2012
Tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone initiates and enhances pancreatitis responses
Alexandre M, Uduman AK, Minervini S, Raoof A, Shugrue CA, Akinbiyi EO, Patel V, Shitia M, Kolodecik TR, Patton R, Gorelick FS, Thrower EC. Tobacco carcinogen 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone initiates and enhances pancreatitis responses. AJP Gastrointestinal And Liver Physiology 2012, 303: g696-g704. PMID: 22837343, PMCID: PMC3468532, DOI: 10.1152/ajpgi.00138.2012.Peer-Reviewed Original ResearchConceptsNicotinic acetylcholine receptorsAcetylcholine receptorsCigarette smoke toxinsParameters of pancreatitisPancreatitis responsesTobacco carcinogen 4Acinar cell responsesRat pancreatic aciniSmoke toxinsAcute pancreatitisCigarette smokingIntraperitoneal injectionAcinar cell preparationsClinical studiesLong-term effectsCarcinogen 4Pancreatitis modelAdrenergic receptorsReceptor typesCell responsesTobacco toxinsPyknotic nucleiNNKPancreatic aciniPancreatitis
2003
Effect of ligands that increase cAMP on caerulein-induced zymogen activation in pancreatic acini
Lu Z, Kolodecik TR, Karne S, Nyce M, Gorelick F. Effect of ligands that increase cAMP on caerulein-induced zymogen activation in pancreatic acini. AJP Gastrointestinal And Liver Physiology 2003, 285: g822-g828. PMID: 12881228, PMCID: PMC2830556, DOI: 10.1152/ajpgi.00213.2003.Peer-Reviewed Original ResearchConceptsCCK analogue caeruleinEffect of CCKAcinar cellsSupraphysiological concentrationsPancreatic aciniCell cAMP levelsRat pancreatic aciniRp-8-BrPancreatic acinar cellsPathological activationCell-permeable cAMP analogCAMP levelsPhysiological concentrationsPancreatitisCell cAMPCCKZymogen activationActivationCAMP analogPhysiological ligandsAciniCells
1998
Telenzepine-sensitive muscarinic receptors on rat pancreatic acinar cells
Schmid S, Modlin I, Tang L, Stoch A, Rhee S, Nathanson M, Scheele G, Gorelick F. Telenzepine-sensitive muscarinic receptors on rat pancreatic acinar cells. American Journal Of Physiology 1998, 274: g734-g741. PMID: 9575856, DOI: 10.1152/ajpgi.1998.274.4.g734.Peer-Reviewed Original ResearchConceptsPancreatic acinar cellsAmylase secretionRat pancreatic acinar cellsMuscarinic receptorsAcinar cellsDifferent muscarinic receptor antagonistsM2 antagonist methoctramineMaximal amylase secretionMuscarinic receptor antagonistIsolated rat pancreatic aciniRat pancreatic aciniAntagonist methoctramineM3 subtypeM1 antagonistMuscarinic subtypesReceptor antagonistZymogen processingAntagonist sensitivityPancreatic aciniSecretionAntagonistSubtypesPotent inhibitorReceptorsDistinct patterns
1997
Distribution of a Synthetic Protease Inhibitor in Rat Pancreatic Acini After Supramaximal Secretagogue Stimulation
Otani T, Atomi Y, Kuroda A, Muto T, Tamura M, Fukuda S, Akao S, Gorelick F. Distribution of a Synthetic Protease Inhibitor in Rat Pancreatic Acini After Supramaximal Secretagogue Stimulation. Pancreas 1997, 14: 142-149. PMID: 9057186, DOI: 10.1097/00006676-199703000-00006.Peer-Reviewed Original ResearchConceptsAcinar cellsPancreatic aciniProtease inhibitorsLow molecular weight protease inhibitorSynthetic protease inhibitorLarge cytoplasmic vacuolesIsolated pancreatic aciniRat pancreatic aciniAcute pancreatitisWeight protease inhibitorsZymogen granulesCerulein treatmentE3123Supramaximal secretagogue stimulationVivo modelElectron microscopic autoradiographyBeneficial effectsMin of incubationUnstimulated controlsMicroscopic autoradiographyInhibition of proteasesCytoplasmic vacuolesAciniPancreatitisFormation of vacuoles
1993
Characterization of cAMP‐dependent protein kinase activation by CCK in rat pancreas
Marino C, Leach S, Schaefer J, Miller L, Gorelick F. Characterization of cAMP‐dependent protein kinase activation by CCK in rat pancreas. FEBS Letters 1993, 316: 48-52. PMID: 7678554, PMCID: PMC2830555, DOI: 10.1016/0014-5793(93)81734-h.Peer-Reviewed Original ResearchConceptsCAMP-dependent protein kinase activityProtein kinase activityKinase activityCAMP second messenger cascadeCAMP-dependent protein kinase activationProtein kinase activationSecond messenger cascadesCellular cAMP levelsKinase activationCAMP cascadeTreatment of aciniMessenger cascadesCellular cAMPCAMP levelsEnzyme secretionCascadeNew sensitive assayRat pancreatic aciniSensitive assayPancreatic aciniActivityHigh concentrationsAciniCellsActivation
1991
Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin.
Leach SD, Modlin IM, Scheele GA, Gorelick FS. Intracellular activation of digestive zymogens in rat pancreatic acini. Stimulation by high doses of cholecystokinin. Journal Of Clinical Investigation 1991, 87: 362-366. PMID: 1985109, PMCID: PMC295064, DOI: 10.1172/jci114995.Peer-Reviewed Original ResearchConceptsPancreatic aciniIntracellular activationCysteine protease inhibitor E64Digestive zymogensDose of cholecystokininRat pancreatic aciniProtease inhibitor E64Acute pancreatitisCholecystokininHigh dosesSerine protease activityAcidic subcellular compartmentsWeak base chloroquineImmunologic techniquesInhibitor E64Protonophore monensinPancreatitisIncubation mediumAciniBase chloroquineActivationIntracellular sitesProtease inhibitor benzamidineCell pelletsElectrophoretic mobility