Morphology Core Facility

The Morphology Core Facility provides instrumentation and technical expertise for the preparation, acquisition and analysis of images of cells and tissues at the light microscopic level. Given the cost of such instrumentation and the high level of technical expertise required to perform these investigational techniques, this Core was established to ensure the availability of these techniques for Center members. In recognition of the broad usefulness of this Core facility, the School of Medicine has partnered with the Liver Center by making ongoing, major investments to ensure that the facility remains state-of-the-art.

Specific Activities and Services Available

  • Confocal Microscopy
  • Two Photon Microscopy
  • Super-resolution Microscopy
  • Swept Field Microscope
  • Light Sheet Microscope
  • Electron Microscopy
  • Image Processing and Analysis
  • Other microscopy tools including: epifluorescence microscopy, cryosectioning, and brightfield microscopy

Equipment

Confocal, Super-resolution, and Multiphoton Imaging:
  • Leica SP8 Gated STED super-resolution microscope
  • Zeiss LSM 880 AiryScan Fast
  • Bruker PALM/STORM
  • Swept-field confocal microscope
  • Bruker Vutara 352 super-resolution microscope
  • Zeiss LSM 710 duo combined confocal and multiphoton microscope
  • Leica SP5 confocal microscope

In addition to the imaging acquisition equipment, the Morphology Core also houses 4 PC workstations dedicated to image analysis. Software from Zeiss (ZEN blue), Leica (LAS X), Bitplane (Imaris), SVI (Huygens Deconvolution) and Perkin Elmer (Volocity) are available to users for post-acquisition image analysis, which include modules for fluorescence deconvolution, 3D rendering, measurements of time lapse data and fluorescence co-localization. Technical expertise is available to aid in the use of the equipment and image analysis software.

Electron Microscopy:

  • Tecnai 12 Biotwin
  • FEI Tecnai TF20 FEG, 200kV TEM/STEM with EDAX attachment

Epifluorescence Microscopy, including quantitative and ratio imaging:

  • Zeiss Axio Observer epifluorescence microscope
  • Olympus BX51 multi-headed brightfield microscope
  • Zeiss Discovery 8 SteReo dissecting microscope
  • Leica CN-1860 cryostat

For training and access to this equipment, please contact Al Mennone.

<<Cellular and Molecular Physiology Core Facility Clinical Translation Facility>>

Morphology Core Director

Michael Harris Nathanson, MD, PhD

Gladys Phillips Crofoot Professor of Medicine (Digestive Diseases) and Professor of Cell Biology

Contact Info

michael.nathanson@yale.edu