Claudia Espinosa-Garcia
she/her/hers
Associate Research ScientistCards
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Research
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Featured Publications
Native-state proteomics of Parvalbumin interneurons identifies novel molecular signatures and metabolic vulnerabilities to early Alzheimer's disease pathology.
Kumar P, Goettemoeller AM, Espinosa-Garcia C, Tobin BR, Tfaily A, Nelson RS, Natu A, Dammer EB, Santiago JV, Malepati S, Cheng L, Xiao H, Duong D, Seyfried NT, Wood LB, Rowan MJ, Rangaraju S. Native-state proteomics of Parvalbumin interneurons identifies novel molecular signatures and metabolic vulnerabilities to early Alzheimer's disease pathology. BioRxiv 2023 PMID: 37292756, DOI: 10.1101/2023.05.17.541038.Peer-Reviewed Original ResearchStress primes microglial polarization after global ischemia: Therapeutic potential of progesterone
Espinosa-Garcia C, Sayeed I, Yousuf S, Atif F, Sergeeva E, Neigh G, Stein D. Stress primes microglial polarization after global ischemia: Therapeutic potential of progesterone. Brain Behavior And Immunity 2017, 66: 177-192. PMID: 28648389, DOI: 10.1016/j.bbi.2017.06.012.Peer-Reviewed Original ResearchConceptsAnti-inflammatory cytokinesGlobal ischemiaDays of social defeat stressIncreased pro-inflammatory cytokine expressionPost-ischemiaIntraperitoneal injections of progesteroneNeuroprotective effects of progesteroneMale Sprague-Dawley ratsIschemic strokeAssociated with increased riskMicroglial polarizationSocial defeat stressCA1 hippocampal regionEffects of progesteroneAssociated with increased risk of strokePro-inflammatory cytokine expressionSprague-Dawley ratsNeurotrophic factor expressionInjection of progesteroneAnti-inflammatory mediatorsPro-inflammatory cytokinesExacerbate neuronal lossRisk of strokeM2 phenotype markersPotential therapeutic strategyImpact of Stress on Epilepsy: Focus on Neuroinflammation—A Mini Review
Espinosa-Garcia C, Zeleke H, Rojas A. Impact of Stress on Epilepsy: Focus on Neuroinflammation—A Mini Review. International Journal Of Molecular Sciences 2021, 22: 4061. PMID: 33920037, PMCID: PMC8071059, DOI: 10.3390/ijms22084061.Peer-Reviewed Original ResearchConceptsImpact of stressChronic stressPsychiatric comorbidityPsychiatric comorbiditiesStressor durationDepressive stateAcute stressPoor quality of lifeBrain damageSeizure occurrencePreclinical researchSeizure triggersOutcome severityDisordersEpilepsy outcomeEpilepsy patientsRecurrent seizuresEpilepsyNeurological disordersQuality of lifeSeizuresDeleterious impactDepressionComorbidity
2024
ATR-FTIR spectroscopy to evaluate serum protein expression in a murine cerebral ischemia model
Montes-Narváez O, García-Juárez M, Beltrán-Pérez G, Espinosa-García C, González-Flores O, Delgado-Macuil R. ATR-FTIR spectroscopy to evaluate serum protein expression in a murine cerebral ischemia model. Spectrochimica Acta Part A Molecular And Biomolecular Spectroscopy 2024, 326: 125261. PMID: 39395276, DOI: 10.1016/j.saa.2024.125261.Peer-Reviewed Original ResearchEditorial: Immune system mechanisms impacting the onset of epilepsy and spontaneous seizures
Espinosa-Garcia C, Bahramnejad E, Li Y. Editorial: Immune system mechanisms impacting the onset of epilepsy and spontaneous seizures. Frontiers In Molecular Neuroscience 2024, 17: 1487871. PMID: 39318421, PMCID: PMC11420127, DOI: 10.3389/fnmol.2024.1487871.Peer-Reviewed Original ResearchProximity Labeling Proteomics Reveals Kv1.3 Potassium Channel Immune Interactors in Microglia
Bowen C, Nguyen H, Lin Y, Bagchi P, Natu A, Espinosa-Garcia C, Werner E, Kumari R, Brandelli A, Kumar P, Tobin B, Wood L, Faundez V, Wulff H, Seyfried N, Rangaraju S. Proximity Labeling Proteomics Reveals Kv1.3 Potassium Channel Immune Interactors in Microglia. Molecular & Cellular Proteomics 2024, 23: 100809. PMID: 38936775, DOI: 10.1016/j.mcpro.2024.100809.Peer-Reviewed Original ResearchDisease-associated microgliaPDZ-binding domainImmune signaling proteinsInflammatory responseProinflammatory signaling moleculesInhibition of Kv1.3Resident immune cellsSignaling proteinsKv1.3 potassium channelsC-terminusC-terminal PDZ-binding domainMicroglia transitionTLR4-mediated activationLipopolysaccharide-induced inflammatory responseKv1.3 blockadeImmune cellsPotential interacting proteinsInflammatory statePotassium channelsInflammatory contextKv1.3Flux of potassium ionsTLR4-induced inflammatory responsesFlow cytometryGlycolytic responseNative-state proteomics of Parvalbumin interneurons identifies unique molecular signatures and vulnerabilities to early Alzheimer’s pathology
Kumar P, Goettemoeller A, Espinosa-Garcia C, Tobin B, Tfaily A, Nelson R, Natu A, Dammer E, Santiago J, Malepati S, Cheng L, Xiao H, Duong D, Seyfried N, Wood L, Rowan M, Rangaraju S. Native-state proteomics of Parvalbumin interneurons identifies unique molecular signatures and vulnerabilities to early Alzheimer’s pathology. Nature Communications 2024, 15: 2823. PMID: 38561349, PMCID: PMC10985119, DOI: 10.1038/s41467-024-47028-7.Peer-Reviewed Original ResearchConceptsBiotinylation of proteinsAlzheimer's diseasePre-synaptic defectsDecreased mTOR signalingAD pathogenesisAD riskCytoskeletal disruptionProteomic alterationsTranslational activityMolecular insightsMTOR signalingProteomic findingsProteomicsProteomic signatureMolecular signaturesProteinOver-representedProgressive neuropathologyAlzheimer pathologyMouse modelPV-INsParvalbumin interneuronsMitochondriaAssociated with cognitive declineFast-spiking parvalbumin interneurons
2023
Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease
Bowen C, Lin Y, Zeng H, Nguyen H, Xiao H, Kumar P, Duong D, Espinosa‐Garcia C, Wulff H, Seyfried N, Rangaraju S. Immune regulatory roles for Kv1.3 channels in Microglia in Alzheimer’s Disease. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.078851.Peer-Reviewed Original ResearchKv1.3 blockadeKv1.3 channelsKv1.3 blockersMouse model of AD pathologyBlockade of Kv1.3Immune regulatory roleWestern blottingAlzheimer's diseaseCMV-Cre miceChannels in vitroPDZ-binding motifPlasma membrane proteinsInhibition in vivoCell type-specificPharmacological blockadeOlder 5XFAD miceCMV-CreFunction of microgliaChannel abundanceKv1.3Mouse modelBiotin ligaseFlow cytometryTrafficking proteinsBiotinylated proteinsAge‐Dependent Changes in Perineuronal Nets and Associated Parvalbumin Interneurons in the 5xFAD Amyloidosis Mouse Model
Nelson R, Rangaraju S, Rayaprolu S, Kumar P, Espinosa‐Garcia C, Xiao H. Age‐Dependent Changes in Perineuronal Nets and Associated Parvalbumin Interneurons in the 5xFAD Amyloidosis Mouse Model. Alzheimer's & Dementia 2023, 19 DOI: 10.1002/alz.083101.Peer-Reviewed Original ResearchPV+ neuronsWild typePerineuronal netsAlzheimer's diseaseAD-like pathologyCo-localizationMouse modelAmyloid-betaFast-spiking interneuronsEarly amyloidosisRegulate synaptic transmissionSynaptic maintenanceExtracellular matrix structurePlaque proximityAge-dependent mannerMonths of agePhysiological roleImmunofluorescence microscopyNeuronal roleNeurodegenerative diseasesParvalbumin-positiveParvalbumin interneuronsAge-dependent changesSynaptic transmissionProximal dendritesProfound seizure suppression and disease modification by targeting JAK1, a key driver of a pro-epileptogenic gene network.
Hoffman OR, Patterson A, Gohar E, Coleman E, Schoenike B, Espinosa-Garcia C, Paredes F, Dingledine R, Maguire J, Roopra AS. Profound seizure suppression and disease modification by targeting JAK1, a key driver of a pro-epileptogenic gene network. BioRxiv 2023 PMID: 37662337, DOI: 10.1101/2023.08.07.552299.Peer-Reviewed Original Research