Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population
Thompson R, Boraas L, Sowder M, Bechtel M, Orwin E. Three-Dimensional Cell Culture Environment Promotes Partial Recovery of the Native Corneal Keratocyte Phenotype from a Subcultured Population. Tissue Engineering Part A 2013, 19: 1564-1572. PMID: 23410050, PMCID: PMC3665321, DOI: 10.1089/ten.tea.2012.0084.Peer-Reviewed Original ResearchConceptsAlpha-smooth muscle actinOnly current treatmentFourth leading causePartial recoveryAldehyde dehydrogenase 1A1World Health OrganizationThree-dimensional (3D) cell culture environmentsProinflammatory cytokinesCorneal markersCorneal opacityQuantitative real-time PCRLeading causeCurrent treatmentReal-time PCRCorneal diseaseALDH1A1 expressionRabbit corneal keratocytesTissue transplantsCorneal phenotypeMuscle actinWestern blotPresence of serumActin expressionHealth OrganizationCulture conditions