2005
Reduced mitochondrial density and increased IRS-1 serine phosphorylation in muscle of insulin-resistant offspring of type 2 diabetic parents
Morino K, Petersen KF, Dufour S, Befroy D, Frattini J, Shatzkes N, Neschen S, White MF, Bilz S, Sono S, Pypaert M, Shulman GI. Reduced mitochondrial density and increased IRS-1 serine phosphorylation in muscle of insulin-resistant offspring of type 2 diabetic parents. Journal Of Clinical Investigation 2005, 115: 3587-3593. PMID: 16284649, PMCID: PMC1280967, DOI: 10.1172/jci25151.Peer-Reviewed Original ResearchMeSH KeywordsBiopsyBlood GlucoseBlotting, WesternBody Mass IndexBody WeightDiabetes Mellitus, Type 2DNA, MitochondrialFamily HealthFemaleGene Expression RegulationGlucose Clamp TechniqueGlucose Tolerance TestHumansHyperinsulinismImmunoprecipitationInsulinInsulin Receptor Substrate ProteinsInsulin ResistanceLipidsMaleMicroscopy, ElectronMicroscopy, Electron, TransmissionMitochondriaMusclesPhosphoproteinsPhosphorylationProtein Serine-Threonine KinasesReverse Transcriptase Polymerase Chain ReactionRNA, MessengerSerineSignal TransductionTime FactorsTranscription, GeneticTriglyceridesConceptsInsulin-resistant offspringIR offspringType 2 diabetesInsulin-stimulated muscle glucose uptakeType 2 diabetic parentsIntramyocellular lipid contentHyperinsulinemic-euglycemic clampMuscle glucose uptakeIRS-1 serine phosphorylationMuscle mitochondrial densityMitochondrial densityMuscle biopsy samplesSerine kinase cascadeInsulin-stimulated Akt activationDiabetic parentsInsulin resistanceControl subjectsBiopsy samplesGlucose uptakeLipid accumulationMitochondrial dysfunctionInsulin signalingAkt activationEarly defectsMuscle
1999
Search for variants of the gene-promoter and the potential phosphotyrosine encoding sequence of the insulin receptor substrate-2 gene: evaluation of their relation with alterations in insulin secretion and insulin sensitivity
Almind K, Frederiksen S, Bernal D, Hansen T, Ambye L, Urhammer S, Ekstrøm C, Berglund L, Reneland R, Lithell H, White M, Van Obberghen E, Pedersen O. Search for variants of the gene-promoter and the potential phosphotyrosine encoding sequence of the insulin receptor substrate-2 gene: evaluation of their relation with alterations in insulin secretion and insulin sensitivity. Diabetologia 1999, 42: 1244-1249. PMID: 10525667, DOI: 10.1007/s001250051299.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAmino Acid SubstitutionBase SequenceDiabetes Mellitus, Type 2Gene FrequencyGenetic Carrier ScreeningGenetic TestingGlucose Tolerance TestHumansInsulinInsulin Receptor Substrate ProteinsInsulin SecretionIntracellular Signaling Peptides and ProteinsMaleMiddle AgedMolecular Sequence DataPedigreePhosphoproteinsPhosphotyrosinePolymorphism, Single-Stranded ConformationalPromoter Regions, GeneticProspective StudiesTwo-Hybrid System TechniquesConceptsType II diabetic patientsII diabetic patientsSerum insulin concentrationsInsulin secretionDiabetic patientsInsulin sensitivityInsulin concentrationsGly1057Asp variantInsulin receptor substrate-2 (IRS-2) geneIntravenous glucose tolerance testGlucose-tolerant offspringGlucose tolerance testGlucose-tolerant subjectsIRS-2Elderly Swedish menMiddle-aged subjectsYoung healthy subjectsAmino acid variantsWildtype carriersDiabetic parentsTolerance testTolerant subjectsHealthy subjectsStudy groupInsulin sensivity