Parse Biosciences
Overview
The Cell is the Reaction Vessel
The Evercode™ solution from Parse Biosciences converts each cell or nucleus into an individualized reaction compartment. This approach steps away from the limitations of hardware-based offerings by evolving the assay for a more elegant, simple solution to single cell (scRNA-Seq) and single nuclei sequencing (snRNA-Seq).
Fix Now, Run Later
Fix cells or nuclei as they're available to lock in the biology. After a 30 minute fixation, samples are stable for 6 months. Whether working on a timecourse study, sharing samples between laboratories, or segregating sample preparation from core lab space, fixation provides the flexibility to work the way you desire.
No Instrument Required
Conventional droplet-based single-cell technologies struggle as cell or experiment sizes change. Parse makes it easy to scale your experiments regardless of cell size or sample type. The Evercode™ split-pool combinatorial barcoding technology, originally based on the approach published in Science and known widely as SPLiT-Seq, is accessible to any standard biology lab.
Sample Submission
Project Consultation - We strongly recommend a consultation meeting before starting your first Single Cell project. Consultations are free for investigators utilizing the KMSR's single cell service. To request a meeting please contact Guilin Wang (guilin.wang@yale.edu).
Scheduling Your Experiment - The single cell service is by appointment only. You must have an appointment prior to submitting samples. Submission appointments should be made a minimum of one week in advance of your anticipated submission date. This prevents scheduling conflicts and ensures the appropriate reagents are available and at the correct temperatures for immediate use (having samples sit while we bring reagents to temperature may negatively impact data). We will make effort to accommodate appointments made 24-48 hours before submission, but we cannot guarantee availability.
To schedule a submission appointment, please contact Evelyn Ng at microarrays@yale.edu.
Service Fees
| Type of Service | Yale Fee | Non-Yale* Fee |
|---|---|---|
| ParseBio scRNA Library Prep (starting from cDNA*, per 10k cells, without reagents) | $650 | $920 |
If the library prep starting from fixed cells, the first sublibrary prep cost would be $1050 for Yale users and $1426 for non-Yale users. The subsequent libraries would be $525 each for Yale and $713 each for non-Yale users.
Sequencing Request
To request sequencing at Yale Center for Genome Analysis with the prepared library, please use WiKiLIMS system.
WIKILIMS Login
Non-Yale Investigators
Please contact ycga@yale.edu with the details of your project (or call 203-737-3031) and you will be forwarded to the appropriate manager.
Once your workflow fully transitions to the wiki, you will be able to track sample progress in real time by either logging in or by automatic email updates.
The site works best using the LATEST version of Firefox or Chrome.
New Users
For new users, kindly request an account here and bookmark the page.
- Helpful resources for the WiKiLIMS system
- To give feedback, please email christopher.castaldi@yale.edu
Please submit service request through KMSR PPMS