Nanoparticle-Binding Immunoglobulins Predict Variable Complement Responses in Healthy and Diseased Cohorts
Li Y, Saba L, Scheinman R, Banda N, Holers M, Monte A, Dylla L, Moghimi S, Simberg D. Nanoparticle-Binding Immunoglobulins Predict Variable Complement Responses in Healthy and Diseased Cohorts. ACS Nano 2024, 18: 28649-28658. PMID: 39395006, PMCID: PMC11651220, DOI: 10.1021/acsnano.4c05087.Peer-Reviewed Original ResearchConceptsPegylated liposomal doxorubicinComplement activationPredictive valuePlasma concentrationsC-reactive protein levelsAnti-PEG IgGTriggers proinflammatory responsesAnti-PEG IgMEfficacy of nanomedicinesPoor predictive valueAnti-PEG antibodiesLiposomal doxorubicinIron oxide nanowormsHealthy donorsHigher complement activationAcute infectionInflammatory disease conditionsChronic inflammationProinflammatory responseSystemic administrationComplement cascadeComplement factorsComplement responseIgMProtein levelsValidation of dot blot immunoassay for measurement of complement opsonization of nanoparticles
Li Y, Monte A, Dylla L, Moghimi S, Simberg D. Validation of dot blot immunoassay for measurement of complement opsonization of nanoparticles. Journal Of Immunological Methods 2024, 528: 113668. PMID: 38574804, PMCID: PMC11023749, DOI: 10.1016/j.jim.2024.113668.Peer-Reviewed Original ResearchMeSH KeywordsComplement ActivationComplement C3aComplement C5Complement C5aComplement Membrane Attack ComplexImmunoassayNanoparticlesOpsonizationConceptsDeposition of C3Complement activation markersSurface C3 depositionMembrane attack complex C5b-9Activation markersDot blot immunoassayC3 depositionAnaphylatoxins C3aImmune responseNanomedicine researchC5b-9Complement attackComplement activationBlot immunoassayFluid phase markerMarkersPhase markersImmunoassaySurface resultsPathway specificityAssembly of C3 convertaseComplementNanoparticles