2019
Recent Insights Into the Pathogenic Mechanism of Pancreatitis: Role of Acinar Cell Organelle Disorders.
Gukovskaya AS, Gorelick FS, Groblewski GE, Mareninova OA, Lugea A, Antonucci L, Waldron RT, Habtezion A, Karin M, Pandol SJ, Gukovsky I. Recent Insights Into the Pathogenic Mechanism of Pancreatitis: Role of Acinar Cell Organelle Disorders. Pancreas 2019, 48: 459-470. PMID: 30973461, PMCID: PMC6461375, DOI: 10.1097/mpa.0000000000001298.Peer-Reviewed Original ResearchConceptsOrganelle dysfunctionCell death responseSecretion of proteinsAcinar cell homeostasisOrganelle disordersNascent proteinsDysfunctional organellesDeath responseAccessory proteinsVesicular compartmentsEndosomal pathwayCell homeostasisAcute pancreatitisEndoplasmic reticulumProtein synthesisCells triggersPancreatic acinar cellsLethal inflammatory diseaseDigestive enzymesCell constituentsRecent insightsDistinct mechanismsProteinOrganellesAcinar cell injury
2018
Chapter 39 Structure-Function Relationships in the Pancreatic Acinar Cell
Gorelick F, Pandol S, Jamieson J. Chapter 39 Structure-Function Relationships in the Pancreatic Acinar Cell. 2018, 869-894. DOI: 10.1016/b978-0-12-809954-4.00039-6.Peer-Reviewed Original ResearchProtein synthesisEnzyme precursorsRodent acinar cellsAcinar cellsZymogen granulesCritical physiologic functionsSecretion of enzymesStructure-function relationshipsMajor cell typesCellular functionsCell biologistsVesicular transportCell signalingGolgi complexHuman acinar cellsPhysiologic functionEndoplasmic reticulumDuct cellsCell organellesPancreatic acinar cellsCell typesEnzyme proteinDigestive enzymesEnzymeMajor physiologic function
2017
Human Pancreatic Acinar Cells Proteomic Characterization, Physiologic Responses, and Organellar Disorders in ex Vivo Pancreatitis
Lugea A, Waldron RT, Mareninova OA, Shalbueva N, Deng N, Su HY, Thomas DD, Jones EK, Messenger SW, Yang J, Hu C, Gukovsky I, Liu Z, Groblewski GE, Gukovskaya AS, Gorelick FS, Pandol SJ. Human Pancreatic Acinar Cells Proteomic Characterization, Physiologic Responses, and Organellar Disorders in ex Vivo Pancreatitis. American Journal Of Pathology 2017, 187: 2726-2743. PMID: 28935577, PMCID: PMC5718097, DOI: 10.1016/j.ajpath.2017.08.017.Peer-Reviewed Original ResearchConceptsOrganellar morphologyEndoplasmic reticulum stressProteomic characterizationEndolysosomal functionProteomic analysisMolecular mechanismsMitochondrial depolarizationTaurolithocholic acidPhysiological functionsMuscarinic acetylcholine receptor M3Acute pancreatitis patientsBile acid taurolithocholic acidMacrophage inhibitory factorReticulum stressDigestive enzymesMuscarinic agonist carbacholTumor necrosis factorPhysiological responsesSimilar pathological responsesAcinar preparationsAcinar cell responsesCell viabilityInflammatory mediatorsSimilar mechanismPancreatitis patients
2012
Chapter 49 Structure–function Relationships in the Pancreatic Acinar Cell
Gorelick F, Jamieson J. Chapter 49 Structure–function Relationships in the Pancreatic Acinar Cell. 2012, 1341-1360. DOI: 10.1016/b978-0-12-382026-6.00049-x.Peer-Reviewed Original ResearchProtein synthesisZymogen granulesAcinar cellsSecretion of enzymesStructure-function relationshipsNascent proteinsVesicular transportCell signalingEnzyme precursorsGolgi complexEndoplasmic reticulumPancreatic acinar cellsDigestive enzymesEnzymeModel systemExport protein synthesisVectorial mannerApical regionProteinCellsHormonal routesGranulesDigestionDietary proteinExocytosis
2011
Environmental and Genetic Stressors and the Unfolded Protein Response in Exocrine Pancreatic Function – A Hypothesis
Pandol SJ, Gorelick FS, Lugea A. Environmental and Genetic Stressors and the Unfolded Protein Response in Exocrine Pancreatic Function – A Hypothesis. Frontiers In Physiology 2011, 2: 8. PMID: 21483727, PMCID: PMC3070477, DOI: 10.3389/fphys.2011.00008.Peer-Reviewed Original ResearchProtein responseER stressProtein synthetic machineryUnfolded protein responseDigestive enzymesProtein synthetic capacityRecent findingsAdaptive UPREndoplasmic reticulum stressGenetic stressorsSynthetic machineryMammalian organsEnvironmental stressorsProtein synthesisKey pathwaysUPRReticulum stressExocrine cellsExocrine pancreasSynthetic capacityEnzymePathwayPathologic pathwaysStressorsMachinery
2009
Genetic and pharmacologic manipulation of vacuolar ATPase: Effects on zymogen activation in pancreatic acini
Kolodecik T, Gorelick F, Thrower E. Genetic and pharmacologic manipulation of vacuolar ATPase: Effects on zymogen activation in pancreatic acini. Open Access Animal Physiology 2009, Volume 1: 1-11. PMID: 21572923, PMCID: PMC3092382, DOI: 10.2147/oaap.s7252.Peer-Reviewed Original ResearchZymogen activationVacuolar ATPaseATP-dependent proton pumpTreatment of cellsGenetic approachesE subunitAcinar cellsProton pumpPancreatic acinar cellsPancreatitis responsesDigestive enzymesAcute pancreatitisPremature activationVATPaseSiRNAATPaseActivationSalicylihalamideCellsRecent studiesOrthologuesBaseline levelsHigh dosesPharmacologic manipulationAmylase secretion
2002
Neutrophils and NADPH oxidase mediate intrapancreatic trypsin activation in murine experimental acute pancreatitis
Gukovskaya AS, Vaquero E, Zaninovic V, Gorelick FS, Lusis AJ, Brennan M, Holland S, Pandol SJ. Neutrophils and NADPH oxidase mediate intrapancreatic trypsin activation in murine experimental acute pancreatitis. Gastroenterology 2002, 122: 974-984. PMID: 11910350, DOI: 10.1053/gast.2002.32409.Peer-Reviewed Original ResearchConceptsIntrapancreatic trypsin activationAcute pancreatitisNADPH oxidaseTrypsin activationAdenine Dinucleotide Phosphate OxidaseParenchymal cell injuryExperimental acute pancreatitisNADPH oxidase activityPancreatic acinar tissueDigestive enzymesWestern blot analysisAntineutrophil serumPathologic activationCell injuryPancreatitisExperimental pancreatitisHigh dosesMarked attenuationNeutrophilsAbstractTextAcinar tissuePancreatic homogenatesReactive oxygen speciesMyeloperoxidaseMice