2024
Circulating tumor-reactive KIR+CD8+ T cells suppress anti-tumor immunity in patients with melanoma
Lu B, Lucca L, Lewis W, Wang J, Nogueira C, Heer S, Rayon-Estrada V, Axisa P, Reeves S, Buitrago-Pocasangre N, Pham G, Kojima M, Wei W, Aizenbud L, Bacchiocchi A, Zhang L, Walewski J, Chiang V, Olino K, Clune J, Halaban R, Kluger Y, Coyle A, Kisielow J, Obermair F, Kluger H, Hafler D. Circulating tumor-reactive KIR+CD8+ T cells suppress anti-tumor immunity in patients with melanoma. Nature Immunology 2024, 1-10. PMID: 39609626, DOI: 10.1038/s41590-024-02023-4.Peer-Reviewed Original ResearchCD8+ T cellsAnti-tumor immunityRegulatory T cellsT cellsSubpopulation of CD8+ T cellsCytotoxic CD8+ T cellsHuman CD8+ T cellsTumor antigen-specific CD8Impaired anti-tumor immunityTumor antigen-specificPoor overall survivalTumor rejectionKIR expressionOverall survivalTumor antigensImmune evasionCellular mediatorsHuman cancersCD8MelanomaTumorTranscriptional programsFunctional heterogeneityImmunityPatients
2017
Resisting fatal attraction: a glioma oncometabolite prevents CD8+ T cell recruitment
Lucca LE, Hafler DA. Resisting fatal attraction: a glioma oncometabolite prevents CD8+ T cell recruitment. Journal Of Clinical Investigation 2017, 127: 1218-1220. PMID: 28319049, PMCID: PMC5373854, DOI: 10.1172/jci93565.Peer-Reviewed Original ResearchConceptsT cellsRecruitment of CD8Antitumor immune responseT cell recruitmentMigration of CD8Cell lung tumorsSyngeneic gliomaChemokines CXCL9Tumor escapeTumor controlImmune infiltrationMetastatic melanomaClinical trialsMalignant gliomasExpression of STAT1Immune surveillanceLung tumorsImmune responseAggressive cancerCell recruitmentCD8Tumor destructionTumorsGliomasCommon mutations
2003
Activated CD8+ T cells in secondary progressive MS secrete lymphotoxin
Buckle GJ, Höllsberg P, Hafler DA. Activated CD8+ T cells in secondary progressive MS secrete lymphotoxin. Neurology 2003, 60: 702-705. PMID: 12601116, DOI: 10.1212/01.wnl.0000048204.89346.30.Peer-Reviewed Original ResearchMeSH KeywordsAdultAntibodiesCD3 ComplexCD8-Positive T-LymphocytesCell DivisionCell SeparationCells, CulturedCytokinesFemaleFlow CytometryGene FrequencyHumansLymphotoxin-alphaMaleMiddle AgedMultiple Sclerosis, Chronic ProgressiveMultiple Sclerosis, Relapsing-RemittingPolymorphism, Single NucleotideReference ValuesConceptsT cellsNormal controlsSecondary progressive MSCytokine secretion profileFunctional activation statesLymphotoxin secretionProgressive MSActivated CD8Cytokine secretionSecretion profileCytokine genesCD8SecretionSignificant differencesPatientsSignificant increaseActivation stateSingle nucleotide polymorphism analysisPolymorphism analysisNucleotide polymorphism analysisCells
2002
Strength of prior stimuli determines the magnitude of secondary responsiveness in CD8+ T cells
Lim DG, Höllsberg P, Hafler DA. Strength of prior stimuli determines the magnitude of secondary responsiveness in CD8+ T cells. Cellular Immunology 2002, 217: 36-46. PMID: 12425999, DOI: 10.1016/s0008-8749(02)00511-7.Peer-Reviewed Original ResearchConceptsT cellsSecondary responsivenessCostimulatory moleculesInduction of CD8Magnitude of CD8T cell responsesT cell anergyCell anergyCD8Prior stimulusSecondary stimulationPrimary stimulationCell responsesCellular mechanismsFollowing activationPeptide ligandsActivation thresholdStimulationCellsResponsivenessHigh levelsCD4AnergyStimuliStrength of signal
1999
Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy.
Bieganowska K, Höllsberg P, Buckle G, Lim D, Greten T, Schneck J, Altman J, Jacobson S, Ledis S, Hanchard B, Chin J, Morgan O, Roth P, Hafler D. Direct analysis of viral-specific CD8+ T cells with soluble HLA-A2/Tax11-19 tetramer complexes in patients with human T cell lymphotropic virus-associated myelopathy. The Journal Of Immunology 1999, 162: 1765-71. PMID: 9973440, DOI: 10.4049/jimmunol.162.3.1765.Peer-Reviewed Original ResearchMeSH KeywordsAdultB7-1 AntigenCD28 AntigensCD8-Positive T-LymphocytesFemaleGene Products, taxHLA-A2 AntigenHuman T-lymphotropic virus 1HumansLymphocyte ActivationMaleMiddle AgedParaparesis, Tropical SpasticPeptide FragmentsPhenotypeProtein ConformationReceptors, Antigen, T-Cell, alpha-betaReceptors, ChemokineReceptors, Interleukin-2SolubilityConceptsT cellsIL-2RMHC class I tetramersHuman T-cell lymphotropic virusViral-specific CD8Class I tetramersProgressive neurologic diseaseDifferent chemokine receptorsHLA-A2 alleleCentral nervous systemTCR Vbeta chainsReactive CD8TCR usageInflammatory infiltrateVbeta chainsChemokine receptorsNeurologic diseaseImmune responseCD8Lymphotropic virusMyelopathyNervous systemPatientsHTLVClonal expansion
1995
Inhibition of T cell responses by activated human CD8+ T cells is mediated by interferon-gamma and is defective in chronic progressive multiple sclerosis.
Balashov KE, Khoury SJ, Hafler DA, Weiner HL. Inhibition of T cell responses by activated human CD8+ T cells is mediated by interferon-gamma and is defective in chronic progressive multiple sclerosis. Journal Of Clinical Investigation 1995, 95: 2711-2719. PMID: 7769110, PMCID: PMC295954, DOI: 10.1172/jci117973.Peer-Reviewed Original ResearchConceptsAutologous mixed lymphocyte reactionProgressive multiple sclerosisChronic progressive multiple sclerosisInduction of CD8Tact cellsMultiple sclerosisT cellsHuman CD8Suppressive propertiesAutologous T cellsRelapsing-remitting MST cell responsesHuman autoimmune diseasesMixed lymphocyte reactionGM-CSF antibodySuppressive CD8Autologous antigenLymphocyte reactionAutoimmune diseasesBeta antibodyGamma antibodyCD8Proliferative responseSpecific cytokinesNormal controls
1993
MHC-Restricted Depletion of Human Myelin Basic Protein-Reactive T Cells by T Cell Vaccination
Zhang J, Medaer R, Stinissen P, Hafler D, Raus J. MHC-Restricted Depletion of Human Myelin Basic Protein-Reactive T Cells by T Cell Vaccination. Science 1993, 261: 1451-1454. PMID: 7690157, DOI: 10.1126/science.7690157.Peer-Reviewed Original ResearchConceptsAutoreactive T cellsT cell vaccinationT cellsT cell linesCell vaccinationMyelin basic protein-reactive T cellsRegulatory T cell linesMBP-reactive T cellsExperimental autoimmune diseasesReactive T cellsT cell responsesMajor histocompatibility antigensCell linesMyelin basic proteinMultiple sclerosisAutoimmune diseasesHistocompatibility antigensCell responsesVaccinationBasic proteinRecipientsPresent studyCellsCD8Sclerosis
1990
Frequency analysis of CD4+CD8+ T cells cloned with IL-4
Brod S, Purvee M, Benjamin D, Hafler D. Frequency analysis of CD4+CD8+ T cells cloned with IL-4. Cellular Immunology 1990, 125: 426-436. PMID: 2105169, DOI: 10.1016/0008-8749(90)90096-a.Peer-Reviewed Original ResearchConceptsT cell clonesHigh-density CD4Expression of CD8RIL-4IL-2CD8 expressionT cellsIL-4CD8 moleculesCD4 expressionCell clonesPeripheral blood T cellsHigh density CD8Low CD8 expressionSurface CD8 expressionLectin-dependent cytotoxicitySurface of CD4Blood T cellsPattern of CD4Class I MHCNK activityPeripheral bloodCell surface expressionInflammatory responseCD8
1988
The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions.
Sobel RA, Hafler DA, Castro EE, Morimoto C, Weiner HL. The 2H4 (CD45R) antigen is selectively decreased in multiple sclerosis lesions. The Journal Of Immunology 1988, 140: 2210-4. PMID: 2965183, DOI: 10.4049/jimmunol.140.7.2210.Peer-Reviewed Original ResearchConceptsMultiple sclerosisViral encephalitisImmune responseCentral nervous system immune responseSuppressor-inducer functionCentral nervous system tissuePlaque edgeAdjacent white matterIL-2R mAbNervous system tissueMultiple sclerosis lesionsAnti-2H4Anti-CD4Inflammatory cellsMS plaquesImmunohistochemical stainingWhite matterSclerosis lesionsEncephalitisPatientsCellsRare cellsCD8CD4SclerosisRegulation of T cell clone function via CD4 and CD8 molecules. Anti-CD4 can mediate two distinct inhibitory activities.
Blue ML, Hafler DA, Daley JF, Levine H, Craig KA, Breitmeyer JB, Schlossman SF. Regulation of T cell clone function via CD4 and CD8 molecules. Anti-CD4 can mediate two distinct inhibitory activities. The Journal Of Immunology 1988, 140: 376-83. PMID: 2891768, DOI: 10.4049/jimmunol.140.2.376.Peer-Reviewed Original ResearchConceptsReceptor functionAnti-CD4Anti-CD8 monoclonal antibodiesCD3-TiCell receptor functionMajor histocompatibility complex moleculesIntracellular free Ca2CD3 T-cell receptorT cell activationHistocompatibility complex moleculesCytotoxic functionT cell receptor functionCD8 moleculesCD4CD8Distinct inhibitory activityCD4 moleculeCell activationAntigen receptor functionCell receptorMonoclonal antibodiesFree Ca2Dependent proliferationFunctional effectsSoluble antibodies
1987
Functional gamma chain-associated T cell receptors on cerebrospinal fluid-derived natural killer-like T cell clones.
Ang SL, Seidman JG, Peterman GM, Duby AD, Benjamin D, Lee SJ, Hafler DA. Functional gamma chain-associated T cell receptors on cerebrospinal fluid-derived natural killer-like T cell clones. Journal Of Experimental Medicine 1987, 165: 1453-1458. PMID: 3106557, PMCID: PMC2188318, DOI: 10.1084/jem.165.5.1453.Peer-Reviewed Original ResearchConceptsAnti-CD3 mAbT cell clonesCerebrospinal fluidCell clonesIndependent T cell clonesNK-like activityHuman immune responseT cell receptorWT-31IL-2T cellsImmune responseIntracellular calciumCell receptorMAbsTcR gamma proteinGamma proteinSepharose beadsCell surfacePhenotypic analysisCD8CD4PanencephalitisSubacutePatients