Characterization of angiotensin converting enzyme by [3H]captopril binding.
Strittmatter SM, Snyder SH. Characterization of angiotensin converting enzyme by [3H]captopril binding. Molecular Pharmacology 1986, 29: 142-8. PMID: 3005826.Peer-Reviewed Original ResearchMeSH KeywordsAngiotensin-Converting Enzyme InhibitorsAnimalsBinding SitesBromidesCaptoprilCobaltEnalaprilEnalaprilatKineticsPeptidyl-Dipeptidase ARatsSodium ChlorideThermodynamicsTritiumZincConceptsEnzyme-inhibitor interactionsActive site Zn2Dissociation rate constantsPure enzyme preparationCatalysisEnzyme moleculesNM-1 min-1Characterization of angiotensinRate constantsEnzyme preparationSubstrate catalysisSedimentation experimentsGel filtrationIonsMin-1Polypeptide chainBinding sitesIsomerizationMonophasic associationBindingZn2L-leucineMoleculesChloridePurification