2023
Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification
Shortreed N, Panicker A, Mangalaparthi K, Zhong J, Pandey A, Griffiths L. Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification. Journal Of Proteomics 2023, 281: 104906. PMID: 37059220, PMCID: PMC10399726, DOI: 10.1016/j.jprot.2023.104906.Peer-Reviewed Original ResearchConceptsAdaptive immune responsesImmune responseAntigen identificationPreparation of protein extractsProtein extractsIdentification of proteinsImmunoproteomic approachAffinity chromatography columnIntra-experimental variabilityTrifluoroacetic acidPre-clinicalUnique antigensDisruption methodsTMT labelingLC-MS/MSHypothesis-driven approachIncreased total numberAntigenLC-MS/MS analysisAntigenic proteinsMass spectrometry timeProteinHigh-throughputLongitudinal mannerImmunoprecipitation
2020
Immunoproteomic identification of in vivo response toward residual antigenic proteins in xenogeneic heart valve biomaterials
Shortreed N, Panicker A, Dalgliesh A, Gates K, Griffiths L. Immunoproteomic identification of in vivo response toward residual antigenic proteins in xenogeneic heart valve biomaterials. The Journal Of Immunology 2020, 204: 161.9-161.9. DOI: 10.4049/jimmunol.204.supp.161.9.Peer-Reviewed Original ResearchCurrent heart valve prosthesesContext of tissue engineeringValve replacementTissue engineeringManagement of valvular heart diseaseECM scaffoldsValvular heart diseaseHeart valve prosthesesBovine pericardiumHumoral IgG responseNew Zealand white rabbitsZealand white rabbitsAntigen burdenNative BPIgG responsesTherapeutic approachesBiomaterialsExtracellular matrixValve prosthesisHeart diseaseRabbit modelRegenerative capacityAntigenTreatment groupsScaffolds