2021
Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering
Ellis MW, Riaz M, Huang Y, Anderson CW, Luo J, Park J, Lopez CA, Batty LD, Gibson KH, Qyang Y. Epigallocatechin gallate facilitates extracellular elastin fiber formation in induced pluripotent stem cell derived vascular smooth muscle cells for tissue engineering. Journal Of Molecular And Cellular Cardiology 2021, 163: 167-174. PMID: 34979103, PMCID: PMC8920537, DOI: 10.1016/j.yjmcc.2021.12.014.Peer-Reviewed Original ResearchMeSH KeywordsCatechinElastinHumansInduced Pluripotent Stem CellsMuscle, Smooth, VascularMyocytes, Smooth MuscleReproducibility of ResultsTissue EngineeringConceptsPluripotent stem cellsTissue engineeringStem cell derivativesPluripotent stem cell derivativesInduced pluripotent stem cellsStem cellsGraft productionMechanical strengthExtracellular formationExpression systemCell derivativesVascular smooth muscle cellsElastin fiber formationEngineered graftSmooth muscle cellsFiber formationNotable obstacleLack of elastinMuscle cellsEngineeringClinical applicationVascular graftsCell proliferative capacityElastin productionProliferative capacityMethods for Differentiating hiPSCs into Vascular Smooth Muscle Cells
Li ML, Luo J, Ellis MW, Riaz M, Ajaj Y, Qyang Y. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells. Methods In Molecular Biology 2021, 2375: 21-34. PMID: 34591296, DOI: 10.1007/978-1-0716-1708-3_3.Peer-Reviewed Original ResearchMeSH KeywordsCell DifferentiationHumansInduced Pluripotent Stem CellsMuscle, Smooth, VascularMyocytes, Smooth MuscleTissue EngineeringConceptsHuman induced pluripotent stem cellsVascular smooth muscle cellsPluripotent stem cellsLateral plate mesodermEarly embryonic developmentStem cellsSmooth muscle cellsHuman pluripotent stem cellsInduced pluripotent stem cellsExtracellular matrix proteinsMuscle cellsMesoderm lineagePlate mesodermEmbryonic developmentVascular cell sourceEmbryoid bodiesEB formationMatrix proteinsCellular interactionsDisease modelingPhysiological characteristicsVascular tissueTissue-engineered vascular graftsCell-based therapiesCell replacement
2020
Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering
Luo J, Lin Y, Shi X, Li G, Kural MH, Anderson CW, Ellis MW, Riaz M, Tellides G, Niklason LE, Qyang Y. Xenogeneic-free generation of vascular smooth muscle cells from human induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2020, 119: 155-168. PMID: 33130306, PMCID: PMC8168373, DOI: 10.1016/j.actbio.2020.10.042.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationHumansInduced Pluripotent Stem CellsMiceMuscle, Smooth, VascularMyocytes, Smooth MuscleTissue EngineeringConceptsVascular tissue engineeringTissue-engineered vascular graftsTissue engineeringComparable mechanical strengthVascular smooth muscle cellsMechanical strengthSmooth muscle cellsPolyglycolic acid scaffoldsTechnology one stepBiodegradable polyglycolic acid (PGA) scaffoldsXenogeneic-free conditionsAnimal-derived reagentsMuscle cellsVSMC differentiationImmunodeficient mouse modelEngineeringVascular graftsOne-stepStem cellsPluripotent stem cellsMouse modelCollagen depositionComparable capacityBlood vesselsAcid scaffoldsTissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs
Luo J, Qin L, Zhao L, Gui L, Ellis MW, Huang Y, Kural MH, Clark JA, Ono S, Wang J, Yuan Y, Zhang SM, Cong X, Li G, Riaz M, Lopez C, Hotta A, Campbell S, Tellides G, Dardik A, Niklason LE, Qyang Y. Tissue-Engineered Vascular Grafts with Advanced Mechanical Strength from Human iPSCs. Cell Stem Cell 2020, 26: 251-261.e8. PMID: 31956039, PMCID: PMC7021512, DOI: 10.1016/j.stem.2019.12.012.Peer-Reviewed Original ResearchMeSH KeywordsBlood Vessel ProsthesisHumansInduced Pluripotent Stem CellsMyocytes, Smooth MuscleTissue Engineering