2023
Acetate controls endothelial-to-mesenchymal transition
Zhu X, Wang Y, Soaita I, Lee H, Bae H, Boutagy N, Bostwick A, Zhang R, Bowman C, Xu Y, Trefely S, Chen Y, Qin L, Sessa W, Tellides G, Jang C, Snyder N, Yu L, Arany Z, Simons M. Acetate controls endothelial-to-mesenchymal transition. Cell Metabolism 2023, 35: 1163-1178.e10. PMID: 37327791, PMCID: PMC10529701, DOI: 10.1016/j.cmet.2023.05.010.Peer-Reviewed Original ResearchMeSH KeywordsEndothelial CellsEndotheliumHumansSignal TransductionTransforming Growth Factor betaVascular DiseasesConceptsTGF-β signalingChronic vascular diseaseTGF-β receptor ALK5Mesenchymal transitionInduction of EndMTVascular diseaseMolecular basisPositive feedback loopReceptor ALK5Cellular levelSMADs 2Novel targetEndMT inductionMetabolic modulationMetabolic basisFibrotic stateSignalingPotential treatmentEndMTTGFDiseaseActivationInductionACSS2PDK4
2022
FGFR1 SUMOylation coordinates endothelial angiogenic signaling in angiogenesis
Zhu X, Qiu C, Wang Y, Jiang Y, Chen Y, Fan L, Ren R, Wang Y, Chen Y, Feng Y, Zhou X, Zhu Y, Ge Z, Lai D, Qin L, Simons M, Yu L. FGFR1 SUMOylation coordinates endothelial angiogenic signaling in angiogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2022, 119: e2202631119. PMID: 35733256, PMCID: PMC9245619, DOI: 10.1073/pnas.2202631119.Peer-Reviewed Original ResearchConceptsFGF receptor 1VEGFA/VEGFR2 signalingSmall ubiquitin-like modifier (SUMO) modificationSUMOylation-defective mutantReceptor complex formationTyrosine kinase activationVEGFA/VEGFR2Endothelial cellsFGF/FGFRRegulatory featuresEmbryonic developmentTissue homeostasisSUMOylationReversible SUMOylationKinase activationFibroblast growth factorBasic fibroblast growth factorFRS2αVascular developmentVEGFR2 signalingContradictory phenotypesEndothelial sproutingAngiogenic signalingFunctional significanceProangiogenic stimuli
2017
SUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling
Zhu X, Ding S, Qiu C, Shi Y, Song L, Wang Y, Wang Y, Li J, Wang Y, Sun Y, Qin L, Chen J, Simons M, Min W, Yu L. SUMOylation Negatively Regulates Angiogenesis by Targeting Endothelial NOTCH Signaling. Circulation Research 2017, 121: 636-649. PMID: 28760777, PMCID: PMC5581236, DOI: 10.1161/circresaha.117.310696.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsBinding SitesCalcium-Binding ProteinsCells, CulturedCysteine EndopeptidasesEndopeptidasesHuman Umbilical Vein Endothelial CellsHumansIntracellular Signaling Peptides and ProteinsMembrane ProteinsMiceNeovascularization, PhysiologicProtein BindingReceptors, NotchReceptors, Vascular Endothelial Growth FactorSignal TransductionSumoylationConceptsEndothelial NotchPost-translational SUMO modificationCell-cell interaction mechanismRole of SUMOylationNotch signal activationDual-luciferase assayCotranscriptional factorsRegulation of angiogenesisTissue patterningCell fateSUMO conjugationSUMO modificationSignal transductionVEGF receptor signalingSUMOylationImmunoprecipitation analysisRegulatory mechanismsPathway functionDLL4 stimulationBiological eventsReceptor signalingSignal activationCultured endothelial cellsAngiogenic signalingSENP1