2015
Establishment and Characterization of Rat Portal Myofibroblast Cell Lines
Fausther M, Goree JR, Lavoie ÉG, Graham AL, Sévigny J, Dranoff JA. Establishment and Characterization of Rat Portal Myofibroblast Cell Lines. PLOS ONE 2015, 10: e0121161. PMID: 25822334, PMCID: PMC4378927, DOI: 10.1371/journal.pone.0121161.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBromodeoxyuridineCell Culture TechniquesCell LineFluorescent Antibody TechniqueImmunoblottingLiverMyofibroblastsPortal SystemRatsReverse Transcriptase Polymerase Chain ReactionConceptsHepatic stellate cellsPortal fibroblastsMyofibroblast cell lineLiver fibrosisCell linesAlpha 1Alpha-smooth muscle actinMyofibroblast marker alpha-smooth muscle actinScar-forming myofibroblastsSmooth muscle actinMesenchymal cell markersNTPDase2/CD39L1Lecithin retinol acyltransferaseStellate cellsCollagen alpha 1Cholangiocyte proliferationMetalloproteinases-1Muscle actinTissue inhibitorAdult rat liverCell markersCholangiocarcinoma cellsCulture activationRetinol acyltransferaseFunctional studies
2008
IL-6 downregulates transcription of NTPDase2 via specific promoter elements
Yu J, Lavoie E, Sheung N, Tremblay JJ, Sévigny J, Dranoff JA. IL-6 downregulates transcription of NTPDase2 via specific promoter elements. AJP Gastrointestinal And Liver Physiology 2008, 294: g748-g756. PMID: 18202114, PMCID: PMC5239663, DOI: 10.1152/ajpgi.00208.2007.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBlotting, WesternCell DifferentiationCloning, MolecularCytokine Receptor gp130DNA, ComplementaryDown-RegulationElectrophoretic Mobility Shift AssayFibroblastsFluorescent Antibody TechniqueInterleukin-6LuciferasesMaleMicroscopy, ConfocalMutagenesis, Site-DirectedPromoter Regions, GeneticRatsRats, Sprague-DawleyResponse ElementsReverse Transcriptase Polymerase Chain ReactionConceptsBile ductular proliferationPortal fibroblastsIL-6Ductular proliferationBiliary cirrhosisIL-6 receptor gp80Alpha-smooth muscle actin expressionIL-6 responsePotential therapeutic approachMuscle actin expressionNTPDase2 expressionTime-dependent fashionBiliary fibrosisIL-6 receptor gp130Interleukin-6Therapeutic approachesResponse elementMyofibroblastic differentiationDiphosphohydrolase 2CirrhosisMRNA expressionActin expressionMinimal promoter constructProtein expressionIL-6 response element
2007
Succinate is a paracrine signal for liver damage
Correa PR, Kruglov EA, Thompson M, Leite MF, Dranoff JA, Nathanson MH. Succinate is a paracrine signal for liver damage. Journal Of Hepatology 2007, 47: 262-269. PMID: 17451837, PMCID: PMC1986575, DOI: 10.1016/j.jhep.2007.03.016.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFluorescent Antibody TechniqueIn Vitro TechniquesInfusions, IntravenousIschemiaLiverLiver DiseasesMaleParacrine CommunicationPerfusionPortal VeinPressureRatsRats, Sprague-DawleyReceptors, G-Protein-CoupledReverse Transcriptase Polymerase Chain ReactionSignal TransductionSuccinic AcidTissue DistributionConceptsHepatic stellate cellsSuccinate receptorParacrine signalsStellate cell activationStellate cellsCell expression systemTime-lapse imagingRelease of succinateCell activationCytosolic Ca2Effect of succinatePrimary hepatic stellate cellsHepatic cell typesExpression systemQuiescent hepatic stellate cellsConfocal immunofluorescencePhysiological roleIschemic hepatocytesCell typesBiochemical assaysSingle cellsLiver damageBACKGROUND/Western blotCAMP production