2002
COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle
Siddiqi SA, Gorelick FS, Mahan JT, Mansbach CM. COPII proteins are required for Golgi fusion but not for endoplasmic reticulum budding of the pre-chylomicron transport vesicle. Journal Of Cell Science 2002, 116: 415-427. PMID: 12482926, DOI: 10.1242/jcs.00215.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesApolipoprotein B-48Apolipoproteins BCells, CulturedChylomicronsCOP-Coated VesiclesEndoplasmic ReticulumEpithelial CellsGolgi ApparatusIntestinal AbsorptionIntestinal MucosaLipid MetabolismMaleMembrane FusionMicroscopy, ElectronMonomeric GTP-Binding ProteinsProtein TransportRatsSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsPre-chylomicron transport vesicleCOPII proteinsEndoplasmic reticulumTransport vesiclesBudding of vesiclesSucrose density centrifugationNascent proteinsCargo proteinsProtein vesiclesMembrane proteinsGolgi complexProteinase K treatmentProteinSec24VesiclesGolgiSar1Intestinal GolgiDensity centrifugationReticulumK treatmentTriton XSec13/31Rbet1COPII
1999
Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat
Shugrue C, Kolen E, Peters H, Czernik A, Kaiser C, Matovcik L, Hubbard A, Gorelick F. Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat. Journal Of Cell Science 1999, 112: 4547-4556. PMID: 10574704, PMCID: PMC5567750, DOI: 10.1242/jcs.112.24.4547.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceCarrier ProteinsCell LineCloning, MolecularCOP-Coated VesiclesDNA, ComplementaryFungal ProteinsGTPase-Activating ProteinsHumansMembrane ProteinsMolecular Sequence DataNuclear Pore Complex ProteinsPhosphoproteinsRatsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidVesicular Transport ProteinsConceptsMammalian orthologuesCOPII coatSequential column chromatographyTwo-hybrid analysisIntracellular vesicular traffickingSmall punctate structuresVesicle-associated proteinLiver cDNA libraryCultured cell linesRat liver cDNA libraryVesicular buddingVesicular traffickingNovel proteinSignificant homologyTarget membraneCDNA libraryPunctate structuresSec13pSec31pIntact cellsP137ProteinOrthologuesCell linesColumn chromatography