2021
A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa
Lin CK, Lee DSW, McKeithen-Mead S, Emonet T, Kazmierczak B. A Primed Subpopulation of Bacteria Enables Rapid Expression of the Type 3 Secretion System in Pseudomonas aeruginosa. MBio 2021, 12: 10.1128/mbio.00831-21. PMID: 34154400, PMCID: PMC8262847, DOI: 10.1128/mbio.00831-21.Peer-Reviewed Original ResearchConceptsType 3 secretion systemSecretion systemT3SS expressionVirulence traitsSpecific virulence traitsHuman disease severityComplex nanomachinesT3SS genesP. aeruginosa cellsReproductive fitnessIsogenic cellsHeterogeneous expressionCell envelopeT3SS effectorsMotility organellesReservoir of cellsCritical virulence traitsGene expressionRegulatory mechanismsSubpopulation of cellsGram-negative pathogensFluorescent reportersDivision timeP. aeruginosaPseudomonas aeruginosa
2020
Hfq and sRNA 179 Inhibit Expression of the Pseudomonas aeruginosa cAMP-Vfr and Type III Secretion Regulons
Janssen KH, Corley JM, Djapgne L, Cribbs JT, Voelker D, Slusher Z, Nordell R, Regulski EE, Kazmierczak BI, McMackin EW, Yahr TL. Hfq and sRNA 179 Inhibit Expression of the Pseudomonas aeruginosa cAMP-Vfr and Type III Secretion Regulons. MBio 2020, 11: 10.1128/mbio.00363-20. PMID: 32546612, PMCID: PMC7298702, DOI: 10.1128/mbio.00363-20.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCyclic AMP Receptor ProteinCyclic AMP-Dependent Protein KinasesGene Expression Regulation, BacterialGene LibraryHost Factor 1 ProteinPromoter Regions, GeneticPseudomonas aeruginosaRegulonRNA, BacterialRNA, Small UntranslatedTranscription, GeneticType III Secretion SystemsVirulence FactorsConceptsSmall noncoding RNAsType III secretion systemSecretion systemNoncoding RNAsGene expressionTranscription factorsExpression libraryGac/Rsm systemType VI secretion systemT3SS gene expressionCAMP-responsive transcription factorRNA-binding proteinVirulence factorsGram-negative opportunistic pathogenRNA chaperonesGlobal regulatorT3SS regulonMutant lackingTarget mRNAsCritical virulence factorImportant virulence factorMRNA targetsHfqRegulonRsm system