2021
Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2003
Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD
Thomson RB, Mentone S, Kim R, Earle K, Delpire E, Somlo S, Aronson PS. Histopathological analysis of renal cystic epithelia in the Pkd2WS25/- mouse model of ADPKD. American Journal Of Physiology. Renal Physiology 2003, 285: f870-f880. PMID: 12851251, DOI: 10.1152/ajprenal.00153.2003.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsCadherinsDisease Models, AnimalEpitheliumGene SilencingImmunohistochemistryKidneyMiceMicroscopy, ElectronMicroscopy, Electron, ScanningPolycystic Kidney, Autosomal DominantProtein Kinase D2Protein KinasesSodium-Potassium-Chloride SymportersSodium-Potassium-Exchanging ATPaseSolute Carrier Family 12, Member 2Tissue DistributionConceptsCell polarityKsp-cadherinBasolateral membraneRenal cystic epitheliaEpithelial cell polarityEpithelial cellsSpecific membrane proteinsCell dedifferentiationEpithelial cell dedifferentiationE-cadherinRenal epithelial cellsCystic epithelial cellsMembrane proteinsPhenotypic transitionCl(-) cotransporter NKCC1Expression levelsATPaseSecretory isoformEarly cystsTransepithelial secretionImmunocytochemical analysisUltrastructural analysisAutosomal dominant polycystic kidney diseaseDominant polycystic kidney diseasePolycystic kidney disease
1999
Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice
Igarashi P, Shashikant C, Thomson R, Whyte D, Liu-Chen S, Ruddle F, Aronson P. Ksp-cadherin gene promoter. II. Kidney-specific activity in transgenic mice. American Journal Of Physiology 1999, 277: f599-f610. PMID: 10516285, DOI: 10.1152/ajprenal.1999.277.4.f599.Peer-Reviewed Original ResearchConceptsKsp-cadherinEscherichia coli lacZ reporter geneTissue-specific memberKsp-cadherin gene promoterRepeat-induced geneTranscriptional start siteTissue-specific expressionDifferentiation-specific expressionBeta-galactosidase enzyme activityTransgene integration sitesKidney-specific expressionLacZ reporter geneTransgenic miceEpithelial cellsKidney-specific activityStart siteRegulatory elementsGene promoterReporter genePronuclear microinjectionKb upstreamUreteric budTubular epithelial cellsDevelopmental expressionIntegration sites
1998
cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin
Thomson RB, Ward DC, Quaggin SE, Igarashi P, Muckler ZE, Aronson PS. cDNA Cloning and Chromosomal Localization of the Human and Mouse Isoforms of Ksp-Cadherin. Genomics 1998, 51: 445-451. PMID: 9721215, DOI: 10.1006/geno.1998.5402.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCadherinsCell Adhesion MoleculesChromosome MappingChromosomes, Human, Pair 16Cloning, MolecularHumansIn Situ Hybridization, FluorescenceKidneyMiceMice, Inbred StrainsMolecular Sequence DataMultigene FamilyRNA, MessengerSequence Analysis, DNASequence Homology, Amino AcidConceptsMouse isoformsKsp-cadherinPutative N-glycosylation sitesInferred amino acid sequenceSingle ancestral geneCharacteristic protein domainsCadherin family membersKidney-specific memberN-glycosylation sitesAmino acid sequenceMammalian genomesAncestral geneGene duplicationSyntenic regionsChromosomal localizationComplete cDNAGene mapsProtein domainsSequence motifsGene sequencesCDNA cloningCell adhesion moleculeAcid sequenceNorthern analysisChromosome 8
1995
Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *
Thomson R, Igarashi P, Biemesderfer D, Kim R, Abu-Alfa A, Soleimani M, Aronson P. Isolation and cDNA Cloning of Ksp-cadherin, a Novel Kidney-specific Member of the Cadherin Multigene Family *. Journal Of Biological Chemistry 1995, 270: 17594-17601. PMID: 7615566, DOI: 10.1074/jbc.270.29.17594.Peer-Reviewed Original ResearchConceptsKidney-specific memberMultigene familyKsp-cadherinCDNA cloningCharacteristic cysteine residuesT7 expression systemClassical cadherinsCytoplasmic domainCysteine residuesExpression systemTissue developmentImmunolocalization studiesRecognition sequenceNorthern analysisCadherinDemonstrated roleCellular recognitionMolecular analysisLI-cadherinHPT-1Basolateral membraneCloningRenal tubular epithelial cellsEpithelial cellsProtein