Featured Publications
A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis
Newton HJ, Kohler LJ, McDonough JA, Temoche-Diaz M, Crabill E, Hartland EL, Roy CR. A Screen of Coxiella burnetii Mutants Reveals Important Roles for Dot/Icm Effectors and Host Autophagy in Vacuole Biogenesis. PLOS Pathogens 2014, 10: e1004286. PMID: 25080348, PMCID: PMC4117601, DOI: 10.1371/journal.ppat.1004286.Peer-Reviewed Original ResearchConceptsHost autophagyTransposon insertionDot/Icm effectorsDot/Icm systemAutophagosome protein LC3Intracellular growth defectPathogen-occupied vacuolesTransposon insertion mutantsVacuole biogenesisEffector proteinsInsertion mutantsModification enzymesGrowth defectArrayed libraryCentral metabolismIcm systemMutantsMolecular mechanismsVisual screenProtein LC3Host cellsIntracellular replicationGenesIntracellular pathogensRegulatory system
1997
Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages
Roy C, Isberg R. Topology of Legionella pneumophila DotA: an inner membrane protein required for replication in macrophages. Infection And Immunity 1997, 65: 571-578. PMID: 9009315, PMCID: PMC176098, DOI: 10.1128/iai.65.2.571-578.1997.Peer-Reviewed Original ResearchConceptsDotA mutantsAmino acidsMembrane proteinsIntegral cytoplasmic membrane proteinLarge periplasmic domainInner membrane proteinCarboxyl-terminal cytoplasmic domainIntracellular growthCytoplasmic membrane proteinAlkaline phosphatase fusionsL. pneumophila pathogenesisIntracellular growth defectMembrane-spanning domainsStructure-function analysisProtein fractionation studiesPeriplasmic domainDotA proteinTranslational fusionGrowth defectCytoplasmic domainInner membraneHybrid proteinCytoplasmic regionDotA geneC-terminus