1997
Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites
Sumpio B, Chang R, Xu W, Wang X, Du W. Regulation of tPA in endothelial cells exposed to cyclic strain: role of CRE, AP-2, and SSRE binding sites. American Journal Of Physiology 1997, 273: c1441-c1448. PMID: 9374627, DOI: 10.1152/ajpcell.1997.273.5.c1441.Peer-Reviewed Original ResearchAnimalsAorta, ThoracicBase SequenceBinding SitesCattleCell CycleCell NucleusCells, CulturedChloramphenicol O-AcetyltransferaseCyclic AMP Response Element-Binding ProteinDNA-Binding ProteinsEndothelium, VascularGene Expression Regulation, EnzymologicHumansKineticsMutagenesis, Site-DirectedOligodeoxyribonucleotidesPromoter Regions, GeneticRecombinant Fusion ProteinsStress, MechanicalTime FactorsTissue Plasminogen ActivatorTranscription Factor AP-2Transcription FactorsTransfection
1995
Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells.
Awolesi MA, Sessa WC, Sumpio BE. Cyclic strain upregulates nitric oxide synthase in cultured bovine aortic endothelial cells. Journal Of Clinical Investigation 1995, 96: 1449-1454. PMID: 7544806, PMCID: PMC185768, DOI: 10.1172/jci118181.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAortic endothelial cellsNitric oxide synthaseBovine aortic endothelial cellsCultured bovine aortic endothelial cellsEndothelial cellsOxide synthaseENOS transcriptsRegulation of eNOSENOS gene expressionBlot analysisHemodynamic forcesWestern blot analysisENOS levelsCycles/minImmunohistochemical stainingNitric oxideProtein levelsNorthern blot analysisNuclear runoff transcription assaysVivoCellsRunoff transcription assaysCytosolic RNAExpression