2023
Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury
Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis J, Honmou O. Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury. Pediatric Research 2023, 94: 1921-1928. PMID: 37422495, DOI: 10.1038/s41390-023-02717-9.Peer-Reviewed Original ResearchConceptsPerinatal brain injuryBrain injuryMesenchymal stem cellsIntravenous infusionVehicle groupBrain volumeTherapeutic efficacyInfused mesenchymal stem cellsLeft common carotid arteryHistological analysisNon-ischemic hemispherePostnatal day 7Common carotid arteryEmbryonic day 18Stem cellsHypoxia-ischemiaMSC infusionPreterm infantsGABAergic cellsNeurological functionSignificant complicationsCortical synapsesFunctional improvementCarotid arteryIntravenous administration
2021
Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat
Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O. Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat. BMC Urology 2021, 21: 156. PMID: 34774029, PMCID: PMC8590770, DOI: 10.1186/s12894-021-00923-3.Peer-Reviewed Original ResearchConceptsHunner-type ICBladder pain syndromeAnti-inflammatory pathwayMSC groupMesenchymal stem cellsPain syndromeAnimal modelsToll-like receptor 7 agonistTherapeutic efficacyBehavior testsRight external jugular veinFemale Sprague-Dawley ratsGFP-positive mesenchymal stem cellsReceptor 7 agonistChronic pelvic painAnti-inflammatory effectsEfficacious treatment optionExperimental animal modelsSprague-Dawley ratsExternal jugular veinReal-time polymerase chain reactionMRNA expression levelsPositive mesenchymal stem cellsStem cellsHunner lesions
2013
Sciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat
Lankford K, Arroyo E, Liu C, Somps C, Zorbas M, Shelton D, Evans M, Hurst S, Kocsis J. Sciatic nerve regeneration is not inhibited by anti-NGF antibody treatment in the adult rat. Neuroscience 2013, 241: 157-169. PMID: 23531437, DOI: 10.1016/j.neuroscience.2013.03.024.Peer-Reviewed Original ResearchConceptsNerve growth factorAdult ratsNerve regenerationFunctional recoveryAnti-NGF antibody treatmentElevated nerve growth factorUnilateral sciatic nerve crushDorsal root ganglion neuronsAnti-NGF antibodySciatic nerve crushType of painVehicle-treated animalsSciatic nerve regenerationPost nerve injuryNovel therapeutic approachesCell body sizePeripheral nerve regenerationFluro-GoldPeripheral nervous system developmentNerve injuryPain modelNerve crushPain managementAntibody treatmentGait recovery
2009
Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells
Radtke C, Schmitz B, Spies M, Kocsis J, Vogt P. Peripheral glial cell differentiation from neurospheres derived from adipose mesenchymal stem cells. International Journal Of Developmental Neuroscience 2009, 27: 817-823. PMID: 19699793, DOI: 10.1016/j.ijdevneu.2009.08.006.Peer-Reviewed Original ResearchConceptsMesenchymal stem cellsStem cellsGlial-like cellsAdipose-derived mesenchymal stem cellsGlial cell differentiationPeripheral glial cellsGrowth factorEpidermal growth factorGrowth factor receptorMitogen withdrawalFibroblast growth factorBasic fibroblast growth factorCell differentiationDorsal root ganglion neuronsSchwann cell marker S100P75 nerve growth factor receptorAdipose-derived stem cellsNerve growth factor receptorCellular aggregatesSimultaneous expressionOlfactory Ensheathing CellsAppropriate inductionFactor receptorNeurospheresGlia markers
2006
Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells
Dombrowski MA, Sasaki M, Lankford KL, Kocsis JD, Radtke C. Myelination and nodal formation of regenerated peripheral nerve fibers following transplantation of acutely prepared olfactory ensheathing cells. Brain Research 2006, 1125: 1-8. PMID: 17112480, PMCID: PMC2673087, DOI: 10.1016/j.brainres.2006.09.089.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, Genetically ModifiedCell Adhesion Molecules, NeuronalCell TransplantationGreen Fluorescent ProteinsImmunohistochemistryMicroscopy, ImmunoelectronMyelin SheathNAV1.6 Voltage-Gated Sodium ChannelNerve RegenerationNeurofilament ProteinsNeurogliaOlfactory BulbRanvier's NodesRatsRats, Sprague-DawleySciatic NeuropathySodium ChannelsTime FactorsConceptsPeripheral nerve fibersPeripheral nervesNodes of RanvierFunctional outcomeAxonal regenerationNerve fibersRegenerated peripheral nerve fibersSciatic nerve crush lesionNerve crush lesionPeripheral-type myelinSpinal cord resultsTransplantation of olfactoryPeripheral axonal regenerationParanodal CasprCrush lesionCord resultsFunctional improvementOlfactory bulbTransection siteTransgenic ratsLesion zoneNerveNodal formationTransplantation siteOECsNeuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia
Liu H, Honmou O, Harada K, Nakamura K, Houkin K, Hamada H, Kocsis J. Neuroprotection by PlGF gene-modified human mesenchymal stem cells after cerebral ischaemia. Brain 2006, 129: 2734-2745. PMID: 16901914, PMCID: PMC2605397, DOI: 10.1093/brain/awl207.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsApoptosisBrain IschemiaGenetic VectorsHumansImage Processing, Computer-AssistedInfarction, Middle Cerebral ArteryMagnetic Resonance ImagingMaleMesenchymal Stem Cell TransplantationMesenchymal Stem CellsModels, AnimalNeovascularization, PathologicNeuropsychological TestsPlacenta Growth FactorPregnancy ProteinsRatsRats, Sprague-DawleyTransduction, GeneticConceptsMiddle cerebral artery occlusionCerebral ischaemiaMesenchymal stem cellsIntravenous deliveryPermanent middle cerebral artery occlusionHuman MSCsControl sham groupLimb placement testRats 3 hCerebral artery occlusionTreadmill stress testPlacental growth factorIntraluminal vascular occlusionEnzyme-linked immunosorbentNon-neural tissuesHuman mesenchymal stem cellsStem cellsAdult bone marrowArtery occlusionInfarcted hemisphereSham groupFunctional outcomeVascular occlusionFunctional deficitsInfarction size
2005
I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Nomura T, Honmou O, Harada K, Houkin K, Hamada H, Kocsis J. I.v. infusion of brain-derived neurotrophic factor gene-modified human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Neuroscience 2005, 136: 161-169. PMID: 16229956, PMCID: PMC2605391, DOI: 10.1016/j.neuroscience.2005.06.062.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorMesenchymal stem cell groupBrain-derived neurotrophic factor contributesMiddle cerebral artery occlusionStem cell groupCerebral artery occlusionCerebral ischemia modelMesenchymal stem cellsNeurotrophic factorArtery occlusionCell groupsIschemia modelPermanent middle cerebral artery occlusionRat cerebral ischemia modelHuman mesenchymal stem cellsBrain-derived neurotrophic factor (BDNF) geneStem cellsControl sham groupTreadmill stress testRats 6 hFactor contributesNeurotrophic factor geneIntraluminal vascular occlusionAdult bone marrowCerebral ischemiaIntravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat
Honma T, Honmou O, Iihoshi S, Harada K, Houkin K, Hamada H, Kocsis J. Intravenous infusion of immortalized human mesenchymal stem cells protects against injury in a cerebral ischemia model in adult rat. Experimental Neurology 2005, 199: 56-66. PMID: 15967439, PMCID: PMC2605388, DOI: 10.1016/j.expneurol.2005.05.004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntigens, CDBehavior, AnimalBrain IschemiaCell CountCells, CulturedDisease Models, AnimalExercise TestGreen Fluorescent ProteinsHumansImmunohistochemistryInfusions, IntravenousMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleMaze LearningMesenchymal Stem Cell TransplantationMesenchymal Stem CellsPhosphopyruvate HydrataseRatsRats, Sprague-DawleyTime FactorsConceptsMiddle cerebral artery occlusionIntravenous infusionFunctional outcomeLesion sizeTransient middle cerebral artery occlusionMesenchymal stem cellsCerebral artery occlusionCerebral infarction volumeCerebral ischemia modelSpinal cord injurySubsequent histological examinationPotential therapeutic benefitRats 12 hMorris water mazeBone marrow cellsHuman mesenchymal stem cellsStem cellsHTERT-MSCsArtery occlusionInfarction volumeCerebral ischemiaCord injuryFunctional improvementLesion volumeIschemia model
2000
Synaptic Reorganization in the Substantia Gelatinosa After Peripheral Nerve Neuroma Formation: Aberrant Innervation of Lamina II Neurons by Aβ Afferents
Kohama I, Ishikawa K, Kocsis J. Synaptic Reorganization in the Substantia Gelatinosa After Peripheral Nerve Neuroma Formation: Aberrant Innervation of Lamina II Neurons by Aβ Afferents. Journal Of Neuroscience 2000, 20: 1538-1549. PMID: 10662843, PMCID: PMC2605372, DOI: 10.1523/jneurosci.20-04-01538.2000.Peer-Reviewed Original ResearchConceptsLamina II neuronsSciatic nerve sectionLaminae IIINeuroma formationNerve sectionLamina IIDorsal hornNerve stimulationMonosynaptic EPSPsAbeta fibersControl slicesSpinal cord dorsal horn neuronsExtracellular field potential recordingsLow-threshold stimulationDorsal horn neuronsDorsal root axonsLow-threshold mechanoreceptorsSpinal cord slicesShort-latency responsesTransverse slice preparationHorseradish peroxidase labelingField potential recordingsLaminae IAberrant innervationSubstantia gelatinosa
1996
The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission
Zahler R, Sun W, Ardito T, Zhang Z, Kocsis J, Kashgarian M. The α3 Isoform Protein of the Na+,K+-ATPase Is Associated With the Sites of Cardiac and Neuromuscular Impulse Transmission. Circulation Research 1996, 78: 870-879. PMID: 8620608, DOI: 10.1161/01.res.78.5.870.Peer-Reviewed Original ResearchConceptsNeuromuscular junctionConduction systemImpulse transmissionIsoform-specific antibodiesSkeletal muscle expressesSkeletal musclePresynaptic motor terminalsCardiac conduction systemHeart conduction systemAlpha subunitAxonal processesRat heartCardiac myocytesIsoform mRNAsMuscleClassic morphologyJunctional complexesImmunoreactive proteinPump isoformsAntibodiesIsoform proteinsHeartPump proteinImmunohistochemistryIsoforms
1993
Transient presence of GABA in astrocytes of the developing optic nerve
Ochi S, Lim J, Rand M, During M, Sakatani K, Kocsis J. Transient presence of GABA in astrocytes of the developing optic nerve. Glia 1993, 9: 188-198. PMID: 8294149, DOI: 10.1002/glia.440090304.Peer-Reviewed Original ResearchConceptsWhole optic nerveOptic nerveHigh-pressure liquid chromatographyGABA immunoreactivityPostnatal developmentEnzyme glutamic acid decarboxylaseIntact optic nerveRat optic nerveNeonatal optic nerveGlutamic acid decarboxylaseAstrocyte cell bodiesPostnatal day 20GABA immunostainingGFAP stainingPostnatal weekGABA stainingNerveCultured astrocytesAstrocytesDay 20GABAAcid decarboxylaseCell bodiesDevelopmental time courseImmunoreactivity