2022
The AIM2 inflammasome is activated in astrocytes during the late phase of EAE
Barclay WE, Aggarwal N, Deerhake ME, Inoue M, Nonaka T, Nozaki K, Luzum NA, Miao EA, Shinohara ML. The AIM2 inflammasome is activated in astrocytes during the late phase of EAE. JCI Insight 2022, 7: e155563. PMID: 35451371, PMCID: PMC9089781, DOI: 10.1172/jci.insight.155563.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisCentral nervous systemInflammasome activationInflammasome-mediated inflammationRole of inflammasomesApoptosis-associated speck-like proteinIL-1β releaseAIM2 inflammasome activationSpeck-like proteinAutoimmune encephalomyelitisEffector cytokinesAutoimmune conditionsIL-18Multiple sclerosisIL-1βDisease peakInflammatory responseSpinal cordMelanoma 2Mouse modelAnimal modelsReporter miceNervous systemMyeloid cellsAIM2 inflammasome
2021
Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease
Aggarwal N, Deerhake ME, DiPalma D, Shahi SK, Gaggioli MR, Mangalam AK, Shinohara ML. Secreted osteopontin from CD4+ T cells limits acute graft-versus-host disease. Cell Reports 2021, 37: 110170. PMID: 34965439, PMCID: PMC8759344, DOI: 10.1016/j.celrep.2021.110170.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsAcute GVHDHost diseaseMajor target organAcute graftBacteria AkkermansiaGastrointestinal pathologyMouse modelTarget organsFunction of osteopontinCD4Protective rolePotential biomarkersBeneficial effectsEpithelial cellsOPN isoformsMouse mutant modelsGVHDOsteopontinGraftCell deathDiseaseCellsMutant modelsAkkermansiaSingle-Cell Transcriptional Heterogeneity of Neutrophils During Acute Pulmonary Cryptococcus neoformans Infection
Deerhake ME, Reyes EY, Xu-Vanpala S, Shinohara ML. Single-Cell Transcriptional Heterogeneity of Neutrophils During Acute Pulmonary Cryptococcus neoformans Infection. Frontiers In Immunology 2021, 12: 670574. PMID: 33995406, PMCID: PMC8116703, DOI: 10.3389/fimmu.2021.670574.Peer-Reviewed Original ResearchConceptsPulmonary Cryptococcus neoformans infectionDistinct neutrophil subsetsCryptococcus neoformans infectionRole of neutrophilsLigand-receptor analysisCytokine gene expressionFirst-line defensePulmonary cryptococcosisNeoformans infectionDendritic cellsNeutrophil subsetsNeutrophil heterogeneityNeutrophil functionImmune cellsCell transcriptional heterogeneityAlveolar macrophagesNeutrophilsFungal infectionsCryptococcus neoformansInfectionSingle-cell transcriptional analysisGene expressionGenerate ROSOxidative stress signatureTranscriptional heterogeneity
2013
Inducible mouse models illuminate parameters influencing epigenetic inheritance
Wan M, Gu H, Wang J, Huang H, Zhao J, Kaundal RK, Yu M, Kushwaha R, Chaiyachati BH, Deerhake E, Chi T. Inducible mouse models illuminate parameters influencing epigenetic inheritance. Development 2013, 140: 843-852. PMID: 23325759, PMCID: PMC3557779, DOI: 10.1242/dev.088229.Peer-Reviewed Original ResearchConceptsTransgenerational inheritanceEpigenetic perturbationsModification patternsChromatin modification patternsRepressive chromatin modificationsAberrant epigenetic modificationsTarget gene sequenceMitotic inheritanceChromatin modificationsEpigenetic inheritanceEpigenetic stateMetastable epiallelesEpigenetic modificationsTranscription factorsGene sequencesDNA sequencesEpigenetic programmingTarget genesCOL1A1 locusFetal epigenomeExtraordinary malleabilityPleiotropic effectsInducible mouse modelEpigenomeTransient manipulation
2009
Genetic Analysis of Blood Pressure in 8 Mouse Intercross Populations
Feng M, Deerhake ME, Keating R, Thaisz J, Xu L, Tsaih SW, Smith R, Ishige T, Sugiyama F, Churchill GA, DiPetrillo K. Genetic Analysis of Blood Pressure in 8 Mouse Intercross Populations. Hypertension 2009, 54: 802-809. PMID: 19652078, PMCID: PMC2854560, DOI: 10.1161/hypertensionaha.109.134569.Peer-Reviewed Original ResearchConceptsQuantitative trait lociIntercross populationCausal genesBlood pressure quantitative trait lociExcellent genetic resourcesConserved roleQTL intervalsQTL analysisGenomic regionsMouse genomeTrait lociHuman genomeGenetic resourcesBioinformatics methodsGenetic basisCandidate genesGenetic analysisGenesMultiple crossesGenomeHypertension genesBlood pressureLociMiceBlood pressure regulation