2023
Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification
Shortreed N, Panicker A, Mangalaparthi K, Zhong J, Pandey A, Griffiths L. Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification. Journal Of Proteomics 2023, 281: 104906. PMID: 37059220, PMCID: PMC10399726, DOI: 10.1016/j.jprot.2023.104906.Peer-Reviewed Original ResearchConceptsAdaptive immune responsesImmune responseAntigen identificationPreparation of protein extractsProtein extractsIdentification of proteinsImmunoproteomic approachAffinity chromatography columnIntra-experimental variabilityTrifluoroacetic acidPre-clinicalUnique antigensDisruption methodsTMT labelingLC-MS/MSHypothesis-driven approachIncreased total numberAntigenLC-MS/MS analysisAntigenic proteinsMass spectrometry timeProteinHigh-throughputLongitudinal mannerImmunoprecipitation
2021
Shotgun Immunoproteomics for Identification of Nonhuman Leukocyte Antigens Associated With Cellular Dysfunction in Heart Transplant Rejection
Gates K, Panicker A, Biendarra-Tiegs S, Vetr N, Higuita M, Nelson T, Pereira N, Griffiths L. Shotgun Immunoproteomics for Identification of Nonhuman Leukocyte Antigens Associated With Cellular Dysfunction in Heart Transplant Rejection. Transplantation 2021, 106: 1376-1389. PMID: 34923540, DOI: 10.1097/tp.0000000000004012.Peer-Reviewed Original ResearchMeSH KeywordsGraft RejectionHeart TransplantationHLA AntigensHumansImmunoglobulin GProteomicsTissue DonorsConceptsAntibody-mediated rejectionHeart transplant recipientsAntigen-specific responsesNon-HLAPrevalence antigensAcute rejectionTransplant recipientsAbsence of donor-specific anti-HLA antibodiesDonor-specific anti-HLA antibodiesNon-HLA antibody responsesAntibody-mediated rejection patientsNon-AMR patientsAnti-HLA antibodiesHeart transplant rejectionPatient immunoglobulin GHigh-prevalence antigenEndothelial cell functionEndomyocardial biopsyAllograft rejectionGraft rejectionHeart biopsiesTransplant rejectionAntibody responseImmunofluorescence stainingPatients