. N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons. Neuroscience 1995, 64: 653-664. PMID: 7715778, DOI: 10.1016/0306-4522(94)00462-e.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsBlotting, NorthernCalciumCells, CulturedElectrophysiologyGene Expression RegulationGenes, fosGenes, junGlutamic AcidGlyceraldehyde-3-Phosphate DehydrogenasesHippocampusImmunohistochemistryKainic AcidKynurenic AcidNeuronsNifedipineProto-Oncogene Proteins c-fosRatsRats, Inbred StrainsReceptors, N-Methyl-D-AspartateRNA, MessengerConceptsN-methyl-D-aspartate receptorsGene transcriptionCultured hippocampal neuronsD-aspartate receptorsRegulation of transcriptionHippocampal neuronsImmediate early gene transcriptionEarly gene transcriptionImmediate early genesTranscriptional regulationEarly gene expressionCalcium channelsTranscriptional responseCalcium imagingL-type voltage-sensitive calcium channelsN-methyl-D-aspartate (NMDA) subtypeExcitatory amino acid receptorsTranscriptional levelImmediate early gene expressionVoltage-sensitive calcium channelsNuclear runGene expressionL-type calcium channelsAmino acid receptorsRNA analysis
1993
Prospects for clinically tolerated NMDA antagonists: open-channel blockers and alternative redox states of nitric oxide
Lipton S. Prospects for clinically tolerated NMDA antagonists: open-channel blockers and alternative redox states of nitric oxide. Trends In Neurosciences 1993, 16: 527-532. PMID: 7509524, DOI: 10.1016/0166-2236(93)90198-u.Peer-Reviewed Original ResearchConceptsExcitatory amino acid receptorsAmino acid receptorsAcid receptorsN-methyl-D-aspartate receptorsChronic neurological diseaseNMDA receptor antagonistNMDA receptor activityOpen channel blockOpen channel blockerReceptor antagonistNMDA antagonistsAnimal modelsNeurotoxic damageNeurological diseasesReceptor activityClinical useNitric oxideAntagonistReceptorsAlternative redox statesBlockersDisease
1988
The interaction of agonists and noncompetitive antagonists at the excitatory amino acid receptors in rat retinal ganglion cells in vitro
Karschin A, Aizenman E, Lipton. The interaction of agonists and noncompetitive antagonists at the excitatory amino acid receptors in rat retinal ganglion cells in vitro. Journal Of Neuroscience 1988, 8: 2895-2906. PMID: 2842467, PMCID: PMC6569411, DOI: 10.1523/jneurosci.08-08-02895.1988.Peer-Reviewed Original ResearchConceptsExcitatory amino acidsGanglion cellsMK-801Excitatory amino acid receptorsRat retinal ganglion cellsWhole-cell patch-clamp techniqueCultured ganglion cellsKainate-activated currentsKainate-induced currentsAmino acid receptorsConcentrations of NMDAApplication of kainateKainate-induced responsesPresence of NMDAReceptor-ion channel complexRetinal ganglion cellsNoncompetitive NMDA antagonistPatch-clamp techniqueDegree of blockAnticonvulsant MK-801Ganglion cell membraneDissociative anesthetic phencyclidineMicroM kainateKainate currentsLarge inward currentsResponses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat.
Aizenman E, Frosch M, Lipton S. Responses mediated by excitatory amino acid receptors in solitary retinal ganglion cells from rat. The Journal Of Physiology 1988, 396: 75-91. PMID: 2842491, PMCID: PMC1192034, DOI: 10.1113/jphysiol.1988.sp016951.Peer-Reviewed Original ResearchConceptsNon-desensitizing currentsDesensitizing componentGanglion cellsInward currentsExcitatory amino acid receptorsExcitatory amino acid responsesAgonist concentrationsWhole-cell voltage-clamp techniqueAmino acid receptorsL-glutamate responsesRetinal ganglion cellsHigh agonist concentrationsAmino acid responsesVoltage-clamp techniqueMicroM kynurenateQuisqualate responseMicroM kainateNMDA responsesNMDA currentsRat retinaAgonist doseQuisqualateAcid receptorsPharmacological propertiesAcid response