2025
Systematic review and meta-analysis of the genetics of peripheral arterial disease
Chaar C, Kim T, Alameddine D, DeWan A, Guzman R, Dardik A, Nardini H, Wallach J, Kullo I, Murray M. Systematic review and meta-analysis of the genetics of peripheral arterial disease. Journal Of Vascular Surgery 2025, 81: a13-a14. DOI: 10.1016/j.jvs.2024.10.063.Peer-Reviewed Original Research
2024
Renal Angptl4 is a key fibrogenic molecule in progressive diabetic kidney disease
Srivastava S, Zhou H, Shenoi R, Morris M, Lainez-Mas B, Goedeke L, Rajendran B, Setia O, Aryal B, Kanasaki K, Koya D, Inoki K, Dardik A, Bell T, Fernández-Hernando C, Shulman G, Goodwin J. Renal Angptl4 is a key fibrogenic molecule in progressive diabetic kidney disease. Science Advances 2024, 10: eadn6068. PMID: 39630889, PMCID: PMC11616692, DOI: 10.1126/sciadv.adn6068.Peer-Reviewed Original ResearchConceptsAngiopoietin-like 4Diabetic kidney diseaseIntegrin B1Fibrogenic moleculesMutant miceSTING pathway activationIncreased fatty acid oxidationProgressive diabetic kidney diseaseDiabetic kidneyKidney diseaseReduced epithelial-to-mesenchymal transitionEpithelial-to-mesenchymal transitionFatty acid oxidationExpression of pro-inflammatory cytokinesTargeted pharmacological therapiesGene expressionMitochondrial damageEndothelial-to-mesenchymal transitionPro-inflammatory cytokinesPathway activationPharmacological therapyControl miceIntegrinAcid oxidationFibrogenic phenotypeBioengineering on the Front Lines
Tonnessen B, Dardik A. Bioengineering on the Front Lines. JAMA Surgery 2024, 160 PMID: 39565609, DOI: 10.1001/jamasurg.2024.5137.Peer-Reviewed Original ResearchPatient-specific, multiscale modelling of neointimal hyperplasia in lower-limb vein grafts using readily available clinical data
Ninno F, Chiastra C, Donadoni F, Dardik A, Strosberg D, Aboian E, Tsui J, Balabani S, Díaz-Zuccarini V. Patient-specific, multiscale modelling of neointimal hyperplasia in lower-limb vein grafts using readily available clinical data. Journal Of Biomechanics 2024, 177: 112428. PMID: 39561605, DOI: 10.1016/j.jbiomech.2024.112428.Peer-Reviewed Original ResearchTime-averaged wall shear stressInflow boundary conditionsBoundary conditionsWall shear stressShear stressMultiscale modelWaveform shapePeripheral arterial diseaseNeointimal hyperplasiaVein graftsClinical dataMechanobiological mechanismsGraft responseMultiscale in silico modelSystolic acceleration timeCarotid artery stenting versus carotid endarterectomy for symptomatic or asymptomatic extracranial carotid stenosis: A national cohort study
Krittanawong C, Ang S, Tangsrivimol J, Qadeer Y, Wang Z, Virk H, Khalid U, Sharma S, Dardik A. Carotid artery stenting versus carotid endarterectomy for symptomatic or asymptomatic extracranial carotid stenosis: A national cohort study. Journal Of Stroke And Cerebrovascular Diseases 2024, 33: 108094. PMID: 39424210, DOI: 10.1016/j.jstrokecerebrovasdis.2024.108094.Peer-Reviewed Original ResearchAsymptomatic carotid stenosisCarotid artery stenosisCarotid stenosisArtery stenosisAdverse eventsCohort studyAsymptomatic extracranial carotid stenosisNational population-based cohort studyPopulation-based cohort studyAssociated with lower adverse eventsAssociated with high mortalityAlternative to CEAExtracranial carotid stenosisSymptomatic carotid stenosisMortality of patientsCarotid artery stentingLower adverse eventsNational cohort studyAsymptomatic patientsClinical characteristicsArtery stentingPatient populationCarotid endarterectomyStenosisPatientsAn International, Expert-Based Delphi Consensus Document on Controversial Issues about TransCarotid Artery Revascularization (TCAR)
Paraskevas K, AbuRahma A, Abularrage C, Clair D, Eldrup-Jorgensen J, Kashyap V, Dardik A, de Borst G, Dermody M, Faggioli G, Hicks C, Kwolek C, Lyden S, Mansilha A, Van Herzeele I, Myrcha P, Leal Lorenzo J, Jim J, Pini R, Secemsky E, Spinelli F, Capoccia L, Stone D, Stoner M, Zeebregts C, Lal B, Schneider P, Malas M, Schermerhorn M. An International, Expert-Based Delphi Consensus Document on Controversial Issues about TransCarotid Artery Revascularization (TCAR). Annals Of Vascular Surgery 2024, 110: 42-53. PMID: 39413993, DOI: 10.1016/j.avsg.2024.09.048.Peer-Reviewed Original ResearchTransfemoral carotid artery stentingTranscarotid artery revascularizationRandomized controlled trialsCarotid endarterectomyConsensus documentArtery revascularizationAlternative therapeutic modalityManagement of patientsCarotid artery stentingCarotid artery stenosisTreatment of restenosisCarotid stenosis patientsSymptomatic patientsStenosis patientsTherapeutic modalitiesCerebrovascular eventsArtery stenosisArtery stentingControlled trialsDelphi consensus processPatientsContraindicationsRevascularizationTranscarotidTrialsAssociation between Preoperative Neutrophil-lymphocyte Ratio and All-cause Mortality in Patients Treated with Thoracic Endovascular Aortic Repair for Degenerative Thoracic Aortic Aneurysm
Kano M, Nishibe T, Dardik A, Iwahashi T, Nakano Y, Shimahara Y. Association between Preoperative Neutrophil-lymphocyte Ratio and All-cause Mortality in Patients Treated with Thoracic Endovascular Aortic Repair for Degenerative Thoracic Aortic Aneurysm. Annals Of Vascular Surgery 2024, 110: 255-264. PMID: 39341560, DOI: 10.1016/j.avsg.2024.07.110.Peer-Reviewed Original ResearchThoracic endovascular aortic repairPatients treated with thoracic endovascular aortic repairDegenerative thoracic aortic aneurysmsPreoperative neutrophil-lymphocyte ratioNeutrophil-lymphocyte ratioThoracic aortic aneurysmAll-cause mortalityEndovascular aortic repairAortic repairAortic aneurysmCut-off values of neutrophil-lymphocyte ratioOptimal cut-off values of neutrophil-lymphocyte ratioElevated preoperative neutrophil-lymphocyte ratioOverall mortalityValue of neutrophil-lymphocyte ratioOptimal cut-off valueAbsolute lymphocyte countAbsolute neutrophil countRisk of overall mortalityLikelihood of all-cause mortalityReceiver operating characteristic curveIschemic heart diseaseLymphocyte countStratify patientsNeutrophil countEndothelial TGF-β Signaling Regulates Endothelial-Mesenchymal Transition During Arteriovenous Fistula Remodeling in Mice With Chronic Kidney Disease
Zhang W, Gonzalez L, Li X, Bai H, Li Z, Taniguchi R, Langford J, Ohashi Y, Thaxton C, Aoyagi Y, Yatsula B, Martin K, Goodwin J, Tellides G, Long X, Shu C, Dardik A. Endothelial TGF-β Signaling Regulates Endothelial-Mesenchymal Transition During Arteriovenous Fistula Remodeling in Mice With Chronic Kidney Disease. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: 2509-2526. PMID: 39297205, PMCID: PMC11593991, DOI: 10.1161/atvbaha.124.320933.Peer-Reviewed Original ResearchChronic kidney diseaseTGF-b signalingArteriovenous fistula patencyArteriovenous fistulaKidney diseaseAVF patencyEndothelial cellsAssociated with endothelial injuryArteriovenous fistula diameterImprove AVF patencyIncreased outward remodelingReduced patencyInduced endothelial-to-mesenchymal transitionAortocaval fistula modelSmooth muscle cell proliferationArteriovenous fistula failureAttenuated EndMTEnd-stage kidney diseaseHuman arteriovenous fistulaeSmooth muscle cellsMuscle cell proliferationEndothelial-to-mesenchymal transitionMouse endothelial cellsInhibition of EndMTEndothelial-mesenchymal transitionEarly thrombus formation is required for eccentric and heterogeneous neointimal hyperplasia under disturbed flow
Bai H, Li Z, Zhang W, Thaxton C, Ohashi Y, Gonzalez L, Kano M, Yatsula B, Hwa J, Dardik A. Early thrombus formation is required for eccentric and heterogeneous neointimal hyperplasia under disturbed flow. Journal Of Thrombosis And Haemostasis 2024, 22: 3614-3628. PMID: 39173878, PMCID: PMC11608155, DOI: 10.1016/j.jtha.2024.07.028.Peer-Reviewed Original ResearchEndothelial cell lossArteriovenous fistulaSmooth muscle cellsNeointimal hyperplasiaHeterogeneous neointimaCell lossThrombus formationMT/mG reporter miceHuman arteriovenous fistulaeInhibit neointimal hyperplasiaEarly thrombus formationAntiplatelet therapyC57BL/6 miceReporter miceAVF failureWistar ratsVenous systemJuxta-anastomotic areaMuscle cellsAnimal modelsVenous outflowEarly thrombusEndothelial cellsArterial inflowThrombusWound Healing Society 2023 update on guidelines for arterial ulcers
Federman D, Dardik A, Shapshak D, Ueno C, Masterson L, Hopf H, Abdullah N, Junkins S, Mostow E. Wound Healing Society 2023 update on guidelines for arterial ulcers. Wound Repair And Regeneration 2024, 32: 619-629. PMID: 39021056, DOI: 10.1111/wrr.13204.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsRelevant new findingsLevel of evidenceEmergency medicine physiciansWound bed preparationAdjuvant therapySociety guidelinesWound Healing SocietyMedicine physiciansVascular surgeonsPlastic surgeonsInfection controlLong-term maintenanceBed preparationArterial ulcersUlcer managementWound healingArterial insufficiency ulcersSurgeonsWoundAdvisory PanelUlcersGuidelinesSurgeryHealing SocietyTherapyHeterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling
Ohashi Y, Protack C, Aoyagi Y, Gonzalez L, Thaxton C, Zhang W, Kano M, Bai H, Yatsula B, Alves R, Hoshina K, Schneider E, Long X, Perry R, Dardik A. Heterogeneous gene expression during early arteriovenous fistula remodeling suggests that downregulation of metabolism predicts adaptive venous remodeling. Scientific Reports 2024, 14: 13287. PMID: 38858395, PMCID: PMC11164895, DOI: 10.1038/s41598-024-64075-8.Peer-Reviewed Original ResearchConceptsGene expression patternsArteriovenous fistula groupArteriovenous fistulaExpression patternsHeterogeneous gene expressionGene expression changesOutcomes of arteriovenous fistulaVenous remodelingArteriovenous fistula maturationPostoperative day 7Reduce patient morbidityRNA sequencingUpregulation of metabolismBioinformatics analysisGene expressionDownregulation of metabolismMetabolic pathwaysExpression changesGenesAVF maturationAVF remodelingFistula creationC57BL/6 miceClinical outcomesPatient morbidityA surgeon-scientist's approach to improving arteriovenous fistula patency
Dardik A. A surgeon-scientist's approach to improving arteriovenous fistula patency. JVS Vascular Science 2024, 5: 100207. PMID: 38975292, PMCID: PMC11225657, DOI: 10.1016/j.jvssci.2024.100207.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsA new rat model of aortic sympathetic denervation
Pavlyha M, Dardik A. A new rat model of aortic sympathetic denervation. JVS Vascular Science 2024, 5: 100205. PMID: 38962560, PMCID: PMC11219950, DOI: 10.1016/j.jvssci.2024.100205.Commentaries, Editorials and LettersModelling lower-limb peripheral arterial disease using clinically available datasets: impact of inflow boundary conditions on hemodynamic indices for restenosis prediction
Ninno F, Chiastra C, Colombo M, Dardik A, Strosberg D, Aboian E, Tsui J, Bartlett M, Balabani S, Díaz-Zuccarini V. Modelling lower-limb peripheral arterial disease using clinically available datasets: impact of inflow boundary conditions on hemodynamic indices for restenosis prediction. Computer Methods And Programs In Biomedicine 2024, 251: 108214. PMID: 38759252, DOI: 10.1016/j.cmpb.2024.108214.Peer-Reviewed Original ResearchComputational fluid dynamics resultsComputational fluid dynamicsInlet boundary conditionsInlet BCBoundary conditionsLow time-averaged WSSComputational fluid dynamics simulationsTime-averaged WSSNear-wall hemodynamicsInflow boundary conditionsInlet waveformCFD simulationsInflow conditionsFluid dynamicsInletSurface areaWaveformFlow velocityGeometry reconstructionSimulationAccurate estimationVelocity waveformsDoppler ultrasound imagingAcquisition timeScanningClinician perspectives regarding CYP2C19 genotype testing in patients with critical limb ischemia: A Delphi approach.
Regan C, Scierka L, Dardik A, Tonnessen B, Iyad Ochoa Chaar C, Ionescu C, Aboian E, Cardella J, Nassiri N, Guzman R, Attaran R, Nagpal S, Shah S, Smolderen K, Mena-Hurtado C. Clinician perspectives regarding CYP2C19 genotype testing in patients with critical limb ischemia: A Delphi approach. Vascular 2024, 17085381241246318. PMID: 38669051, DOI: 10.1177/17085381241246318.Peer-Reviewed Original ResearchDual antiplatelet therapyPeripheral arterial diseaseCYP2C19 testingCYP2C19 mutationsHeterogeneity of treatment effectsPeripheral vascular interventionsConsensus statementKnowledge questionsAntiplatelet therapyGenetic testingVascular surgeryAcademic health systemBarriers to implementationInterquartile rangeCYP2C19 genotype testingVascular diseaseInterventional cardiologyModified Delphi methodPatient's metabolic statusManagement of patientsCritical limb ischemiaPerceived barriersPeripheral vascular diseaseArterial vascular diseaseClinician perspectivesDisturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia
Bai H, Varsanik M, Thaxton C, Ohashi Y, Gonzalez L, Zhang W, Aoyagi Y, Kano M, Yatsula B, Li Z, Pocivavsek L, Dardik A. Disturbed flow in the juxta-anastomotic area of an arteriovenous fistula correlates with endothelial loss, acute thrombus formation, and neointimal hyperplasia. AJP Heart And Circulatory Physiology 2024, 326: h1446-h1461. PMID: 38578237, PMCID: PMC11380968, DOI: 10.1152/ajpheart.00054.2024.Peer-Reviewed Original ResearchConceptsEndothelial cell lossOutflow veinChronic kidney diseaseJuxta-anastomotic areaMouse AVF modelNeointimal hyperplasiaThrombus formationAcute thrombus formationCell lossHuman AVF maturationGene OntologyAVF maturationAVF patencyEarly thrombus formationArteriovenous fistulaC57BL/6 miceClinical failureWistar ratsEndothelial lossKidney diseaseImmune responseEndothelial cellsHyperplasiaMiceAVFLeveraging Artificial Intelligence to Optimize the Care of Peripheral Artery Disease Patients
Song J, Tomihama R, Roh D, Cabrera A, Dardik A, Kiang S. Leveraging Artificial Intelligence to Optimize the Care of Peripheral Artery Disease Patients. Annals Of Vascular Surgery 2024, 107: 48-54. PMID: 38582202, DOI: 10.1016/j.avsg.2023.11.057.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsReduced Muscle Mass and Muscle Quality in Patients with Intermittent Claudication due to Peripheral Artery Disease
Nishibe T, Dardik A, Akiyama S, Kano M, Fukuda S, Koizumi J, Nishibe M. Reduced Muscle Mass and Muscle Quality in Patients with Intermittent Claudication due to Peripheral Artery Disease. Annals Of Vascular Surgery 2024, 105: 275-281. PMID: 38570014, DOI: 10.1016/j.avsg.2023.12.094.Peer-Reviewed Original ResearchSkeletal muscle massPeripheral arterial diseaseBioelectrical impedance analysisAnkle-brachial indexAnkle-brachial pressure indexLeg skeletal muscle massLeg ischemiaMuscle massIntermittent claudicationLeg muscle massMuscle qualityArtery diseaseAssociated with reduced muscle massTokyo Medical University HospitalAssociated with skeletal muscle myopathyReduced muscle massMedical University HospitalLeg muscle qualitySkeletal muscle myopathyUniversity HospitalMuscle myopathyPatientsIschemiaClaudicationLower extremitiesWhy selective screening for asymptomatic carotid stenosis is currently appropriate: a special report
Paraskevas K, Dardik A, Schermerhorn M, Liapis C, Mansilha A, Lal B, Gray W, Brown M, Myrcha P, Lavie C, Zeebregts C, Secemsky E, Saba L, Blecha M, Gurevich V, Silvestrini M, Blinc A, Svetlikov A, Fernandes e Fernandes J, Schneider P, Gloviczki P, White C, AbuRahma A. Why selective screening for asymptomatic carotid stenosis is currently appropriate: a special report. Expert Review Of Cardiovascular Therapy 2024, 22: 159-165. PMID: 38480465, DOI: 10.1080/14779072.2024.2330660.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsScreening programMass screening programPopulation-wide screening programsRisk factor modificationHigh riskHigh risk of strokeMyocardial infarctionInitiation of preventive measuresRisk of strokeIntensive medical therapyAsymptomatic carotid artery stenosisFactor modificationCost-effectiveLifestyle measuresPrevent future strokeAsymptomatic carotid stenosisTargeted screeningCarotid artery stenosisFuture strokeScreening of populationsCarotid revascularization proceduresProphylactic carotid interventionMedical therapyPreventive measuresCV eventsA new large animal model in venous thromboembolism
Pavlyha M, Dardik A. A new large animal model in venous thromboembolism. JVS Vascular Science 2024, 5: 100201. PMID: 38681682, PMCID: PMC11047774, DOI: 10.1016/j.jvssci.2024.100201.Commentaries, Editorials and Letters