Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification
Shortreed N, Panicker A, Mangalaparthi K, Zhong J, Pandey A, Griffiths L. Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification. Journal Of Proteomics 2023, 281: 104906. PMID: 37059220, PMCID: PMC10399726, DOI: 10.1016/j.jprot.2023.104906.Peer-Reviewed Original ResearchConceptsAdaptive immune responsesImmune responseAntigen identificationPreparation of protein extractsProtein extractsIdentification of proteinsImmunoproteomic approachAffinity chromatography columnIntra-experimental variabilityTrifluoroacetic acidPre-clinicalUnique antigensDisruption methodsTMT labelingLC-MS/MSHypothesis-driven approachIncreased total numberAntigenLC-MS/MS analysisAntigenic proteinsMass spectrometry timeProteinHigh-throughputLongitudinal mannerImmunoprecipitation