Featured Publications
Stress primes microglial polarization after global ischemia: Therapeutic potential of progesterone
Espinosa-Garcia C, Sayeed I, Yousuf S, Atif F, Sergeeva E, Neigh G, Stein D. Stress primes microglial polarization after global ischemia: Therapeutic potential of progesterone. Brain Behavior And Immunity 2017, 66: 177-192. PMID: 28648389, DOI: 10.1016/j.bbi.2017.06.012.Peer-Reviewed Original ResearchConceptsAnti-inflammatory cytokinesGlobal ischemiaDays of social defeat stressIncreased pro-inflammatory cytokine expressionPost-ischemiaIntraperitoneal injections of progesteroneNeuroprotective effects of progesteroneMale Sprague-Dawley ratsIschemic strokeAssociated with increased riskMicroglial polarizationSocial defeat stressCA1 hippocampal regionEffects of progesteroneAssociated with increased risk of strokePro-inflammatory cytokine expressionSprague-Dawley ratsNeurotrophic factor expressionInjection of progesteroneAnti-inflammatory mediatorsPro-inflammatory cytokinesExacerbate neuronal lossRisk of strokeM2 phenotype markersPotential therapeutic strategy
2020
Stroke-Induced Peripheral Immune Dysfunction in Vitamin D–Deficient Conditions: Modulation by Progesterone and Vitamin D
Yousuf S, Atif F, Espinosa-Garcia C, Harris W, Turan N, Stein D. Stroke-Induced Peripheral Immune Dysfunction in Vitamin D–Deficient Conditions: Modulation by Progesterone and Vitamin D. Molecular Neurobiology 2020, 58: 950-963. PMID: 33063282, DOI: 10.1007/s12035-020-02129-4.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsApoptosisAstrocytesBehavior, AnimalBiomarkersCyclooxygenase 2Endoplasmic Reticulum StressHand StrengthInflammationInflammation MediatorsInterleukin-6LipopolysaccharidesMaleMyelin Basic ProteinNeuronsNitric Oxide Synthase Type IIProgesteroneRatsRats, Sprague-DawleyReactive Oxygen SpeciesReproducibility of ResultsSpleenStrokeThymus GlandTranscription Factor CHOPVitamin DVitamin D DeficiencyWhite MatterConceptsPeripheral immune dysfunctionSystemic inflammationImmune dysfunctionCombined treatmentLipopolysaccharide (LPS)-induced systemic inflammationVitamin D. Vitamin D deficiencyImmune cell populationsAdministration of progesteroneVitamin D-deficient conditionDelayed functional recoveryD sufficiencyD deficiencyEndoplasmic reticulum stressTreated with PVitamin DWhite matter integrityComorbid conditionsDay 4Improve outcomesFunctional recoveryAltered outcomesInflammationNeuronal inflammationCell populationsReticulum stressPost-ischemic stroke systemic inflammation: Immunomodulation by progesterone and vitamin D hormone
Atif F, Yousuf S, Espinosa-Garcia C, Harris W, Stein D. Post-ischemic stroke systemic inflammation: Immunomodulation by progesterone and vitamin D hormone. Neuropharmacology 2020, 181: 108327. PMID: 32950558, DOI: 10.1016/j.neuropharm.2020.108327.Peer-Reviewed Original ResearchConceptsPeripheral immune dysfunctionVitamin D hormoneTransient middle cerebral artery occlusion/reperfusionImmune dysfunctionSystemic inflammationInfarct volumeSubsets of immune cellsNeuronal inflammationMeasure infarct volumePost-stroke infectionsInjection of lipopolysaccharideIn-hospital infectionStandard of careFlow cytometric analysisMiddle cerebral artery occlusion/reperfusionCleaved caspase-3Combination therapyInflammation groupImmune cellsAdult ratsIncreased morbidityD hormoneBrain post-strokeImmunomodulatory effectsCombined treatment
2016
Disruption of motor behavior and injury to the CNS induced by 3-thienylboronic acid in mice
Farfán-García E, Pérez-Rodríguez M, Espinosa-García C, Castillo-Mendieta N, Maldonado-Castro M, Querejeta E, Trujillo-Ferrara J, Soriano-Ursúa M. Disruption of motor behavior and injury to the CNS induced by 3-thienylboronic acid in mice. Toxicology And Applied Pharmacology 2016, 307: 130-137. PMID: 27495897, DOI: 10.1016/j.taap.2016.08.002.Peer-Reviewed Original ResearchConceptsCentral nervous systemDisruption of motor activityDose of 400mg/kgMotor disruptionLoss of neuronsCD1 miceIntraperitoneal administrationMotor behaviorMotor activitySubstantia nigraNervous systemDoseMiceMotor performanceMotor systemCell damageMotor cortexNeurotoxic agentsInjuryBoron-free compoundsEffects of BCCAdministrationScarcity of studies