Featured Publications
Stress primes microglial polarization after global ischemia: Therapeutic potential of progesterone
Espinosa-Garcia C, Sayeed I, Yousuf S, Atif F, Sergeeva E, Neigh G, Stein D. Stress primes microglial polarization after global ischemia: Therapeutic potential of progesterone. Brain Behavior And Immunity 2017, 66: 177-192. PMID: 28648389, DOI: 10.1016/j.bbi.2017.06.012.Peer-Reviewed Original ResearchConceptsAnti-inflammatory cytokinesGlobal ischemiaDays of social defeat stressIncreased pro-inflammatory cytokine expressionPost-ischemiaIntraperitoneal injections of progesteroneNeuroprotective effects of progesteroneMale Sprague-Dawley ratsIschemic strokeAssociated with increased riskMicroglial polarizationSocial defeat stressCA1 hippocampal regionEffects of progesteroneAssociated with increased risk of strokePro-inflammatory cytokine expressionSprague-Dawley ratsNeurotrophic factor expressionInjection of progesteroneAnti-inflammatory mediatorsPro-inflammatory cytokinesExacerbate neuronal lossRisk of strokeM2 phenotype markersPotential therapeutic strategy
2020
Peripheral Myeloid Cell EP2 Activation Contributes to the Deleterious Consequences of Status Epilepticus
Varvel N, Espinosa-Garcia C, Hunter-Chang S, Chen D, Biegel A, Hsieh A, Blackmer-Raynolds L, Ganesh T, Dingledine R. Peripheral Myeloid Cell EP2 Activation Contributes to the Deleterious Consequences of Status Epilepticus. Journal Of Neuroscience 2020, 41: 1105-1117. PMID: 33293358, PMCID: PMC7880294, DOI: 10.1523/jneurosci.2040-20.2020.Peer-Reviewed Original ResearchConceptsBlood-brain barrierImmune myeloid cellsEP2 receptorInnate immune cellsStatus epilepticusEP2 antagonismProstaglandin EP2 receptorBlood-borne monocytesMyeloid cellsImmune cellsConsequences of seizuresBrain infiltrationInflammatory cytokinesFormation of inflammatory cytokinesConditional KOInflammatory responsePeripheral innate immune cellsActivation of EP2 receptorsQuality of lifeEP2 receptor antagonistRecruitment of blood-borne monocytesSerum albumin levelPeripheral immune cellsConsequences of status epilepticusHippocampal IL-6Progesterone Attenuates Stress-Induced NLRP3 Inflammasome Activation and Enhances Autophagy Following Ischemic Brain Injury
Espinosa-Garcia C, Atif F, Yousuf S, Sayeed I, Neigh G, Stein D. Progesterone Attenuates Stress-Induced NLRP3 Inflammasome Activation and Enhances Autophagy Following Ischemic Brain Injury. International Journal Of Molecular Sciences 2020, 21: 3740. PMID: 32466385, PMCID: PMC7312827, DOI: 10.3390/ijms21113740.Peer-Reviewed Original ResearchConceptsHigh-mobility group box 1HMGB1 releaseIschemic brain injuryNLRP3 inflammasome activationExposed to social defeat stressGlobal ischemiaIschemic animalsAssociated with cardiac arrestInflammasome activationAdult male Sprague-Dawley ratsMale Sprague-Dawley ratsNeonatal brain tissueHippocampus of ischemic animalsRodent models of cerebral ischemiaSocial defeat stressRelease of alarminsModels of cerebral ischemiaSprague-Dawley ratsBrain injuryNOD-like receptor pyrin domainNLRP3 inflammasomeResponse to ischemiaPrimed with lipopolysaccharideBlocks progesteroneDays post-ischemia
2013
Effects of progesterone on neurite growth inhibitors in the hippocampus following global cerebral ischemia
Espinosa-García C, Aguilar-Hernández A, Cervantes M, Moralí G. Effects of progesterone on neurite growth inhibitors in the hippocampus following global cerebral ischemia. Brain Research 2013, 1545: 23-34. PMID: 24316245, DOI: 10.1016/j.brainres.2013.11.030.Peer-Reviewed Original ResearchConceptsEffects of progesteroneGlobal cerebral ischemiaMorris water mazeCA1 pyramidal neuronsNogo-ACerebral ischemiaPyramidal neuronsRho ACell bodies of CA1 pyramidal neuronsNeurite growth inhibitor Nogo-ASpatial learningVehicle-treated ratsAdult male ratsTreated with P4Effects of P4Dendritic arborization of CA1 pyramidal neuronsAssociated with parametersNeurite growth inhibitorsFunction preservationMale ratsRat hippocampusWater mazeIschemiaHippocampal layersRatsPost-ischemic administration of progesterone reduces caspase-3 activation and DNA fragmentation in the hippocampus following global cerebral ischemia
Espinosa-García C, Vigueras-Villaseñor R, Rojas-Castañeda J, Aguilar-Hernández A, Monfil T, Cervantes M, Moralí G. Post-ischemic administration of progesterone reduces caspase-3 activation and DNA fragmentation in the hippocampus following global cerebral ischemia. Neuroscience Letters 2013, 550: 98-103. PMID: 23810799, DOI: 10.1016/j.neulet.2013.06.023.Peer-Reviewed Original ResearchConceptsCaspase-3 levelsGlobal cerebral ischemia/reperfusionCaspase-3Pyramidal neuronsCaspase-3 activityAdult male Sprague-Dawley ratsHippocampal CA1 pyramidal neuronsNeuroprotective effects of progesteroneCerebral ischemia/reperfusionMale Sprague-Dawley ratsGlobal cerebral ischemia/reperfusion injuryCA1 pyramidal neuronsNeuroprotective effectsEffects of progesteroneAdministration of progesteroneIschemia/reperfusion-induced increasesSprague-Dawley ratsFour-vessel occlusion modelModels of brain injuryEffects of P4CA1 neuronal lossGlobal cerebral ischemiaPost-ischemic administrationCerebral ischemia/reperfusion injuryDNA fragmentation