2023
Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification
Shortreed N, Panicker A, Mangalaparthi K, Zhong J, Pandey A, Griffiths L. Optimization of a high-throughput shotgun immunoproteomics pipeline for antigen identification. Journal Of Proteomics 2023, 281: 104906. PMID: 37059220, PMCID: PMC10399726, DOI: 10.1016/j.jprot.2023.104906.Peer-Reviewed Original ResearchConceptsAdaptive immune responsesImmune responseAntigen identificationPreparation of protein extractsProtein extractsIdentification of proteinsImmunoproteomic approachAffinity chromatography columnIntra-experimental variabilityTrifluoroacetic acidPre-clinicalUnique antigensDisruption methodsTMT labelingLC-MS/MSHypothesis-driven approachIncreased total numberAntigenLC-MS/MS analysisAntigenic proteinsMass spectrometry timeProteinHigh-throughputLongitudinal mannerImmunoprecipitation
2020
Role of non-HLA antigens in cardiac transplant rejection
Panicker A, Gates K, Biendarra-Tiegs S, Vetr N, Higuita M, Nelson T, Pereira N, Griffiths L. Role of non-HLA antigens in cardiac transplant rejection. The Journal Of Immunology 2020, 204: 161.7-161.7. DOI: 10.4049/jimmunol.204.supp.161.7.Peer-Reviewed Original ResearchNon-HLA antigensNon-HLA antibodiesHeart transplant patientsHuman leukocyte antigenImmune responseTransplant patientsTransplant rejectionDonor-specific anti-HLA antibodiesTerm survivalNon-HLA antibody responsesSpecific anti-HLA antibodiesAnti-HLA antibodiesCardiac allograft vasculopathyHeart transplant recipientsCardiac transplant rejectionHeart transplant rejectionCell mediated rejectionAdaptive immune responsesLong-term survivalShort-term survivalAllograft vasculopathyRejection incidenceAcute rejectionTransplant recipientsAntigen expression