2020
Purinergic Regulation of Bile Ductular Secretion
Dranoff J. Purinergic Regulation of Bile Ductular Secretion. 2020, 96-104. DOI: 10.1201/9780367813888-9.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsP2Y receptorsPurinergic receptorsIntestinal epitheliumExtracellular nucleotidesP2Y receptor subtypesP2 receptorsReceptor subtypesRespiratory epitheliumP1 receptorsMucin secretionNucleotide receptorsPurinergic agonistsPlatelet aggregationPurinergic regulationChloride secretionDistinct receptorsDuctular secretionNucleoside receptorsEpithelial secretionEpitheliumReceptorsSalivary glandsSecretionSecretory epitheliumRenal epithelium
2014
Expression of mediators of purinergic signaling in human liver cell lines
Goree JR, Lavoie EG, Fausther M, Dranoff JA. Expression of mediators of purinergic signaling in human liver cell lines. Purinergic Signalling 2014, 10: 631-638. PMID: 25194703, PMCID: PMC4272373, DOI: 10.1007/s11302-014-9425-4.Peer-Reviewed Original ResearchConceptsLiver cell subpopulationsPurinergic signalingPurinergic signalsCell subpopulationsCell linesExpression of mediatorsLiver disease pathogenesisHuman liver cell lineHuman cell line modelsCell line modelsLiver cell lineHepatic functionP2Y receptorsP2X receptorsLX-2Disease pathogenesisAdenosine receptorsLiver physiologyRT-PCRReceptorsHuman cell linesPurinergicLiverSubpopulationsSignaling
2012
Role of purinergic P2X receptors in the control of liver homeostasis
Fausther M, Gonzales E, Dranoff JA. Role of purinergic P2X receptors in the control of liver homeostasis. Wiley Interdisciplinary Reviews Membrane Transport And Signalling 2012, 1: 341-348. PMID: 22662313, PMCID: PMC3364537, DOI: 10.1002/wmts.32.Peer-Reviewed Original ResearchBasic cellular functionsIntracellular signal transduction pathwaysNumerous membrane transportersSignal transduction pathwaysLiver homeostasisPurinergic signalingCellular functionsP2Y receptorsTissue homeostasisTransduction pathwaysP2X receptorsHepatic cellsMembrane transportersMammalian speciesCell cycleSignaling pathwaysSalvage pathwayProtein synthesisCell typesATP moleculesFunctional relevanceExtracellular mediumMembrane levelGlucose/lipid metabolismDifferent liver cell types
2009
Transcriptional regulation of IL-6 in bile duct epithelia by extracellular ATP
Yu J, Sheung N, Soliman EM, Spirli C, Dranoff JA. Transcriptional regulation of IL-6 in bile duct epithelia by extracellular ATP. AJP Gastrointestinal And Liver Physiology 2009, 296: g563-g571. PMID: 19136380, PMCID: PMC2660176, DOI: 10.1152/ajpgi.90502.2008.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphateAnimalsAntibodiesBile DuctsCalciumCalcium SignalingCell Line, TransformedCell Line, TumorCyclic AMPEpithelial CellsExtracellular SpaceFibroblastsHumansImmunoblottingInterleukin-6MaleMutagenesis, Site-DirectedPromoter Regions, GeneticRatsRats, Sprague-DawleyReceptors, Purinergic P2Response ElementsRNA, MessengerSignal TransductionTranscriptional ActivationConceptsBile duct epitheliumIL-6IL-6 transcriptionDuct epitheliumLiver injuryCAMP response elementP2Y11 receptorInflammatory cytokines IL-6Extracellular ATPIL-6 upregulationUse of agonistsRat bile duct epitheliaCytokines IL-6IL-6 releaseIL-6 promoter activityIL-6 mRNAExtracellular ATP actsCalcium agonistP2Y receptorsPharmacological profileHepatic responseCalcium-dependent fashionExtracellular nucleotidesCytosolic calciumPurinergic signals
2007
Molecular basis for calcium signaling in hepatic stellate cells
Kruglov EA, Correa PR, Arora G, Yu J, Nathanson MH, Dranoff JA. Molecular basis for calcium signaling in hepatic stellate cells. AJP Gastrointestinal And Liver Physiology 2007, 292: g975-g982. PMID: 17204544, DOI: 10.1152/ajpgi.00401.2006.Peer-Reviewed Original ResearchMeSH KeywordsActive Transport, Cell NucleusAdenosine TriphosphateAnimalsCalcium SignalingCalreticulinCell NucleusCell ShapeCell Surface ExtensionsCells, CulturedEndoplasmic ReticulumInositol 1,4,5-Trisphosphate ReceptorsLiverLiver CirrhosisMaleMicroscopy, ConfocalMicroscopy, VideoRatsRats, Sprague-DawleyReceptors, Purinergic P2RNA, MessengerTime FactorsConceptsHepatic stellate cellsCell extensionsLipid-storing cellsSubcellular organizationLiver fibrosisMolecular basisStellate cellsSubcellular signalingTrisphosphate receptorChronic liver failureProgressive liver fibrosisSufficient machineryExtracellular ATPMyofibroblastic transdifferentiationOrgan fibrosisLiver failureP2Y receptorsHealthy liverATPLocal controlCellsCritical stepLocal applicationImportant mediatorFibrosis
2005
Portal Fibroblasts Regulate the Proliferation of Bile Duct Epithelia via Expression of NTPDase2*
Jhandier MN, Kruglov EA, Lavoie É, Sévigny J, Dranoff JA. Portal Fibroblasts Regulate the Proliferation of Bile Duct Epithelia via Expression of NTPDase2*. Journal Of Biological Chemistry 2005, 280: 22986-22992. PMID: 15799977, DOI: 10.1074/jbc.m412371200.Peer-Reviewed Original ResearchMeSH KeywordsAdenosine TriphosphatasesAnimalsBile DuctsBromodeoxyuridineCell ProliferationCholangiocarcinomaCholestasisCoculture TechniquesDNA, ComplementaryEpithelial CellsFibroblastsHumansLiverMaleMicroscopy, ConfocalMicroscopy, FluorescenceModels, BiologicalRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingSignal TransductionTransfectionConceptsBile ductular proliferationExpression of NTPDase2Portal fibroblastsDuctular proliferationBile duct epitheliumNTPDase2 expressionMz-ChA-1 cellsPortal myofibroblastsP2Y receptorsDuct epitheliumBile duct-ligated ratsCell proliferationDuct-ligated ratsReal-time reverse transcription PCRQuantitative real-time reverse transcription PCRHuman cholangiocarcinoma cellsNovel co-culture modelMz-ChA-1 human cholangiocarcinoma cellsNucleotidase apyraseP2Y activationCo-culture modelObstructive cholestasisReverse transcription-PCRPathologic alterationsEpithelial proliferation
2004
Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells
Dranoff JA, Ogawa M, Kruglov EA, Gaça M, Sévigny J, Robson SC, Wells RG. Expression of P2Y nucleotide receptors and ectonucleotidases in quiescent and activated rat hepatic stellate cells. AJP Gastrointestinal And Liver Physiology 2004, 287: g417-g424. PMID: 14764443, PMCID: PMC5241161, DOI: 10.1152/ajpgi.00294.2003.Peer-Reviewed Original ResearchConceptsHepatic stellate cellsActivated hepatic stellate cellsQuiescent hepatic stellate cellsP2Y receptorsExtracellular nucleotidesP2Y subtypesStellate cellsMRNA expressionRT-PCRInhibitor suraminP2Y receptor subtypesReceptor subtype expressionExpression of mediatorsExpression of receptorsFunctional P2Y receptorsRat hepatic stellate cellsReal-time RT-PCRP2Y nucleotide receptorsEctonucleotidase expressionLiver fibrosisReceptor subtypesSubtype expressionEctonucleotidase activityExtracellular UDPFibrogenic cells
2001
Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia
Dranoff J, Masyuk A, Kruglov E, LaRusso N, Nathanson M. Polarized expression and function of P2Y ATP receptors in rat bile duct epithelia. AJP Gastrointestinal And Liver Physiology 2001, 281: g1059-g1067. PMID: 11557527, DOI: 10.1152/ajpgi.2001.281.4.g1059.Peer-Reviewed Original ResearchConceptsP2Y receptor subtypesP2Y receptorsReceptor subtypesIntrahepatic bile duct unitsATP receptorsBile duct epitheliumMultiple P2Y receptor subtypesRat bile duct epitheliaBile duct unitsImportant regulatorLuminal ATPLuminal perfusionMolecular subtypesBile secretionDuct epitheliumBasolateral additionExtracellular nucleotidesDuctular secretionSubtypesCytosolic Ca2Duct unitsRT-PCRCholangiocytesReceptorsSecretion
2000
A Primitive ATP Receptor from the Little Skate Raja erinacea *
Dranoff J, O'Neill A, Franco A, Cai S, Connolly G, Ballatori N, Boyer J, Nathanson M. A Primitive ATP Receptor from the Little Skate Raja erinacea *. Journal Of Biological Chemistry 2000, 275: 30701-30706. PMID: 10900200, DOI: 10.1074/jbc.m003366200.Peer-Reviewed Original ResearchConceptsLittle skate Raja erinaceaSkate Raja erinaceaDegenerate oligonucleotide probeRaja erinaceaP2Y receptor pharmacologySingle polymerase chain reaction productXenopus oocyte expression systemLiver cDNA libraryATP receptorsHigher organismsEvolutionary forerunnerCommon ancestorOocyte expression systemPhylogenetic analysisP2Y receptorsCDNA clonesPrimitive organismsCDNA libraryGene productsSubstrate preferenceMolecular basisExpression systemNucleotide specificityMammalian tissuesRange of nucleotides