2001
The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210
Karpen H, Bukowski J, Hughes T, Gratton J, Sessa W, Gailani M. The Sonic Hedgehog Receptor Patched Associates with Caveolin-1 in Cholesterol-rich Microdomains of the Plasma Membrane* 210. Journal Of Biological Chemistry 2001, 276: 19503-19511. PMID: 11278759, DOI: 10.1074/jbc.m010832200.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBinding SitesBlotting, WesternCaveolin 1CaveolinsCell MembraneCholesterolCOS CellsDNA, ComplementaryDrosophila ProteinsElectrophoresis, Polyacrylamide GelGlutathione TransferaseHumansImmunohistochemistryMembrane MicrodomainsMembrane ProteinsMicroscopy, ConfocalModels, BiologicalMolecular Sequence DataMutationPatched ReceptorsPrecipitin TestsProtein BindingProtein Structure, TertiaryProtein TransportReceptors, Cell SurfaceReceptors, G-Protein-CoupledRecombinant Fusion ProteinsSignal TransductionSmoothened ReceptorSubcellular FractionsTime FactorsConceptsCholesterol-rich microdomainsRaft microdomainsCaveolin-1Receptor complexEarly embryonic patterningFractionation studiesHedgehog receptor complexCaveolin-enriched microdomainsBuoyant density fractionsEmbryonic patterningHh proteinsLipid raftsSubcellular localizationPlasma membranePatchedPlasmalemmal cholesterolProtein experimentsImmunoprecipitation studiesSmoothenedMicrodomainsConfocal microscopyImmunocytochemistry dataComplexesMembraneDrosophila
1999
Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters
Segal S, Brett S, Sessa W. Codistribution of NOS and caveolin throughout peripheral vasculature and skeletal muscle of hamsters. American Journal Of Physiology 1999, 277: h1167-h1177. PMID: 10484439, DOI: 10.1152/ajpheart.1999.277.3.h1167.Peer-Reviewed Original ResearchConceptsCaveolin-3Caveolin-1Skeletal muscleCaveolin isoformsCoat proteinCaveolinFunctional interactionIntact organismEnzyme isoformsNitric oxide synthase activitySmooth muscle cellsMuscle fibersOxide synthase activityVascular smooth muscleSynthase activitySkeletal muscle fibersMuscle cellsIsoformsCell systemSystemic vasculatureVena cavaFemoral arteryAbdominal aortaPeripheral vasculatureEndothelial cells
1998
Neuronal nitric oxide synthase is expressed in principal cell of collecting duct
Wang X, Lu M, Gao Y, Papapetropoulos A, Sessa W, Wang W. Neuronal nitric oxide synthase is expressed in principal cell of collecting duct. American Journal Of Physiology 1998, 275: f395-f399. PMID: 9729512, DOI: 10.1152/ajprenal.1998.275.3.f395.Peer-Reviewed Original ResearchConceptsNeuronal nitric oxide synthaseEndothelial nitric oxide synthasePresence of nNOSNitric oxide synthaseOxide synthasePrincipal cellsImmunocytochemical studyPresence of mRNART-PCR techniqueRat neuronal nitric oxide synthaseNegative immunostainingNNOS antibodyRat CCDRat kidneyImmunocytochemical methodsRT-PCRImmunoreactivityRatsDietCellsLight microscopyDuctSynthaseGene-specific primersKidney
1992
High endothelin-1 immunoreactivity in Crohn's disease and ulcerative colitis
Murch SH, Braegger CP, MacDonald T, Sessa W. High endothelin-1 immunoreactivity in Crohn's disease and ulcerative colitis. The Lancet 1992, 339: 381-385. PMID: 1346658, DOI: 10.1016/0140-6736(92)90077-g.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentChildColitis, UlcerativeCrohn DiseaseEndothelinsFemaleHLA-DR AntigensHumansImmunohistochemistryIntestinal MucosaIntestinesMaleConceptsCrohn's disease patientsInflammatory bowel diseaseDisease patientsUlcerative colitisCrohn's diseaseBowel diseaseEndothelin-1Lamina propriaChronic inflammatory bowel diseaseEndothelin-1 immunoreactivitySubmucosal blood vesselsUlcerative colitis patientsTissue samplesUlcerative colitis groupEndothelin concentrationsImmunological hypersensitivityIntestinal ischaemiaColitis groupColitis patientsEndothelin productionInflammatory cellsPotent vasoconstrictorVascular abnormalitiesDisease groupImmunoreactive cells