Electroporative transfection with KGF-1 DNA improves wound healing in a diabetic mouse model
Marti G, Ferguson M, Wang J, Byrnes C, Dieb R, Qaiser R, Bonde P, Duncan M, Harmon J. Electroporative transfection with KGF-1 DNA improves wound healing in a diabetic mouse model. Gene Therapy 2004, 11: 1780-1785. PMID: 15470477, DOI: 10.1038/sj.gt.3302383.Peer-Reviewed Original ResearchConceptsKeratinocyte growth factorDiabetic mouse modelMouse modelWound healing modelExcisional woundsWound closureEpithelial cell proliferationRT-PCR assaysDiabetic miceDiabetic individualsUnresolved inflammationDNA injectionMouse wound-healing modelUntreated groupSingle injectionDay 12KGF mRNAELISA assaysKGF proteinHistological analysisGrowth factorSubsequent electroporationNaked DNA injectionCell proliferationWoundsKGF-1 plasmid delivered with electroporation accelerates wound closure in diabetic mice
Marti G, Lin M, Qaiser R, Dieb R, Wang J, Parth S, Bonde P, Duncan M, Harmon J. KGF-1 plasmid delivered with electroporation accelerates wound closure in diabetic mice. Journal Of The American College Of Surgeons 2004, 199: 59. DOI: 10.1016/j.jamcollsurg.2004.05.121.Peer-Reviewed Original ResearchDiabetic miceGrowth factorKGF-1Diabetic mouse modelWound healing deficitsUnmet medical challengeKeratinocyte growth factorFull-thickness woundsImportant growth factorDiabetic individualsHistological assessmentHealing deficitsMouse modelEP groupClosure of woundsGrading scaleMedical challengeDNA expression vectorsThickness woundsWound areaDiminished levelsWoundsUntreated woundsMiceCMV promoter