2018
Subretinal Transplantation of Human Amniotic Epithelial Cells in the Treatment of Autoimmune Uveitis in Rats
Li J, Qiu C, Zhang Z, Yuan W, Ge Z, Tan B, Yang P, Liu J, Zhu X, Qiu C, Lai D, Guo L, Yu L. Subretinal Transplantation of Human Amniotic Epithelial Cells in the Treatment of Autoimmune Uveitis in Rats. Cell Transplantation 2018, 27: 1504-1514. PMID: 30168350, PMCID: PMC6180726, DOI: 10.1177/0963689718796196.Peer-Reviewed Original ResearchMeSH KeywordsAmnionAnimalsAutoimmune DiseasesCells, CulturedEpithelial CellsFemaleHumansMaleRats, Inbred LewRetinaUveitisConceptsExperimental autoimmune uveitisHuman amniotic epithelial cellsT cell subsetsAutoimmune uveitisOcular inflammatory diseaseEAU ratsEnzyme-linked immunosorbent assayAmniotic epithelial cellsSubretinal transplantationInflammatory diseasesSubretinal injectionTherapeutic strategiesDay 0Current immunosuppressive regimensIL-10 levelsEpithelial cellsT regulatory (Treg) cellsImmune cell infiltrationSlit-lamp microscopyMonocyte chemoattractant proteinSerious side effectsInterphotoreceptor retinoid-binding proteinLocal cytokine environmentNovel therapeutic strategiesAlternative therapeutic strategies
2017
Nucleoside/nucleotide reverse transcriptase inhibitors attenuate angiogenesis and lymphangiogenesis by impairing receptor tyrosine kinases signalling in endothelial cells
Song L, Ding S, Ge Z, Zhu X, Qiu C, Wang Y, Lai E, Yang W, Sun Y, Chow S, Yu L. Nucleoside/nucleotide reverse transcriptase inhibitors attenuate angiogenesis and lymphangiogenesis by impairing receptor tyrosine kinases signalling in endothelial cells. British Journal Of Pharmacology 2017, 175: 1241-1259. PMID: 28910489, PMCID: PMC5866989, DOI: 10.1111/bph.14036.Peer-Reviewed Original ResearchConceptsReceptor tyrosine kinase internalizationExcessive ROS levelsConfocal immunofluorescence microscopyEndothelial cellsAntiretroviral therapyReceptor tyrosine kinasesMitochondrial DNA copy numberLymphatic endothelial cellsQuantitative real-time PCRMigration of ECsMitochondrial oxidative stressRTK endocytosisDNA copy numberProtein maturationRTK signalsEarly endosomesTube formation assaysTyrosine kinaseCardiovascular diseaseNucleoside/Blood vessel growthReal-time PCRFGFR1 pathwayCopy numberImmunofluorescence microscopySUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling
Zhu X, Ding S, Qiu C, Shi Y, Song L, Wang Y, Wang Y, Li J, Wang Y, Sun Y, Qin L, Chen J, Simons M, Min W, Yu L. SUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling. Circulation Research 2017, 121: 636-649. PMID: 28760777, PMCID: PMC5581236, DOI: 10.1161/circresaha.117.310696.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBinding SitesCalcium-Binding ProteinsCells, CulturedCysteine EndopeptidasesEndopeptidasesHuman Umbilical Vein Endothelial CellsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceNeovascularization, PhysiologicProtein BindingReceptors, NotchReceptors, Vascular Endothelial Growth FactorSignal TransductionSumoylationConceptsEndothelial NotchPost-translational SUMO modificationCell-cell interaction mechanismRole of SUMOylationNotch signal activationDual-luciferase assayCotranscriptional factorsRegulation of angiogenesisTissue patterningCell fateSUMO conjugationSUMO modificationSignal transductionVEGF receptor signalingSUMOylationImmunoprecipitation analysisRegulatory mechanismsPathway functionDLL4 stimulationBiological eventsReceptor signalingSignal activationCultured endothelial cellsAngiogenic signalingSENP1