2025
Structural basis for the interaction between the Drosophila RTK Sevenless (dROS1) and the GPCR BOSS
Zhang J, Tsutsui Y, Li H, Li T, Wang Y, Laraki S, Alarcon-Frias S, Stayrook S, Klein D. Structural basis for the interaction between the Drosophila RTK Sevenless (dROS1) and the GPCR BOSS. Nature Communications 2025, 16: 808. PMID: 39827240, PMCID: PMC11743138, DOI: 10.1038/s41467-025-55943-6.Peer-Reviewed Original ResearchConceptsFibronectin type IIIExtracellular regionReceptor tyrosine kinasesR7 photoreceptor cellsN-terminal domainCryo-EM structureC-terminal peptideDownstream signaling pathwaysDrosophila homologueBeta-strandsHelical hairpinHuman orthologHydrogen-deuterium exchange mass spectrometryMutagenesis studiesStructural basisRegulatory functionsSignaling pathwayTyrosine kinaseLigand bindingSevenlessComplex predictionBinding epitopeHDX-MSPhotoreceptor cellsBinding interactions
1998
Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains*
Kavran J, Klein D, Lee A, Falasca M, Isakoff S, Skolnik E, Lemmon M. Specificity and Promiscuity in Phosphoinositide Binding by Pleckstrin Homology Domains*. Journal Of Biological Chemistry 1998, 273: 30497-30508. PMID: 9804818, DOI: 10.1074/jbc.273.46.30497.Peer-Reviewed Original ResearchConceptsPleckstrin homology domainPH domainGrp1 PH domainD-myo-inositolParticular phosphoinositidesPhosphoinositide bindingHomology domainDependent membrane recruitmentDifferent PH domainsPH domain bindsSmall protein modulesSoluble inositol phosphatesMembrane recruitmentDomain bindsProtein modulesSpecific phosphoinositideMammalian cellsPlasma membraneSingle speciesAbundant speciesMultiple phosphoinositidesCellular membranesPhosphoinositidePI 3Clear specificity