2024
Multiple intravenous infusions versus a single infusion of mesenchymal stem cells in a rat model of cerebral ischemia.
Yokoyama T, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Ukai R, Oka S, Kocsis J, Honmou O. Multiple intravenous infusions versus a single infusion of mesenchymal stem cells in a rat model of cerebral ischemia. Journal Of Neurosurgery 2024, 1-9. PMID: 39454218, DOI: 10.3171/2024.6.jns241111.Peer-Reviewed Original ResearchInfusion of mesenchymal stem cellsMiddle cerebral artery occlusionMiddle cerebral artery occlusion inductionMesenchymal stem cellsCorpus callosumSystemic infusion of mesenchymal stem cellsMultiple infusionsAcute phaseMesenchymal stem cell infusionCerebral ischemiaStem cellsMultiple intravenous infusionsRat model of cerebral ischemiaHigh-dose infusionPermanent middle cerebral artery occlusionIschemic lesion volumeModels of cerebral ischemiaCC thicknessRandomized clinical trialsCerebral artery occlusionNeural tractsAcute cerebral strokeFunctional recovery outcomesMRI diffusion tensor imagingMSC-3Intravenous Infusion of Autologous Mesenchymal Stem Cells Expanded in Auto Serum for Chronic Spinal Cord Injury Patients: A Case Series
Hirota R, Sasaki M, Iyama S, Kurihara K, Fukushi R, Obara H, Oshigiri T, Morita T, Nakazaki M, Namioka T, Namioka A, Onodera R, Kataoka-Sasaki Y, Oka S, Takemura M, Ukai R, Yokoyama T, Sasaki Y, Yamashita T, Kobayashi M, Okuma Y, Kondo R, Aichi R, Ohmatsu S, Kawashima N, Ito Y, Kobune M, Takada K, Ishiai S, Ogata T, Teramoto A, Yamashita T, Kocsis J, Honmou O. Intravenous Infusion of Autologous Mesenchymal Stem Cells Expanded in Auto Serum for Chronic Spinal Cord Injury Patients: A Case Series. Journal Of Clinical Medicine 2024, 13: 6072. PMID: 39458022, PMCID: PMC11509003, DOI: 10.3390/jcm13206072.Peer-Reviewed Original ResearchAmerican Spinal Injury Association Impairment ScaleSevere spinal cord injuryMesenchymal stem cell infusionAutologous mesenchymal stem cellsIntravenous infusionCase seriesMesenchymal stem cellsQuality of lifeAuto-serumSpinal cord injuryFunctional improvementChronic spinal cord injury patientsIntravenous infusion of mesenchymal stem cellsInfusion of mesenchymal stem cellsStem cellsCord injurySpinal Cord Independence Measure IIIAmerican Spinal Injury Association Impairment Scale grades CSpinal cord injury patientsChronic SCI patientsClassification of Spinal Cord InjuryNeurological Classification of Spinal Cord InjurySignificant functional improvementAssociation Impairment ScaleInternational Standards for Neurological Classification of Spinal Cord InjuryIntravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients
Yamaki T, Oka S, Iyama S, Sasaki M, Onodera R, Kataoka-Sasaki Y, Namioka T, Namioka A, Nakazaki M, Takemura M, Ukai R, Yokoyama T, Sasaki Y, Yamashita T, Kobayashi M, Yamaguchi M, Fukino M, Takazawa T, Hayasaka M, Owaku T, Funakura M, Onodera S, Ito Y, Kobune M, Kato J, Ishiai S, Kocsis J, Odaki M, Iwadate Y, Kobayashi S, Honmou O. Intravenous infusion of auto-serum-expanded autologous mesenchymal stem cells into chronic severe brain injury patients. Interdisciplinary Neurosurgery 2024, 36: 101927. DOI: 10.1016/j.inat.2023.101927.Peer-Reviewed Original ResearchSevere brain injury patientsAutologous mesenchymal stem cellsBrain injury patientsIntravenous infusionMesenchymal stem cellsAdverse eventsInjury patientsInfused mesenchymal stem cellsHealth Stroke ScaleSerious adverse eventsFugl-Meyer AssessmentInitial case seriesBrain metabolic activityStem cellsMSC infusionStroke ScaleNeurological deteriorationBarthel IndexCase seriesCNS tumorsNeurological functionFunctional statusFunctional improvementChronic patientsPlacebo effectA surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats
Yasuda N, Sasaki M, Kocsis J, Kawaharada N, Honmou O. A surgical protocol for establishing spinal cord ischemia with extended lifespan and low complication rates in rats. World Neurosurgery 2024, 188: e349-e356. PMID: 38789035, DOI: 10.1016/j.wneu.2024.05.114.Peer-Reviewed Original ResearchIschemic spinal cord injurySpinal cord ischemiaCord ischemiaComplication rateRat modelTherapeutic strategiesEvaluate new therapeutic strategiesFunctional recoveryMale Sprague-Dawley ratsLow complication rateSprague-Dawley ratsSpecialized surgical equipmentExperimental animal modelsImprove functional recoveryPromote functional recoverySpinal cord injuryCross-clampingSevere neurological disordersAzygos veinSurgical protocolDescending aortaBulldog clampsLumbar levelsSpinal cordIschemic lesions
2023
Rehabilitation facilitates functional improvement following intravenous infusion of mesenchymal stem cells in the chronic phase of cerebral ischemia in rats
Yamashita T, Sasaki M, Sasaki Y, Nagahama H, Oka S, Kataoka-Sasaki Y, Ukai R, Yokoyama T, Kobayashi M, Kakizawa M, Kocsis J, Honmou O. Rehabilitation facilitates functional improvement following intravenous infusion of mesenchymal stem cells in the chronic phase of cerebral ischemia in rats. Brain Research 2023, 1825: 148709. PMID: 38072373, DOI: 10.1016/j.brainres.2023.148709.Peer-Reviewed Original ResearchRepeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury
Kurihara K, Sasaki M, Nagahama H, Obara H, Fukushi R, Hirota R, Yoshimoto M, Teramoto A, Kocsis J, Yamashita T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury. Brain Research 2023, 1817: 148484. PMID: 37442249, DOI: 10.1016/j.brainres.2023.148484.Peer-Reviewed Original ResearchConceptsSpinal cord injuryInfusion of MSCsChronic spinal cord injuryMesenchymal stem cellsAxonal sproutingCord injuryHD-MSCsIntravenous infusionMotor functionVehicle groupWeek 6Single high-dose infusionInjection of MSCsChronic SCI ratsSingle MSC injectionGreater functional recoveryRat SCI modelSingle intravenous infusionHigh-dose infusionLimited treatment optionsGreater functional improvementSingle high doseHigh disease burdenVivo diffusion tensor imagingEx vivo diffusion tensor imagingTherapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administrationHuman mesenchymal stem‐derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat
Nakazaki M, Lankford K, Yamamoto H, Mae Y, Kocsis J. Human mesenchymal stem‐derived extracellular vesicles improve body growth and motor function following severe spinal cord injury in rat. Clinical And Translational Medicine 2023, 13: e1284. PMID: 37323108, PMCID: PMC10272923, DOI: 10.1002/ctm2.1284.Peer-Reviewed Original ResearchConceptsSpinal cord injurySevere spinal cord injuryFunctional motor recoveryYoung adult ratsMotor recoveryMesenchymal stem/stromal cellsSmall extracellular vesiclesMSC-sEVsCord injuryM2 macrophagesMotor functionAdult ratsBody growthPro-inflammatory cytokine tumor necrosisAdult spinal cord injuryDay 7 post-SCISystemic pro-inflammatory cytokinesIGF-1 levelsPro-inflammatory cytokinesCytokine tumor necrosisSystemic serum levelsBroad therapeutic benefitsNormal body growthExtracellular vesiclesDifferent treatment groupsDeriving Schwann cells from hPSCs enables disease modeling and drug discovery for diabetic peripheral neuropathy
Majd H, Amin S, Ghazizadeh Z, Cesiulis A, Arroyo E, Lankford K, Majd A, Farahvashi S, Chemel A, Okoye M, Scantlen M, Tchieu J, Calder E, Le Rouzic V, Shibata B, Arab A, Goodarzi H, Pasternak G, Kocsis J, Chen S, Studer L, Fattahi F. Deriving Schwann cells from hPSCs enables disease modeling and drug discovery for diabetic peripheral neuropathy. Cell Stem Cell 2023, 30: 632-647.e10. PMID: 37146583, PMCID: PMC10249419, DOI: 10.1016/j.stem.2023.04.006.Peer-Reviewed Original ResearchConceptsDiabetic peripheral neuropathySchwann cellsPeripheral neuropathyPeripheral nervous systemPrimary Schwann cellsBupropion treatmentDiabetic patientsMyelin damageSensory dysfunctionPrimary gliaSelective vulnerabilityAntidepressant drugsHyperglycemic miceLower incidenceRetrospective analysisHuman pluripotent stem cellsSC deathNervous systemTherapeutic candidateHigh glucoseNeuropathyHealth recordsMolecular featuresStem cellsPluripotent stem cellsA practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats
Nagahama H, Sasaki M, Komatsu K, Sato K, Katagiri Y, Kamagata M, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Terada K, Kobayashi M, Kocsis J, Honmou O. A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats. Journal Of Neuroscience Methods 2023, 386: 109784. PMID: 36608904, DOI: 10.1016/j.jneumeth.2023.109784.Peer-Reviewed Original Research
2022
Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury
Hirota R, Sasaki M, Kataoka-Sasaki Y, Oshigiri T, Kurihara K, Fukushi R, Oka S, Ukai R, Yoshimoto M, Kocsis JD, Yamashita T, Honmou O. Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury. Journal Of Neurotrauma 2022, 39: 1665-1677. PMID: 35611987, PMCID: PMC9734021, DOI: 10.1089/neu.2022.0106.Peer-Reviewed Original ResearchConceptsSpinal cord injuryCorticospinal tractMesenchymal stem cellsCord injurySpinal cordSpontaneous recoveryInfused mesenchymal stem cellsLimited spontaneous recoveryDorsal corticospinal tractLateral corticospinal tractStem cellsCST pathwayCST projectionsSCI inductionMSC infusionAxonal sproutingFunctional recoveryLateral funiculusIntravenous infusionAxonal tracerLesion coreMotor pathwaysFunctional improvementCircuit reorganizationMajor projectionsIntravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial
Oka S, Yamaki T, Sasaki M, Ukai R, Takemura M, Yokoyama T, Kataoka-Sasaki Y, Onodera R, Ito YM, Kobayashi S, Kocsis JD, Iwadate Y, Honmou O. Intravenous Infusion of Autoserum-Expanded Autologous Mesenchymal Stem Cells in Patients With Chronic Brain Injury: Protocol for a Phase 2 Trial. JMIR Research Protocols 2022, 11: e37898. PMID: 35793128, PMCID: PMC9301565, DOI: 10.2196/37898.Peer-Reviewed Original ResearchChronic brain injuryAutologous mesenchymal stem cellsSpinal cord injuryINTERNATIONAL REGISTERED REPORT IDENTIFIERBrain injuryIntravenous infusionRankin Scale gradesCord injuryMesenchymal stem cellsClinical trialsScale gradeTherapeutic efficacyJapan Medical Association CenterSapporo Medical University HospitalInvestigator-initiated clinical trialsModified Rankin Scale gradesOpen-label trialPhase 2 studyPhase 2 trialMedical University HospitalCause of disabilityMotor vehicle accidentsPotential therapeutic efficacyProportion of casesStem cells
2021
Repeated intravenous infusion of mesenchymal stem cells for enhanced functional recovery in a rat model of chronic cerebral ischemia.
Takemura M, Sasaki M, Kataoka-Sasaki Y, Kiyose R, Nagahama H, Oka S, Ukai R, Yokoyama T, Kocsis J, Ueba T, Honmou O. Repeated intravenous infusion of mesenchymal stem cells for enhanced functional recovery in a rat model of chronic cerebral ischemia. Journal Of Neurosurgery 2021, 137: 402-411. PMID: 34861644, DOI: 10.3171/2021.8.jns21687.Peer-Reviewed Original ResearchMiddle cerebral artery occlusionInduction of MCAOCorpus callosumMesenchymal stem cellsChronic phaseMotor functionRat modelWeek 8Vehicle groupInterhemispheric connectionsAdministration of MSCsChronic cerebral ischemia modelNeural connectionsChronic stroke modelCerebral artery occlusionChronic cerebral ischemiaIschemic lesion volumeLong-term disabilityGreater functional improvementCerebral ischemia modelEnhanced functional recoveryAnti-neurofilament antibodiesDiffusion tensorSingle systemic infusionStem cellsPossible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat
Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O. Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat. BMC Urology 2021, 21: 156. PMID: 34774029, PMCID: PMC8590770, DOI: 10.1186/s12894-021-00923-3.Peer-Reviewed Original ResearchConceptsHunner-type ICBladder pain syndromeAnti-inflammatory pathwayMSC groupMesenchymal stem cellsPain syndromeAnimal modelsToll-like receptor 7 agonistTherapeutic efficacyBehavior testsRight external jugular veinFemale Sprague-Dawley ratsGFP-positive mesenchymal stem cellsReceptor 7 agonistChronic pelvic painAnti-inflammatory effectsEfficacious treatment optionExperimental animal modelsSprague-Dawley ratsExternal jugular veinReal-time polymerase chain reactionMRNA expression levelsPositive mesenchymal stem cellsStem cellsHunner lesions
2014
Transplantation of Schwann cells and olfactory ensheathing cells as a therapeutic strategy in spinal cord injury
Kocsis J, Bunge M. Transplantation of Schwann cells and olfactory ensheathing cells as a therapeutic strategy in spinal cord injury. 2014, 496-513. DOI: 10.1017/cbo9780511995583.036.Peer-Reviewed Original ResearchNervous systemNeural repairNormal central nervous systemSpinal cord damageSpinal cord injuryRecovery of functionCentral nervous systemNeuron replacementCerebral palsyCord damageCord injuryAxonal regenerationNeuronal deathSchwann cellsTherapeutic strategiesAxon regenerationNeurological rehabilitationBrain disordersCell therapyRehabilitation professionalsInjuryRepairRehabilitationBasic scienceStem cell biology
2013
Sural nerve defects after nerve biopsy or nerve transfer as a sensory regeneration model for peripheral nerve conduit implantation
Radtke C, Kocsis J, Reimers K, Allmeling C, Vogt P. Sural nerve defects after nerve biopsy or nerve transfer as a sensory regeneration model for peripheral nerve conduit implantation. Medical Hypotheses 2013, 81: 500-502. PMID: 23867139, DOI: 10.1016/j.mehy.2013.06.020.Peer-Reviewed Original ResearchConceptsConduit implantationNerve repairNerve biopsyNerve injurySural nerveAxonal regenerationNerve defectsAcute peripheral nerve injuryHuman nerve injurySural nerve graftRecovery of sensationPeripheral nerve injuryVon Frey filamentsExtent of injuryNerve graft harvestingCold allodyniaMajor morbidityNerve transferNerve stumpNerve graftsNeuroma formationNerve lengthDigital nerveGraft harvestingAutograft treatmentSciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat
Lankford K, Arroyo E, Liu C, Somps C, Zorbas M, Shelton D, Evans M, Hurst S, Kocsis J. Sciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat. Neuroscience 2013, 241: 157-169. PMID: 23531437, DOI: 10.1016/j.neuroscience.2013.03.024.Peer-Reviewed Original ResearchConceptsNerve growth factorAdult ratsNerve regenerationFunctional recoveryAnti-NGF antibody treatmentElevated nerve growth factorUnilateral sciatic nerve crushDorsal root ganglion neuronsAnti-NGF antibodySciatic nerve crushType of painVehicle-treated animalsSciatic nerve regenerationPost nerve injuryNovel therapeutic approachesCell body sizePeripheral nerve regenerationFluro-GoldPeripheral nervous system developmentNerve injuryPain modelNerve crushPain managementAntibody treatmentGait recoveryParacrine Loop of Keratinocyte Proliferation and Directed Neuritic Outgrowth in a Neuroepithelial Coculture
Radtke C, Rennekampff H, Reimers K, Vogt P, Kocsis J. Paracrine Loop of Keratinocyte Proliferation and Directed Neuritic Outgrowth in a Neuroepithelial Coculture. Annals Of Plastic Surgery 2013, Publish Ahead of Print: &na;. DOI: 10.1097/sap.0b013e318276d946.Peer-Reviewed Original ResearchNerve growth factorNeuritic outgrowthDRG neuronsHuman keratinocytesNeurotrophin nerve growth factorDorsal root ganglion neuronsCutaneous afferent axonsKeratinocyte proliferationAnti-NGF antibodyWound healingChronic nonhealing woundsProliferation of keratinocytesNGF levelsSkin innervationPrimary human keratinocytesNeurotrophic factorGanglion neuronsAfferent axonsParacrine loopNonhealing woundsMitogenic effectCell bodiesNeuritic processesGrowth factorNeurons
2012
LOP08
Radtke C, Lankford K, Sasaki M, Kocsis J, Vogt P. LOP08. Plastic & Reconstructive Surgery 2012, 130: 481. DOI: 10.1097/01.prs.0000418400.18580.18.Peer-Reviewed Original Research
2011
LOP33: KERATINOCYTES INDUCE EXTREME SENSORY NEURONAL HYPEREXCITABILITY AND CHRONIC PAIN
Radtke C, Kocsis J, Vogt P. LOP33: KERATINOCYTES INDUCE EXTREME SENSORY NEURONAL HYPEREXCITABILITY AND CHRONIC PAIN. Plastic & Reconstructive Surgery 2011, 128: 626. DOI: 10.1097/01.prs.0000400386.71166.48.Peer-Reviewed Original Research