2024
Proximity Labeling Proteomics Reveals Kv1.3 Potassium Channel Immune Interactors in Microglia
Bowen C, Nguyen H, Lin Y, Bagchi P, Natu A, Espinosa-Garcia C, Werner E, Kumari R, Brandelli A, Kumar P, Tobin B, Wood L, Faundez V, Wulff H, Seyfried N, Rangaraju S. Proximity Labeling Proteomics Reveals Kv1.3 Potassium Channel Immune Interactors in Microglia. Molecular & Cellular Proteomics 2024, 23: 100809. PMID: 38936775, DOI: 10.1016/j.mcpro.2024.100809.Peer-Reviewed Original ResearchDisease-associated microgliaPDZ-binding domainImmune signaling proteinsInflammatory responseProinflammatory signaling moleculesInhibition of Kv1.3Resident immune cellsSignaling proteinsKv1.3 potassium channelsC-terminusC-terminal PDZ-binding domainMicroglia transitionTLR4-mediated activationLipopolysaccharide-induced inflammatory responseKv1.3 blockadeImmune cellsPotential interacting proteinsInflammatory statePotassium channelsInflammatory contextKv1.3Flux of potassium ionsTLR4-induced inflammatory responsesFlow cytometryGlycolytic response
2023
Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease
Bowen C, Lin Y, Zeng H, Nguyen H, Xiao H, Kumar P, Duong D, Espinosa‐Garcia C, Wulff H, Seyfried N, Rangaraju S. Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078851.Peer-Reviewed Original ResearchKv1.3 blockadeKv1.3 channelsKv1.3 blockersMouse model of AD pathologyBlockade of Kv1.3Immune regulatory roleWestern blottingAlzheimer's diseaseCMV-Cre miceChannels in vitroPDZ-binding motifPlasma membrane proteinsInhibition in vivoCell type-specificPharmacological blockadeOlder 5XFAD miceCMV-CreFunction of microgliaChannel abundanceKv1.3Mouse modelBiotin ligaseFlow cytometryTrafficking proteinsBiotinylated proteins