2017
Transforming Growth Factors α and β Are Essential for Modeling Cholangiocarcinoma Desmoplasia and Progression in a Three-Dimensional Organotypic Culture Model
Manzanares MÁ, Usui A, Campbell DJ, Dumur CI, Maldonado GT, Fausther M, Dranoff JA, Sirica AE. Transforming Growth Factors α and β Are Essential for Modeling Cholangiocarcinoma Desmoplasia and Progression in a Three-Dimensional Organotypic Culture Model. American Journal Of Pathology 2017, 187: 1068-1092. PMID: 28315313, PMCID: PMC5417049, DOI: 10.1016/j.ajpath.2017.01.013.Peer-Reviewed Original ResearchConceptsIntrahepatic cholangiocarcinomaOrganotypic culture modelDesmoplastic reactionThree-dimensional organotypic culture modelsCulture modelMesenchymal cell originCholangiocarcinoma cell growthCancer-associated myofibroblastsGrowth factor αAggressive malignancyDense fibrocollagenous stromaMalignant gradingCell anaplasiaSitu tumors
2002
The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver
Dranoff JA, Kruglov EA, Robson SC, Braun N, Zimmermann H, Sévigny J. The ecto‐nucleoside triphosphate diphosphohydrolase NTPDase2/CD39L1 is expressed in a novel functional compartment within the liver. Hepatology 2002, 36: 1135-1144. PMID: 12395323, DOI: 10.1053/jhep.2002.36823.Peer-Reviewed Original ResearchConceptsIntrahepatic bile ductsExtracellular nucleotidesBile ductDiverse biological functionsBlot analysisEcto-nucleoside triphosphate diphosphohydrolasesNTPDase2/CD39L1Portal fibroblastsNorthern blot analysisCellular compartmentsBiological functionsPotential regulatorConfocal immunofluorescenceWestern blot analysisHepatic blood flowBile duct epitheliumReverse transcription-polymerase chain reactionFunctional assaysTriphosphate diphosphohydrolasesImmunoelectron microscopyFunctional compartmentsHepatic central veinNucleotidesNTPDase1NTPDase2