2008
Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells
Kim JI, Cordova AC, Hirayama Y, Madri JA, Sumpio BE. Differential Effects of Shear Stress and Cyclic Strain on Sp1 Phosphorylation by Protein Kinase Cζ Modulates Membrane Type 1–Matrix Metalloproteinase in Endothelial Cells. Endothelium 2008, 15: 33-42. PMID: 18568943, PMCID: PMC2644408, DOI: 10.1080/10623320802092260.Peer-Reviewed Original ResearchConceptsSp1 phosphorylationMT1-MMP expressionPromoter sitesPKCzeta inhibitorProtein kinase CzetaAffinity of Sp1Egr-1 bindingProtein kinase CζExtracellular matrix remodelingEndothelial cell migrationSp1Cell migrationPhosphorylationMatrix remodelingProtein expressionCyclic strainExpressionMembrane typeEndothelial cellsKey roleCzetaInhibitorsCζMetalloproteinaseAffinity
2005
Negative Regulation of Monocyte Adhesion to Arterial Elastic Laminae by Signal Regulatory Protein α and Src Homology 2 Domain-containing Protein-Tyrosine Phosphatase-1*
Liu SQ, Alkema PK, Tieché C, Tefft BJ, Liu DZ, Li YC, Sumpio BE, Caprini JA, Paniagua M. Negative Regulation of Monocyte Adhesion to Arterial Elastic Laminae by Signal Regulatory Protein α and Src Homology 2 Domain-containing Protein-Tyrosine Phosphatase-1*. Journal Of Biological Chemistry 2005, 280: 39294-39301. PMID: 16159885, DOI: 10.1074/jbc.m503866200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArteriesCell AdhesionElastic TissueIn Vitro TechniquesIntracellular Signaling Peptides and ProteinsLeukocytesMonocytesPhosphorylationProtein Phosphatase 1Protein Tyrosine Phosphatase, Non-Receptor Type 6Protein Tyrosine PhosphatasesRatsRats, Sprague-DawleyReceptors, Cell SurfaceRNA, Small InterferingConceptsSHP-1Arterial elastic laminaeSrc homology 2 domain-containing protein tyrosine phosphataseSIRP-alphaSrc homology 2 domain-containing protein tyrosine phosphatase 1Protein tyrosine phosphatase 1Protein tyrosine phosphataseSignal regulatory protein alphaSignal regulatory protein αRegulatory protein alphaExtracellular matrix constituentsMonocyte adhesionArterial morphogenesisPhosphatase 1Regulatory protein αNegative regulationProtein alphaProtein αImportant functionsInduced activationDegradation peptidesInhibitory effectElasticity of arteriesAdhesionMatrix constituents
2002
Cyclic Strain Stimulates Early Growth Response Gene Product 1–Mediated Expression of Membrane Type 1 Matrix Metalloproteinase in Endothelium
Yamaguchi S, Yamaguchi M, Yatsuyanagi E, Yun SS, Nakajima N, Madri JA, Sumpio BE. Cyclic Strain Stimulates Early Growth Response Gene Product 1–Mediated Expression of Membrane Type 1 Matrix Metalloproteinase in Endothelium. Laboratory Investigation 2002, 82: 949-956. PMID: 12118097, DOI: 10.1097/01.lab.0000020408.77307.e9.Peer-Reviewed Original ResearchTranscription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*
Yun S, Dardik A, Haga M, Yamashita A, Yamaguchi S, Koh Y, Madri JA, Sumpio BE. Transcription Factor Sp1 Phosphorylation Induced by Shear Stress Inhibits Membrane Type 1-Matrix Metalloproteinase Expression in Endothelium*. Journal Of Biological Chemistry 2002, 277: 34808-34814. PMID: 12093818, DOI: 10.1074/jbc.m205417200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceCells, CulturedDNADNA-Binding ProteinsEarly Growth Response Protein 1Electrophoretic Mobility Shift AssayEndothelium, VascularImmediate-Early ProteinsMatrix Metalloproteinases, Membrane-AssociatedMetalloendopeptidasesNogalamycinPhosphorylationPromoter Regions, GeneticRatsRats, Sprague-DawleyRNA, MessengerSp1 Transcription FactorStress, PhysiologicalTranscription FactorsConceptsMT1-MMP expressionEgr-1MRNA transcriptionMT1-MMP promoterPost-translational modificationsCalf intestinal phosphataseDistinct environmental stimuliTranscription factor expressionSp1 phosphorylationEgr-1 expressionSp1 DNAEndothelial cell migrationSerine phosphorylationPromoter sitesSp1Cell migrationEnvironmental stimuliMatrix remodelingIntestinal phosphataseProtein levelsTranscriptionTime-dependent fashionPhosphorylationMechanical forcesExpression