Correlation between the amount of protein analyzed and the accuracy of MW determination
using DAWN® DSP and OPTILAB DSP detectors
Look at the actual data in the "micro analyses" graph
| Protein | Amount loaded [micrograms] | number of runs | Pred. MW [kDa] a | Average experimental MW[kDa] | Range of experimental accuracy[%]b | Standard deviation [kDa] |
|---|---|---|---|---|---|---|
| Ovalbumin | 150 | 4 | 42.8 | 42.4 | 0.2 to 1.6 | 0.3 |
| 100 | 7 | 42.8 | 42.3 | 0.2 to 2.4 | 0.8 | |
| 45-50 | 4 | 42.8 | 41.6 | 0.5 to 5.8 | 1 | |
| 6-10 | 5 | 42.8 | 42.9 | 1.4 to 4.5 | 1.5 | |
| Transferrin | 100 | 3 | 75.2 | 76.5 | 0.7 to 3.2 | 1 |
| 8 | 5 | 75.2 | 76.3 | 0.3 to 5.2 | 2 |
(column: TSK GEL G3000SWXL [TosoHaas], buffer: 20 mM phosphate, 150 mM NaCl, pH=7.5).
a predicated MW was calculated based on protein sequence as retrieved from the ExPASy molecular biology WWW server of the Swiss Institute of Bioinformatics (SIB). http://expasy.hcuge.ch/
b Experimental accuracy (%) is calculated as an absolute value of {100*[(Average Experimental MW-predicted MW)/predicted MW]}