2014
Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation
Shibata S, Arroyo JP, Castañeda-Bueno M, Puthumana J, Zhang J, Uchida S, Stone KL, Lam TT, Lifton RP. Angiotensin II signaling via protein kinase C phosphorylates Kelch-like 3, preventing WNK4 degradation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2014, 111: 15556-15561. PMID: 25313067, PMCID: PMC4217463, DOI: 10.1073/pnas.1418342111.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAmino Acid SequenceAngiotensin IIAnimalsCarrier ProteinsCell LineHumansKidneyMice, Inbred C57BLMicrofilament ProteinsMolecular Sequence DataPhosphorylationPhosphoserineProtein BindingProtein Kinase CProtein Serine-Threonine KinasesProteolysisSignal TransductionConceptsRenal salt reabsorptionAngiotensin IIVolume depletionSalt reabsorptionNormal physiologic responseProtein kinase CAII administrationBlood pressureCardiovascular diseaseGlobal burdenPhysiologic responsesCullin 3Kinase CNaCl cotransporterReabsorptionHuman genetic studiesSecretionHypertensionNormal mechanismsWNK4 degradationMissense mutationsSerine 433WNK4Inverse relationshipCultured cells
2013
Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4
Shibata S, Zhang J, Puthumana J, Stone KL, Lifton RP. Kelch-like 3 and Cullin 3 regulate electrolyte homeostasis via ubiquitination and degradation of WNK4. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 7838-7843. PMID: 23576762, PMCID: PMC3651502, DOI: 10.1073/pnas.1304592110.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCarrier ProteinsChlorocebus aethiopsCOS CellsCullin ProteinsElectrolytesGene Expression RegulationHomeostasisHumansIntracellular Signaling Peptides and ProteinsMicrofilament ProteinsMinor Histocompatibility AntigensMutationPotassium Channels, Inwardly RectifyingProtein BindingProtein Serine-Threonine KinasesProteomicsPseudohypoaldosteronismUbiquitinUbiquitinationWNK Lysine-Deficient Protein Kinase 1ConceptsPseudohypoaldosteronism type IICullin 3Disease-causing mutationsWNK4 levelsE3 ubiquitin ligase complexUbiquitin ligase complexDominant disease-causing mutationsRare Mendelian syndromePHAII-causing mutationsLigase complexTarget ubiquitinationUbiquitinationRenal salt reabsorptionMolecular explanationMendelian syndromesSpecific substratesCell surfaceWNK4Renal outer medullaryMutationsProtein levelsKelchKLHL3Specific roleElectrolyte homeostasis